{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:28:05Z","timestamp":1767860885289,"version":"3.49.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sertoli cells are responsible for regulating a wide range of processes that lead to the differentiation of male germ cells into spermatozoa. Intracellular pH (pHi) is an important parameter in cell physiology regulating namely cell metabolism and differentiation. However, pHi regulation mechanisms in Sertoli cells have not yet been systematically elucidated. In this work, pHi was determined in primary cultures of human Sertoli cells. Sertoli cells were exposed to weak acids, which caused a rapid acidification of the intracellular milieu. pHi then recovered by a mechanism that was shown to be particularly sensitive to the presence of the inhibitor DIDS (4,4\u2032-diisothiocyanostilbene disulfonic acid). In the presence of amiloride and PSA (picrylsulfonic acid), pHi recovery was also significantly affected. These results indicate that, in the experimental conditions used, pHi is regulated by the action of an Na+-driven HCO3\u2212\/Cl\u2212 exchanger and an Na+\/HCO3\u2212 co-transporter and also by the action of the Na+\/H+ exchanger. On the other hand, pHi recovery was only slightly affected by concanamycin A, suggesting that V-Type ATPases do not have a relevant action on pHi regulation in human Sertoli cells, and was independent of the presence of bumetanide, suggesting that the inhibition of the Na+\/K+\/Cl\u2212 co-transporter does not affect pHi recovery, not even indirectly via the shift of ionic gradients. Finally, pHi was shown to be sensitive to the removal of external Cl\u2212, but not of Na+ or K+, evidencing the presence of a membrane Cl\u2212-dependent base extruder, namely the Na+-independent HCO3\u2212\/Cl\u2212 exchanger, and its role on pHi maintenance on these cells.<\/jats:p>","DOI":"10.1530\/rep-08-0363","type":"journal-article","created":{"date-parts":[[2008,11,21]],"date-time":"2008-11-21T21:26:35Z","timestamp":1227302795000},"page":"353-359","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":48,"title":["Intracellular pH regulation in human Sertoli cells: role of membrane transporters"],"prefix":"10.1093","volume":"137","author":[{"given":"P F","family":"Oliveira","sequence":"first","affiliation":[{"name":"UMIB Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto, L. Prof. Abel Salazar, 2, 4099-003 Porto, Portugal 2Laborat\u00f3rio de Biologia Celular ICBAS-UP, 4099-003 Porto, Portugal 3Departamento de Gen\u00e9tica FM-UP, 4200-319 Porto, Portugal 4REQUIMTE Departamento de Qu\u00edmica, FCT-UNL, 2829-516 Caparica, Portugal 5Laborat\u00f3rio de Fisiologia Geral ICBAS-UP, 4099-003 Porto, Portugal"}]},{"given":"M","family":"Sousa","sequence":"additional","affiliation":[{"name":"UMIB Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto, L. Prof. Abel Salazar, 2, 4099-003 Porto, Portugal 2Laborat\u00f3rio de Biologia Celular ICBAS-UP, 4099-003 Porto, Portugal 3Departamento de Gen\u00e9tica FM-UP, 4200-319 Porto, Portugal 4REQUIMTE Departamento de Qu\u00edmica, FCT-UNL, 2829-516 Caparica, Portugal 5Laborat\u00f3rio de Fisiologia Geral ICBAS-UP, 4099-003 Porto, Portugal"}]},{"given":"A","family":"Barros","sequence":"additional","affiliation":[{"name":"UMIB Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto, L. Prof. Abel Salazar, 2, 4099-003 Porto, Portugal 2Laborat\u00f3rio de Biologia Celular ICBAS-UP, 4099-003 Porto, Portugal 3Departamento de Gen\u00e9tica FM-UP, 4200-319 Porto, Portugal 4REQUIMTE Departamento de Qu\u00edmica, FCT-UNL, 2829-516 Caparica, Portugal 5Laborat\u00f3rio de Fisiologia Geral ICBAS-UP, 4099-003 Porto, Portugal"}]},{"given":"T","family":"Moura","sequence":"additional","affiliation":[{"name":"UMIB Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto, L. Prof. Abel Salazar, 2, 4099-003 Porto, Portugal 2Laborat\u00f3rio de Biologia Celular ICBAS-UP, 4099-003 Porto, Portugal 3Departamento de Gen\u00e9tica FM-UP, 4200-319 Porto, Portugal 4REQUIMTE Departamento de Qu\u00edmica, FCT-UNL, 2829-516 Caparica, Portugal 5Laborat\u00f3rio de Fisiologia Geral ICBAS-UP, 4099-003 Porto, Portugal"}]},{"given":"A","family":"Rebelo da