{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T16:54:41Z","timestamp":1780419281866,"version":"3.54.1"},"reference-count":75,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Two catalytic isoforms of the Na,K-ATPase, \u03b11 and \u03b14, are present in testis. While \u03b11 is ubiquitously expressed in tissues, \u03b14 predominates in male germ cells. Each isoform has distinct enzymatic properties and appears to play specific roles. To gain insight into the relevance of the Na,K-ATPase \u03b1 isoforms in male germ cell biology, we have studied the expression and activity of \u03b11 and \u03b14 during spermatogenesis and epididymal maturation. This was explored in rat testes at different ages, in isolated spermatogenic cells and in spermatozoa from the caput and caudal regions of the epididymis. Our results show that \u03b11 and \u03b14 undergo differential regulation during development. Whereas \u03b11 exhibits only modest changes, \u03b14 increases with gamete differentiation. The most drastic changes for \u03b14 take place in spermatocytes at the mRNA level, and with the transition of round spermatids into spermatozoa for expression and activity of the protein. No further changes are detected during transit of spermatozoa through the epididymis. In addition, the cellular distribution of \u03b14 is modified with development, being diffusely expressed at the plasma membrane and intracellular compartments of immature cells, finally to localize to the midregion of the spermatozoon flagellum. In contrast, the \u03b11 isoform is evenly present along the plasma membrane of the developing and mature gametes. In conclusion, the Na,K-ATPase \u03b11 and \u03b14 isoforms are functional in diploid, meiotic and haploid male germ cells, \u03b14 being significantly upregulated during spermatogenesis. These results support the importance of \u03b14 in male gamete differentiation and function.<\/jats:p>","DOI":"10.1530\/rep.1.00806","type":"journal-article","created":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T15:37:48Z","timestamp":1130859468000},"page":"627-641","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":63,"title":["Different expression and activity of the \u03b11 and \u03b14 isoforms of the Na,K-ATPase during rat male germ cell ontogeny"],"prefix":"10.1093","volume":"130","author":[{"given":"K","family":"Wagoner","sequence":"first","affiliation":[{"name":"Department of Molecular and Integrative Physiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative 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