{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T14:11:43Z","timestamp":1769091103357,"version":"3.49.0"},"reference-count":58,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,9,1]]},"abstract":"<jats:sec>\n<jats:title>Introduction<\/jats:title>\n<jats:p>Thoracic perivascular adipose tissue (tPVAT) has a phenotype resembling brown AT. Dysfunctional tPVAT appears to be linked to vascular dysfunction.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>We evaluated uncoupling protein 1 (UCP1) expression by Western blot, oxidative stress by measuring lipid peroxidation, the antioxidant capacity by HPLC and spectrophotometry, and mitochondrial respiration by high-resolution respirometry (HRR) in tPVAT, compared to inguinal white AT (iWAT), obtained from non-diabetic (NDM) and streptozocin-induced diabetic (STZ-DM) mice. Mitochondrial respiration was assessed by HRR using protocol 1: complex I and II oxidative phosphorylation (OXPHOS) and protocol 2: fatty acid oxidation (FAO) OXPHOS. OXPHOS capacity in tPVAT was also evaluated after UCP1 inhibition by guanosine 5'-diphosphate (GDP).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>UCP1 expression was higher in tPVAT when compared with iWAT in both NDM and STZ-DM mice. The malondialdehyde concentration was elevated in tPVAT from STZ-DM compared to NDM mice. Glutathione peroxidase and reductase activities, as well as reduced glutathione levels, were not different between tPVAT from NDM and STZ-DM mice but were lower compared to iWAT of STZ-DM mice. OXPHOS capacity of tPVAT was significantly decreased after UCP1 inhibition by GDP in protocol 1. While there were no differences in the OXPHOS capacity between NDM and STZ-DM mice in protocol 1, it was increased in STZ-DM compared to NDM mice in protocol 2. Moreover, complex II- and FAO-linked respiration were elevated in STZ-DM mice under UCP1 inhibition.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Pharmacological therapies could be targeted to modulate UCP1 activity with a significant impact in the uncoupling of mitochondrial bioenergetics in tPVAT.<\/jats:p>\n<\/jats:sec>","DOI":"10.1530\/joe-21-0446","type":"journal-article","created":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T08:11:02Z","timestamp":1656490262000},"page":"169-184","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":3,"title":["Mitochondrial respiration in thoracic perivascular adipose tissue of diabetic mice"],"prefix":"10.1530","volume":"254","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2235-2237","authenticated-orcid":true,"given":"Aryane Cruz Oliveira","family":"Pinho","sequence":"first","affiliation":[{"name":"Center for Neuroscience and Cell Biology (CNC), Faculty of Medicine, University of Coimbra, Rua Larga, Coimbra, Portugal"},{"name":"Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Cal\u00e7ada Martim de Freitas, Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9292-5961","authenticated-orcid":true,"given":"Diana","family":"Santos","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology (CNC), Faculty of Medicine, University of Coimbra, Rua Larga, Coimbra, Portugal"},{"name":"Institute for Interdisciplinary Research, University of Coimbra, Casa Costa Alem\u00e3o, Rua Dom Francisco de Lemos, Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8106-7308","authenticated-orcid":true,"given":"In\u00eas","family":"Baldeiras","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology (CNC), Faculty of Medicine, University of Coimbra, Rua Larga, Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8467-3840","authenticated-orcid":true,"given":"Ana","family":"Burgeiro","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology (CNC), Faculty of Medicine, University of Coimbra, Rua Larga, Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8467-3840","authenticated-orcid":true,"given":"Emelindo C","family":"Leal","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology (CNC), Faculty of Medicine, University of Coimbra, Rua Larga, Coimbra, Portugal"},{"name":"Institute for Interdisciplinary Research, University of Coimbra, Casa Costa Alem\u00e3o, Rua Dom Francisco de Lemos, Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1748-9861","authenticated-orcid":true,"given":"Eugenia","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Center for Neuroscience and Cell Biology (CNC), Faculty of Medicine, University of Coimbra, Rua Larga, Coimbra, Portugal"},{"name":"Institute for Interdisciplinary Research, University of Coimbra, Casa Costa Alem\u00e3o, Rua Dom Francisco de Lemos, Coimbra, Portugal"},{"name":"APDP-Portuguese Diabetes Association, Lisbon, Portugal"}]}],"member":"416","reference":[{"key":"bib1","series-title":"Heart Failure Reviews","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1007\/s10741-014-9457-4","article-title":"Mitochondrial oxidative metabolism and uncoupling proteins in the failing heart","volume":"20","author":"Akhmedov","year":"2015","unstructured":"Akhmedov ATRybin VMar\u00edn-Garc\u00eda J2015Mitochondrial oxidative metabolism and uncoupling proteins in the failing heart. 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