{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:15:16Z","timestamp":1789380916522,"version":"build-2803163510"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Trans-placental transport of amino acids is vital for the developing fetus. Using the BeWo cell line as a placental model, we investigated the effect of restricting amino acid availability on amino acid transport system type A. BeWo cells were cultured either in amino acid-depleted (without non-essential amino acids) or control media for 1, 3, 5 or 6 h. System A function was analysed using \u03b1(methyl-amino)isobutyric acid (MeAIB) transcellular transport studies. Transporter (sodium coupled neutral amino acid transporter (SNAT1\/2)) expression was analysed at mRNA and protein level by Northern and Western blotting respectively. Localisation was carried out using immunocytochemistry. MeAIB transcellular transport was significantly (P &amp;lt; 0.05) increased by incubation of the cells in amino acid-depleted medium for 1 h, and longer incubation times caused further increases in the rate of transfer. However, the initial response was not accompanied by an increase in SNAT2 mRNA; this occurred only after 3 h and further increased for the rest of the 6-h incubation. Similarly, it took several hours for a significant increase in SNAT2 protein expression. In contrast, relocalisation of existing SNAT2 transporters occurred within 30 min of amino acid restriction and continued throughout the 6-h incubation. When the cells were incubated in medium with even lower amino acid levels (without non-essential plus 0.5 \u00d7 essential amino acids), SNAT2 mRNA levels showed further significant (P &amp;lt; 0.0001) up-regulation. However, incubation of cells in depleted medium for 6 h caused a significant (P = 0.014) decrease in the expression of SNAT1 mRNA. System L type amino acid transporter 2 (LAT2) expression was not changed by amino acid restriction, indicating that the responses seen in the system A transporters were not a general cell response. These data have shown that placental cells adapt in vitro to nutritional stress and have identified the physiological, biochemical and genomic mechanisms involved.<\/jats:p>","DOI":"10.1530\/rep.1.00808","type":"journal-article","created":{"date-parts":[[2006,5,3]],"date-time":"2006-05-03T12:59:14Z","timestamp":1146661154000},"page":"951-960","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":45,"title":["Expression and adaptive regulation of amino acid transport system A in a placental cell line under amino acid restriction"],"prefix":"10.1093","volume":"131","author":[{"given":"H N","family":"Jones","sequence":"first","affiliation":[{"name":"Maternal-Fetal Physiology, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK"},{"name":"Animal Breeding and Development, Scottish Agricultural College, Aberdeen AB21 9YA, UK"},{"name":"College of Life Science and Medicine, University of Aberdeen, Aberdeen AB25 2ZD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C J","family":"Ashworth","sequence":"additional","affiliation":[{"name":"Animal Breeding and Development, Scottish Agricultural College, Aberdeen AB21 9YA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K R","family":"Page","sequence":"additional","affiliation":[{"name":"College of Life Science and Medicine, University of Aberdeen, Aberdeen AB25 2ZD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H J","family":"McArdle","sequence":"additional","affiliation":[{"name":"Maternal-Fetal Physiology, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,5,1]]},"reference":[{"key":"2026010713035848400_BRACY-ETAL-1986","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1016\/S0021-9258(17)35969-0","volume":"261","year":"1986","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713035848400_CARSTENSEN-AND-LEICHTWEISS-1986","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1159\/000298911","volume":"22","year":"1986","journal-title":"Gynecologic and Obstetric Investigation"},{"key":"2026010713035848400_CRAMER-ETAL-2002","doi-asserted-by":"crossref","first-page":"C153","DOI":"10.1152\/ajpcell.2002.282.1.C153","volume":"282","year":"2002","journal-title":"American Journal of Physiology: Cell Physiology"},{"key":"2026010713035848400_DESFORGES-ETAL-2005","first-page":"C305","volume":"290","year":"2005","journal-title":"American Journal of Physiology"},{"key":"2026010713035848400_ENDERS-ETAL-1976","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1152\/ajplegacy.1976.230.3.706","volume":"230","year":"1976","journal-title":"American