Costa","sequence":"additional","affiliation":[{"name":"UMIB Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto, L. Prof. Abel Salazar, 2, 4099-003 Porto, Portugal 2Laborat\u00f3rio de Biologia Celular ICBAS-UP, 4099-003 Porto, Portugal 3Departamento de Gen\u00e9tica FM-UP, 4200-319 Porto, Portugal 4REQUIMTE Departamento de Qu\u00edmica, FCT-UNL, 2829-516 Caparica, Portugal 5Laborat\u00f3rio de Fisiologia Geral ICBAS-UP, 4099-003 Porto, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2009,2,1]]},"reference":[{"key":"2026010712494100800_bib1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1242\/jeb.196.1.319","article-title":"Role of the invertebrate electrogenic 2Na+\/1H+ antiporter in monovalent and divalent cation transport","volume":"196","author":"Ahearn","year":"1994","journal-title":"Journal of Experimental Biology"},{"key":"2026010712494100800_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1086\/316643","article-title":"Ion transport processes of crustacean epithelial cells","volume":"72","author":"Ahearn","year":"1999","journal-title":"Physiological and Biochemical Zoology"},{"key":"2026010712494100800_bib3","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1242\/jeb.02014","article-title":"The V-type H+ ATPase: molecular structure and function, physiological roles and regulation","volume":"209","author":"Beyenbach","year":"2006","journal-title":"Journal of Experimental Biology"},{"key":"2026010712494100800_bib4","first-page":"176","article-title":"Sodium-coupled bicarbonate transporters","volume":"2","author":"Boron","year":"2001","journal-title":"Journal of the Pancreas"},{"key":"2026010712494100800_bib5","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1152\/advan.00045.2004","article-title":"Regulation of intracellular pH","volume":"28","author":"Boron","year":"2004","journal-title":"Advances in Physiology Education"},{"key":"2026010712494100800_bib6","first-page":"445","article-title":"The electrogenic Na\/HCO3 cotransporter","volume":"109","author":"Boron","year":"1997","journal-title":"Wiener Klinische Wochenschrift"},{"key":"2026010712494100800_bib7","doi-asserted-by":"crossref","first-page":"F1","DOI":"10.1152\/ajprenal.00340.2006","article-title":"New insights into the regulation of V-ATPase-dependent proton secretion","volume":"292","author":"Breton","year":"2007","journal-title":"American Journal of Physiology. Renal Physiology"},{"key":"2026010712494100800_bib8","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1530\/rep.0.1240633","article-title":"Marked extension of proliferation of rat Sertoli cells in culture using recombinant human FSH","volume":"124","author":"Buzzard","year":"2002","journal-title":"Reproduction"},{"key":"2026010712494100800_bib9","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1210\/endo.132.3.8382599","article-title":"Calcium-mediated intracellular acidification and activation of Na(+)-H+ exchange in adrenal glomerulosa cells stimulated with potassium","volume":"132","author":"Conlin","year":"1993","journal-title":"Endocrinology"},{"key":"2026010712494100800_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1074\/jbc.275.1.1","article-title":"The expanding family of eucaryotic Na(+)\/H(+) exchangers","volume":"275","author":"Counillon","year":"2000","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712494100800_bib11","first-page":"G842","article-title":"Amiloride and analogues inhibit Na(+)-H+ exchange and cell proliferation in AR42J pancreatic cell line","volume":"259","author":"Delvaux","year":"1990","journal-title":"American Journal of Physiology"},{"key":"2026010712494100800_bib12","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1242\/jeb.200.2.247","article-title":"The key role of the H+V-ATPase in acid\u2013base balance and Na+ transport processes in frog skin","volume":"200","author":"Ehrenfeld","year":"1997","journal-title":"Journal of Experimental Biology"},{"key":"2026010712494100800_bib13","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1432-1033.1988.tb14055.x","article-title":"The regulation of the intracellular pH in cells from vertebrates","volume":"174","author":"Frelin","year":"1988","journal-title":"European Journal of