{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:20:54Z","timestamp":1791724854116,"version":"4.3.4"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The efficiency of cloning by somatic cell nuclear transfer (SCNT) is poor in livestock with ~5% of transferred cloned embryos developing to term. SCNT is associated with gross placental structural abnormalities. We aimed to identify defects in placental histology and gene expression in failing ovine cloned pregnancies to better understand why so many clones generated by SCNT die in utero. Placentomes from SCNT pregnancies (n = 9) and age matched, naturally mated controls (n = 20) were collected at two gestational age ranges (105\u2013134 days and 135\u2013154 days; term = 147 days). There was no effect of cloning on total placental weight. However, cloning reduced the number of placentomes at both gestational ages (105\u2013134 days: control 55.0 \u00b1 4.2, clone 44.7 \u00b1 8.0 and 135\u2013154 days: control 72.2 \u00b1 5.1, clone 36.6 \u00b1 5.1; P &amp;lt; 0.001) and increased the mean individual placentome weight (105\u2013134 days: control 10.6 \u00b1 1.3 g, clone 18.6 \u00b1 2.8 g and 135\u2013154 days: control 6.6 \u00b1 0.6 g, clone 7.0 \u00b1 2.0 g; P &amp;lt; 0.02). Placentomes from cloned pregnancies had a significant volume of shed trophoblast and fetal villous hemorrhage, absent in controls, at both gestational age ranges (P &amp;lt; 0.001) that was shown to be apoptotic by activated caspase-3 immunoreactivity. Consequently, the volume of intact trophoblast was reduced and the arithmetic mean barrier thickness of trophoblast through which exchange occurs was altered (P &amp;lt; 0.001) at both gestational age ranges in clones. In addition, cloning reduced placental expression of key genes in placental differentiation and function. Thus, cloning by SCNT results in both gross and microscopic placental abnormalities. We speculate that trophoblast apoptosis, shedding, and hemorrhage may be causal in fetal death in ovine clones.<\/jats:p>","DOI":"10.1530\/rep.1.01203","type":"journal-article","created":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T15:15:54Z","timestamp":1169565354000},"page":"243-255","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":45,"title":["Somatic cell nuclear transfer in the sheep induces placental defects that likely precede fetal demise"],"prefix":"10.1093","volume":"133","author":[{"given":"C J","family":"Fletcher","sequence":"first","affiliation":[{"name":"Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide SA 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C T","family":"Roberts","sequence":"additional","affiliation":[{"name":"Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide SA 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K M","family":"Hartwich","sequence":"additional","affiliation":[{"name":"Research Centre for the Early Origins of Adult Health, Discipline of Physiology, School of Molecular and Biomedical Science, University of Adelaide, Adelaide SA 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S K","family":"Walker","sequence":"additional","affiliation":[{"name":"South Australia Research and Development Institute, Turretfield Research Centre, Rosedale SA 5350, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I C","family":"McMillen","sequence":"additional","affiliation":[{"name":"Research Centre for the Early Origins of Adult Health, Discipline of Physiology, School of Molecular and Biomedical Science, University of Adelaide, Adelaide SA 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,1,1]]},"reference":[{"key":"2026010713135813600_ALEXANDER-1964A","first-page":"307","volume":"30","year":"1964","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010713135813600_ALEXANDER-1964B","first-page":"289","volume":"30","year":"1964","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010713135813600_BAKER-ETAL-1993","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0092-8674(05)80085-6","volume":"75","year":"1993","journal-title":"Cell"},{"key":"2026010713135813600_CHALLIS-AND-BROOKS-1989","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1210\/edrv-10-2-182","volume":"10","year":"1989","journal-title":"Endocrine Reviews"},{"key":"2026010713135813600_CHAVATTE-PALMER-ETAL-2002","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1095\/biolreprod66.6.1596","volume":"66","year":"2002","journal-title":"Biology of Reproduction"},{"key":"2026010713135813600_CHAVATTE-PALMER-ETAL-2004","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1089\/1536230041372274","volume":"6","year":"2004","journal-title":"Cloning and Stem Cells"},{"key":"2026010713135813600_CHRISTOFORI-ETAL-1994","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/369414a0","volume":"369","year":"1994","journal-title":"Nature"},{"key":"2026010713135813600_CIBELLI-ETAL-1998","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1126\/science.280.5367.1256","volume":"280","year":"1998","journal-title":"Science"},{"key":"2026010713135813600_CLARK-ETAL-1998","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1677\/joe.0.1590459","volume":"159","year":"1998","journal-title":"Journal of Endocrinology"},{"key":"2026010713135813600_CONSTANCIA-ETAL-2002","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1038\/nature00819","volume":"417","year":"2002","journal-title":"Nature"},{"key":"2026010713135813600_CONSTANCIA-ETAL-2005","doi-asserted-by":"crossref","first-page":"19219","DOI":"10.1073\/pnas.0504468103","volume":"102","year":"2005","journal-title":"PNAS"},{"key":"2026010713135813600_CONSTANT-ETAL-2006","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1095\/biolreprod.106.051581","volume":"75","year":"2006","journal-title":"Biology of Reproduction"},{"key":"2026010713135813600_CROSS-ETAL-1994","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1126\/science.7985020","volume":"266","year":"1994","journal-title":"Science"},{"key":"2026010713135813600_DAS-ETAL-2000","doi-asserted-by":"crossref","first-page":"R2252","DOI":"10.1152\/ajpregu.2000.279.6.R2252","volume":"279","year":"2000","journal-title":"American Journal of Physiology. 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