Journal of Physiology"},{"key":"2026010713035848400_FONG-ETAL-1990","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0005-2736(90)90281-R","volume":"1022","year":"1990","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026010713035848400_FRANCHI-GAZZOLA-ETAL-1999","doi-asserted-by":"crossref","first-page":"28922","DOI":"10.1074\/jbc.274.41.28922","volume":"274","year":"1999","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713035848400_GAMBLING-ETAL-2001","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1042\/bj3560883","volume":"356","year":"2001","journal-title":"Biochemical Journal"},{"key":"2026010713035848400_GAZZOLA-ETAL-1972","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/0005-2736(72)90097-1","volume":"266","year":"1972","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026010713035848400_HATANAKA-ETAL-2000","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0005-2736(00)00252-2","volume":"1467","year":"2000","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026010713035848400_HATANAKA-ETAL-2001","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S0014-5793(01)02848-4","volume":"505","year":"2001","journal-title":"FEBS Letters"},{"key":"2026010713035848400_HYDE-ETAL-2001","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1042\/bj3550563","volume":"355","year":"2001","journal-title":"Biochemical Journal"},{"key":"2026010713035848400_HYDE-ETAL-2002","doi-asserted-by":"crossref","first-page":"13628","DOI":"10.1074\/jbc.M108609200","volume":"277","year":"2002","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713035848400_JAYANTHI-ETAL-1995","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0005-2736(95)80024-A","volume":"1235","year":"1995","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026010713035848400_KARL-1995","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/jcp.1041650111","volume":"165","year":"1995","journal-title":"Journal of Cell Physiology"},{"key":"2026010713035848400_LENNON-ETAL-1996","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1006\/geno.1996.0177","volume":"33","year":"1996","journal-title":"Genomics"},{"key":"2026010713035848400_LING-ETAL-2001","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0005-2736(01)00310-8","volume":"1512","year":"2001","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026010713035848400_LIU-ETAL-1997","doi-asserted-by":"crossref","first-page":"C1596","DOI":"10.1152\/ajpcell.1997.273.5.C1596","volume":"273","year":"1997","journal-title":"American Journal of Physiology"},{"key":"2026010713035848400_MCDOWELL-ETAL-1998","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0014-5793(98)01508-7","volume":"441","year":"1998","journal-title":"FEBS Letters"},{"key":"2026010713035848400_MCGIVAN-AND-PASTOR-ANGLADA-1994","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1042\/bj2990321","volume":"299","year":"1994","journal-title":"Biochemical Journal"},{"key":"2026010713035848400_MOE-ETAL-1994","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/S0143-4004(05)80182-1","volume":"15","year":"1994","journal-title":"Placenta"},{"key":"2026010713035848400_NELSON-ETAL-2003","doi-asserted-by":"crossref","first-page":"C310","DOI":"10.1152\/ajpcell.00253.2002","volume":"284","year":"2003","journal-title":"American Journal of Physiology: Cell Physiology"},{"key":"2026010713035848400_NOVAK-AND-MATTHEWS-2003","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1203\/01.PDR.0000061542.06874.2A","volume":"53","year":"2003","journal-title":"Pediatric Research"},{"key":"2026010713035848400_OKAMOTO-ETAL-2002","doi-asserted-by":"crossref","first-page":"C196","DOI":"10.1152\/ajpcell.2002.282.1.C196","volume":"282","year":"2002","journal-title":"American Journal of Physiology"},{"key":"2026010713035848400_PALII-ETAL-2004","doi-asserted-by":"crossref","first-page":"3463","DOI":"10.1074\/jbc.M310483200","volume":"279","year":"2004","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713035848400_PINEDA-ETAL-1999","doi-asserted-by":"crossref","first-page":"19738","DOI":"10.1074\/jbc.274.28.19738","volume":"274","year":"1999","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713035848400_RITCHIE-AND-TAYLOR-2001","first-page":"716","volume":"356","year":"2001","journal-title":"Biochemical