Biochemistry"},{"key":"2026010712494100800_bib14","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1152\/physrev.00011.2004","article-title":"Molecular physiology and pathophysiology of electroneutral cation\u2013chloride cotransporters","volume":"85","author":"Gamba","year":"2005","journal-title":"Physiological Reviews"},{"key":"2026010712494100800_bib15","doi-asserted-by":"crossref","first-page":"12525","DOI":"10.1073\/pnas.92.26.12525","article-title":"Basolateral membrane Na+\/H+ exchange enhances HCO3\u2212 absorption in rat medullary thick ascending limb: evidence for functional coupling between basolateral and apical membrane Na+\/H+ exchangers","volume":"92","author":"Good","year":"1995","journal-title":"PNAS"},{"key":"2026010712494100800_bib16","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1210\/endo.136.5.7720654","article-title":"Androgens rapidly increase the cytosolic calcium concentration in Sertoli cells","volume":"136","author":"Gorczynska","year":"1995","journal-title":"Endocrinology"},{"key":"2026010712494100800_bib17","doi-asserted-by":"crossref","first-page":"8778","DOI":"10.1016\/S0021-9258(19)84448-4","article-title":"Na+\/HCO3-co-transport in basolateral membrane vesicles isolated from rabbit renal cortex","volume":"261","author":"Grassl","year":"1986","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712494100800_bib18","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1095\/biolreprod52.2.211","article-title":"Interactions between germ cells and Sertoli cells in the testis","volume":"52","author":"Griswold","year":"1995","journal-title":"Biology of Reproduction"},{"key":"2026010712494100800_bib19","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1006\/scdb.1998.0203","article-title":"The central role of Sertoli cells in spermatogenesis","volume":"9","author":"Griswold","year":"1998","journal-title":"Seminars in Cell and Developmental Biology"},{"key":"2026010712494100800_bib20","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1242\/jeb.172.1.289","article-title":"Energization of sodium absorption by the H(+)-ATPase pump in mitochondria-rich cells of frog skin","volume":"172","author":"Harvey","year":"1992","journal-title":"Journal of Experimental Biology"},{"key":"2026010712494100800_bib21","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/0014-4835(88)90091-7","article-title":"Characterization of Cl\u2212\/HCO3\u2212 exchange in cultured bovine pigmented ciliary epithelium","volume":"47","author":"Helbig","year":"1988","journal-title":"Experimental Eye Research"},{"key":"2026010712494100800_bib22","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1095\/biolreprod64.6.1699","article-title":"Distribution of the vacuolar H+ ATPase along the rat and human male reproductive tract","volume":"64","author":"Herak-Kramberger","year":"2001","journal-title":"Biology of Reproduction"},{"key":"2026010712494100800_bib23","doi-asserted-by":"crossref","first-page":"40544","DOI":"10.1074\/jbc.M207345200","article-title":"Concanamycin A, the specific inhibitor of V-ATPases, binds to the V(o) subunit c","volume":"277","author":"Huss","year":"2002","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712494100800_bib24","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1085\/jgp.58.2.211","article-title":"Chemical modification of membranes: II. Permeation paths for sulfhydryl agents","volume":"58","author":"Knauf","year":"1971","journal-title":"Journal of General Physiology"},{"key":"2026010712494100800_bib25","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1055\/s-2002-35426","article-title":"Rapid effect of testosterone on rat Sertoli cell membrane potential. Relationship with K+ATP channels","volume":"34","author":"Von Ledebur","year":"2002","journal-title":"Hormone and Metabolic Research"},{"key":"2026010712494100800_bib26","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1095\/biolreprod63.3.736","article-title":"Rapid androgen actions on calcium signaling in rat sertoli cells and two human prostatic cell lines: similar biphasic responses between 1 picomolar and 100 nanomolar concentrations","volume":"63","author":"Lyng","year":"2000","journal-title":"Biology of Reproduction"},{"key":"2026010712494100800_bib27","doi-asserted-by":"crossref","first-page":"7486","DOI":"10.1016\/S0021-9258(18)47592-8","article-title":"Selective