{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:44Z","timestamp":1784557184137,"version":"3.55.0"},"reference-count":60,"publisher":"Bioscientifica","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["vb.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,2,16]]},"abstract":"<jats:p>\n                    The placenta mediates the transport of nutrients, such as inorganic phosphate (P\n                    <jats:sub>i<\/jats:sub>\n                    ), between the maternal and fetal circulatory systems. The placenta itself also requires high levels of nutrient uptake as it develops to provide critical support for fetal development. This study aimed to determine placental P\n                    <jats:sub>i<\/jats:sub>\n                    transport mechanisms using\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    models. We observed that P\n                    <jats:sub>i<\/jats:sub>\n                    (P\n                    <jats:sup>33<\/jats:sup>\n                    ) uptake in BeWo cells is sodium dependent and that\n                    <jats:italic>SLC20A1\/Slc20a1<\/jats:italic>\n                    is the most highly expressed placental sodium-dependent transporter in mouse (microarray), human cell line (RT-PCR) and term placenta (RNA-seq), supporting that normal growth and maintenance of the mouse and human placenta requires\n                    <jats:italic>SLC20A1\/Slc20a1<\/jats:italic>\n                    .\n                    <jats:italic>Slc20a1<\/jats:italic>\n                    wild-type (\n                    <jats:italic>\n                      Slc20a1\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    ) and knockout (\n                    <jats:italic>\n                      Slc20a1\n                      <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    <\/jats:italic>\n                    ) mice were produced through timed intercrosses and displayed yolk sac angiogenesis failure as expected at E10.5. E9.5 tissues were analyzed to test whether placental morphogenesis requires\n                    <jats:italic>Slc20a1<\/jats:italic>\n                    . At E9.5, the developing placenta was reduced in size in\n                    <jats:italic>\n                      Slc20a1\n                      <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    <\/jats:italic>\n                    . Multiple structural abnormalities were also observed in the\n                    <jats:italic>\n                      Slc20a1\n                      <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    <\/jats:italic>\n                    chorioallantois. We determined that monocarboxylate transporter 1 protein (MCT1\n                    <jats:italic>+<\/jats:italic>\n                    ) cells were reduced in developing\n                    <jats:italic>\n                      Slc20a1\n                      <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    <\/jats:italic>\n                    placenta, confirming that\n                    <jats:italic>Slc20a1<\/jats:italic>\n                    loss reduced trophoblast syncytiotrophoblast 1 (SynT-I) coverage. Next, we examined the cell type-specific\n                    <jats:italic>Slc20a1<\/jats:italic>\n                    expression and SynT molecular pathways\n                    <jats:italic>in silico<\/jats:italic>\n                    and identified\n                    <jats:italic>Notch\/Wnt<\/jats:italic>\n                    as a pathway of interest that regulates trophoblast differentiation. We further observed that specific trophoblast lineages express\n                    <jats:italic>Notch\/Wnt<\/jats:italic>\n                    genes that associate with endothelial cell tip-and-stalk cell markers. In conclusion, our findings support that\n                    <jats:italic>Slc20a1<\/jats:italic>\n                    mediates the symport of P\n                    <jats:sub>i<\/jats:sub>\n                    into SynT cells, providing critical support for their differentiation and angiogenic mimicry function at the developing maternal\n                    <jats:italic>\u2013<\/jats:italic>\n                    fetal interface.\n                  <\/jats:p>","DOI":"10.1530\/vb-22-0018","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T13:29:27Z","timestamp":1676554167000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-12","source":"Crossref","is-referenced-by-count":3,"title":["SLC20a1\/PiT-1 is required for chorioallantoic placental morphogenesis"],"prefix":"10.1530","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5353-9665","authenticated-orcid":true,"given":"Ana","family":"Correia-Branco","sequence":"first","affiliation":[{"name":"Mother Infant Research Institute, Tufts Medical Center, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0327-757X","authenticated-orcid":true,"given":"Ariel","family":"Mei","sequence":"additional","affiliation":[{"name":"Mother Infant Research Institute, Tufts Medical Center, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6004-7001","authenticated-orcid":true,"given":"Sreehari","family":"Pillai","sequence":"additional","affiliation":[{"name":"Mother Infant Research Institute, Tufts Medical Center, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9483-7271","authenticated-orcid":true,"given":"Nirmala","family":"Jayaraman","sequence":"additional","affiliation":[{"name":"Mother Infant Research Institute, Tufts Medical Center, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5582-9647","authenticated-orcid":true,"given":"Radhika","family":"Sharma","sequence":"additional","affiliation":[{"name":"Mother Infant Research Institute, Tufts Medical Center, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6065-9398","authenticated-orcid":true,"given":"Alison G","family":"Paquette","sequence":"additional","affiliation":[{"name":"University of Washington, Department of Pediatrics, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveen K","family":"Neradugomma","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5734-8508","authenticated-orcid":true,"given":"Ciara","family":"Benson","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9058-0245","authenticated-orcid":true,"given":"Nicholas W","family":"Chavkin","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingcheng","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3682-156X","authenticated-orcid":true,"given":"Mary C","family":"Wallingford","sequence":"additional","affiliation":[{"name":"Mother Infant Research Institute, Tufts Medical Center, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Advances in Chronic Kidney Disease","first-page":"63","article-title":"Phosphate transporters in renal, gastrointestinal, and other tissues","volume":"18","author":"Forster","year":"2011","unstructured":"Forster IHernando NSorribas V & Werner A. 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