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Cultured human granulosa-lutein cells were loaded with Fura-2 AM, and changes in [Ca2+]i concentrations within small areas of single cells were studied with a dynamic digital Ca2+ imaging system. Kepone, at concentrations of 0.2\u20132 nmol\/ml, consistently increased [Ca2+]i concentrations 2\u20136 times higher than baseline values within minutes of exposure. Methoxychlor at concentrations of 2.8\u2013280 nmol\/ml failed to alter [Ca2+]i levels consistently in cells from 10 patients. However, at 0.28 and 1.4 nmol\/ml, increases in [Ca2+]i concentrations could be elicited by methoxychlor. The isomer o,p-DDE at 3 nmol\/ml increased [Ca2+]i in granulosa cells of 11\/20 patients. Pertussis toxin treatment inhibited the [Ca2+]i increases induced by estradiol, p,p\u2032-DDE, o,p-DDE and methoxychlor, but not by Kepone or progesterone, indicating that Kepone and progesterone may act through an insensitive G protein-coupled receptor. The [Ca2+]i increases induced by Kepone also occurred in Ca2+-free medium, suggesting that [Ca2+]i mobilization occurred from the smooth endoplasmic reticulum. Thapsigargin and cyclopiazonic acid, two inhibitors of the endoplasmic reticulum Ca2+ pump, also stimulated [Ca2+]i increases but did not inhibit the Ca2+ response to all the pesticides. These results demonstrate that pesticides can have a rapid effect on human granulosa-lutein cells, and a nongenomic mechanism of action is suggested<\/jats:p>","DOI":"10.1530\/rep.1.00922","type":"journal-article","created":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T19:58:04Z","timestamp":1138823884000},"page":"299-310","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":7,"title":["Rapid effects of pesticides on human granulosa-lutein cells"],"prefix":"10.1093","volume":"131","author":[{"given":"Yanjing","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Reproductive Biology Division, McMaster University, Health Sciences Centre, 1200 Main Street West, Hamilton, Ontario, Canada, L8N 3Z5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warren G","family":"Foster","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Reproductive Biology Division, McMaster University, Health Sciences Centre, 1200 Main Street West, Hamilton, Ontario, Canada, L8N 3Z5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward V","family":"Younglai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Reproductive Biology Division, McMaster University, Health Sciences Centre, 1200 Main Street West, Hamilton, Ontario, Canada, L8N 3Z5"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,2,1]]},"reference":[{"key":"2026010713115320900_ANDERSEN-ETAL-2002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/taap.2001.9347","volume":"179","year":"2002","journal-title":"Toxicology and Applied Pharmacology"},{"key":"2026010713115320900_BERRIDGE-2004","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.bbamcr.2004.08.012","volume":"1742","year":"2004","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026010713115320900_BERRIDGE-2005","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev.physiol.67.040103.152647","volume":"67","year":"2005","journal-title":"Annual Review of Physiology"},{"key":"2026010713115320900_BERRIDGE-ETAL-2000","first-page":"11","volume":"1","year":"2000","journal-title":"Nature Reviews. 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