inhibition of sodium-linked and sodium-independent bicarbonate\/chloride antiport in Vero cells","volume":"262","author":"Madshus","year":"1987","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712494100800_bib28","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1095\/biolreprod52.3.658","article-title":"Isolation and culture of Sertoli cells from the testes of adult Siberian hamsters: analysis of proteins synthesized and secreted by Sertoli cells cultured from hamsters raised in a long or a short photoperiod","volume":"52","author":"Majumdar","year":"1995","journal-title":"Biology of Reproduction"},{"key":"2026010712494100800_bib29","doi-asserted-by":"crossref","first-page":"C1015","DOI":"10.1152\/ajpcell.1993.265.4.C1015","article-title":"Vacuolar-type H(+)-ATPases are functionally expressed in plasma membranes of human tumor cells","volume":"265","author":"Martinez-Zaguilan","year":"1993","journal-title":"American Journal of Physiology"},{"key":"2026010712494100800_bib30","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1242\/jeb.193.1.97","article-title":"Ion transport in the intestine of Anguilla Anguilla: gradients and translocators","volume":"193","author":"Marvao","year":"1994","journal-title":"Journal of Experimental Biology"},{"key":"2026010712494100800_bib31","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1210\/rp.57.1.149","article-title":"Identification of specific sites of hormonal regulation in spermatogenesis in rats, monkeys, and man","volume":"57","author":"McLachlan","year":"2002","journal-title":"Recent Progress in Hormone Research"},{"key":"2026010712494100800_bib32","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1210\/er.2003-0022","article-title":"Sertoli\u2013Sertoli and Sertoli\u2013germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis","volume":"25","author":"Mruk","year":"2004","journal-title":"Endocrinology Reviews"},{"key":"2026010712494100800_bib33","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1247\/csf.18.139","article-title":"Folimycin (concanamycin A), a specific inhibitor of V-ATPase, blocks intracellular translocation of the glycoprotein of vesicular stomatitis virus before arrival to the Golgi apparatus","volume":"18","author":"Muroi","year":"1993","journal-title":"Cell Structure and Function"},{"key":"2026010712494100800_bib34","doi-asserted-by":"crossref","first-page":"C177","DOI":"10.1152\/ajpcell.1987.253.2.C177","article-title":"Characteristics and functions of Na\u2013K\u2013Cl cotransport in epithelial tissues","volume":"253","author":"O'Grady","year":"1987","journal-title":"American Journal of Physiology"},{"key":"2026010712494100800_bib35","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.cbpb.2004.07.004","article-title":"H+-ATPase of crude homogenate of the outer mantle epithelium of Anodonta cygnea","volume":"139","author":"Oliveira","year":"2004","journal-title":"Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology"},{"key":"2026010712494100800_bib36","doi-asserted-by":"crossref","first-page":"22373","DOI":"10.1074\/jbc.272.36.22373","article-title":"Na+\/H+ exchangers of mammalian cells","volume":"272","author":"Orlowski","year":"1997","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712494100800_bib37","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1476-5381.1990.tb15783.x","article-title":"Effects of inhibitors of protein kinase C and Na+\/H+ exchange on alpha 1-adrenoceptor-mediated inotropic responses in the rat left ventricular papillary muscle","volume":"100","author":"Otani","year":"1990","journal-title":"British Journal of Pharmacology"},{"key":"2026010712494100800_bib38","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1172\/JCI8553","article-title":"Failure of spermatogenesis in mouse lines deficient in the Na(+)-K(+)-2Cl(\u2212) cotransporter","volume":"105","author":"Pace","year":"2000","journal-title":"Journal of Clinical Investigation"},{"key":"2026010712494100800_bib39","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1016\/S0021-9258(19)84545-3","article-title":"Growth factors activate the bumetanide-sensitive Na+\/K+\/Cl\u2212 cotransport in hamster