Journal"},{"key":"2026010713035848400_RUIZ-MONTASELL-ETAL-1994","doi-asserted-by":"crossref","first-page":"9569","DOI":"10.1073\/pnas.91.20.9569","volume":"91","year":"1994","journal-title":"PNAS"},{"key":"2026010713035848400_RUZYCKI-ETAL-1978","doi-asserted-by":"crossref","first-page":"C27","DOI":"10.1152\/ajpcell.1978.234.1.C27","volume":"234","year":"1978","journal-title":"American Journal of Physiology"},{"key":"2026010713035848400_SCHLEPPHORST-ETAL-1980","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/0002-9378(80)91136-9","volume":"137","year":"1980","journal-title":"American Journal of Obstetics and Gynecology"},{"key":"2026010713035848400_SCHNEIDER-ETAL-1987","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0143-4004(87)90017-8","volume":"8","year":"1987","journal-title":"Placenta"},{"key":"2026010713035848400_SIBLEY-ETAL-1997","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1113\/expphysiol.1997.sp004034","volume":"82","year":"1997","journal-title":"Experimental Physiology"},{"key":"2026010713035848400_SMITH-AND-DEPPER-1974","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1203\/00006450-197407000-00002","volume":"8","year":"1974","journal-title":"Pediatric Research"},{"key":"2026010713035848400_SMITH-ETAL-1973","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1152\/ajplegacy.1973.224.3.558","volume":"224","year":"1973","journal-title":"American Journal of Physiology"},{"key":"2026010713035848400_STEEL-ETAL-1982","doi-asserted-by":"crossref","first-page":"C46","DOI":"10.1152\/ajpcell.1982.243.1.C46","volume":"243","year":"1982","journal-title":"American Journal of Physiology"},{"key":"2026010713035848400_SUGAWARA-ETAL-2000A","doi-asserted-by":"crossref","first-page":"16473","DOI":"10.1074\/jbc.C000205200","volume":"275","year":"2000","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713035848400_SUGAWARA-ETAL-2000B","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0005-2736(00)00349-7","volume":"1509","year":"2000","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026010713035848400_TAKAHASHI-ETAL-2001","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1053\/plac.2001.0742","volume":"22","year":"2001","journal-title":"Placenta"},{"key":"2026010713035848400_TARNUZZER-ETAL-1990","doi-asserted-by":"crossref","first-page":"13914","DOI":"10.1016\/S0021-9258(18)77435-8","volume":"265","year":"1990","journal-title":"Journal of Biological Chemistry"},{"key":"2026010713035848400_UTOGUCHI-AND-AUDUS-2000","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0378-5173(99)00398-1","volume":"195","year":"2000","journal-title":"International Journal of Pharmacology"},{"key":"2026010713035848400_VARDHANA-AND-ILLSLEY-2002","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1053\/plac.2002.0857","volume":"23","year":"2002","journal-title":"Placenta"},{"key":"2026010713035848400_WANG-ETAL-2000","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1006\/bbrc.2000.3061","volume":"273","year":"2000","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2026010713035848400_WAY-ETAL-1998","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0143-4004(98)90065-0","volume":"19","year":"1998","journal-title":"Placenta"},{"key":"2026010713035848400_WHEELER-AND-YUDILEVICH-1989","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0005-2736(89)90123-5","volume":"978","year":"1989","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026010713035848400_WICE-ETAL-1990","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/0014-4827(90)90310-7","volume":"186","year":"1990","journal-title":"Experiments in Cell Research"},{"key":"2026010713035848400_YAO-ETAL-2000","doi-asserted-by":"crossref","first-page":"22790","DOI":"10.1074\/jbc.M002965200","volume":"275","year":"2000","journal-title":"Journal of Biological Chemistry"}],"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/131\/5\/1310951.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rep.bioscientifica.com\/downloadpdf\/journals\/rep\/131\/5\/1310951.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/131\/5\/951\/64608346\/1310951.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/131\/5\/951\/64608346\/1310951.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T18:16:17Z","timestamp":1767809777000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/131\/5\/951\/8252325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5,1]]},"references-count":46,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,5,1]]}},"URL":"https:\/\/doi.org\/10.1530\/rep.1.00808","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2006,5,1]]},"published":{"date-parts":[[2006,5,1]]}}}