fibroblasts","volume":"261","author":"Paris","year":"1986","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712494100800_bib40","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S1095-6433(99)00069-0","article-title":"Identification of a V-type proton pump in the outer mantle epithelium of Anodonta cygnea","volume":"123","author":"Rebelo da Costa","year":"1999","journal-title":"Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology"},{"key":"2026010712494100800_bib41","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1152\/physrev.1981.61.2.296","article-title":"Intracellular pH","volume":"61","author":"Roos","year":"1981","journal-title":"Physiological Reviews"},{"key":"2026010712494100800_bib42","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/264073a0","article-title":"Role of choloride transport in regulation of intracellular pH","volume":"264","author":"Russell","year":"1976","journal-title":"Nature"},{"key":"2026010712494100800_bib43","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1530\/eje.0.1470425","article-title":"Rapid signal transduction in Sertoli cells","volume":"147","author":"Silva","year":"2002","journal-title":"European Journal of Endocrinology"},{"key":"2026010712494100800_bib44","doi-asserted-by":"crossref","first-page":"7645","DOI":"10.1016\/S0021-9258(19)39163-X","article-title":"A vacuolar type H(+)-ATPase regulates cytoplasmic pH in murine macrophages","volume":"265","author":"Swallow","year":"1990","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712494100800_bib45","first-page":"311","article-title":"Intracellularly trapped pH indicators","volume":"40","author":"Thomas","year":"1986","journal-title":"Society of General Physiologists Series"},{"key":"2026010712494100800_bib46","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/BF00593663","article-title":"The composition of fluid collected by micropuncture and catheterization from the seminiferous tubules and rete testis of rats","volume":"318","author":"Tuck","year":"1970","journal-title":"Pfl\u00fcgers Archiv"},{"key":"2026010712494100800_bib47","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1111\/j.1399-3089.2005.00235.x","article-title":"Method for evaluating quality of cultured neonatal pig Sertoli cells","volume":"12","author":"Valdes-Gonzalez","year":"2005","journal-title":"Xenotransplantation"},{"key":"2026010712494100800_bib48","doi-asserted-by":"crossref","first-page":"19925","DOI":"10.1016\/S0021-9258(17)32108-7","article-title":"Na(+)-K(+)-Cl\u2212 cotransporter of brain capillary endothelial cells. Properties and regulation by endothelins, hyperosmolar solutions, calyculin A, and interleukin-1","volume":"269","author":"Vigne","year":"1994","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712494100800_bib49","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1530\/rep.1.00358","article-title":"FSH and testosterone signaling in Sertoli cells","volume":"130","author":"Walker","year":"2005","journal-title":"Reproduction"},{"key":"2026010712494100800_bib50","doi-asserted-by":"crossref","first-page":"35486","DOI":"10.1074\/jbc.C000456200","article-title":"The Na+-driven Cl\u2212\/HCO3\u2212 exchanger. Cloning, tissue distribution, and functional characterization","volume":"275","author":"Wang","year":"2000","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712494100800_bib51","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1210\/endo-96-3-618","article-title":"Rat sertoli cells: a rapid method for obtaining viable cells","volume":"96","author":"Welsh","year":"1975","journal-title":"Endocrinology"}],"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/137\/2\/353.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/137\/2\/353\/64531221\/353.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/137\/2\/353\/64531221\/353.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T17:52:04Z","timestamp":1767808324000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/137\/2\/353\/8243032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2,1]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2,1]]}},"URL":"https:\/\/doi.org\/10.1530\/rep-08-0363","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2009,2,1]]},"published":{"date-parts":[[2009,2,1]]}}}