{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:35:15Z","timestamp":1767861315180,"version":"3.49.0"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"vor","delay-in-days":3776,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/deed.en_GB"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>During follicle development, oocytes secrete factors that influence the development of granulosa and cumulus cells (CCs). In response to oocyte and somatic cell signals, CCs produce extracellular matrix (ECM) molecules resulting in cumulus expansion, which is essential for ovulation, fertilisation, and is predictive of oocyte quality. The cumulus ECM is largely made up of hyaluronan (HA), TNF-stimulated gene-6 (TSG-6, also known as TNFAIP6), pentraxin-3 (PTX3), and the heavy chains (HCs) of serum-derived inter-\u03b1-inhibitor proteins. In contrast to other in vivo models where modified expansion impairs fertility, the cumulus mass of C1galt1 Mutants, which have oocyte-specific deletion of core 1-derived O-glycans, is modified without impairing fertility. In this report, we used C1galt1 Mutant (C1galt1FF:ZP3Cre) and Control (C1galt1FF) mice to investigate how cumulus expansion is affected by oocyte-specific deletion of core 1-derived O-glycans without adversely affecting oocyte quality. Mutant cumulus\u2013oocyte complexes (COCs) are smaller than Controls, with fewer CCs. Interestingly, the CCs in Mutant mice are functionally normal as each cell produced normal levels of the ECM molecules HA, TSG-6, and PTX3. However, HC levels were elevated in Mutant COCs. These data reveal that oocyte glycoproteins carrying core 1-derived O-glycans have a regulatory role in COC development. In addition, our study of Controls indicates that a functional COC can form provided all essential components are present above a minimum threshold level, and thus some variation in ECM composition does not adversely affect oocyte development, ovulation or fertilisation. These data have important implications for IVF and the use of cumulus expansion as a criterion for oocyte assessment.<\/jats:p>","DOI":"10.1530\/rep-14-0503","type":"journal-article","created":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T22:21:29Z","timestamp":1428531689000},"page":"533-543","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":17,"title":["Molecular analysis of the cumulus matrix: insights from mice with\n                    <i>O<\/i>\n                    -glycan-deficient oocytes"],"prefix":"10.1093","volume":"149","author":[{"given":"Panayiota","family":"Ploutarchou","sequence":"first","affiliation":[]},{"given":"Pedro","family":"Melo","sequence":"additional","affiliation":[]},{"given":"Anthony J","family":"Day","sequence":"additional","affiliation":[{"name":"Nuffield Department of Obstetrics and Gynaecology Women's Centre, Level 3, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK 1 University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK 2 Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK"},{"name":"Faculty of Life Sciences Women's Centre, Level 3, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK 1 University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK 2 Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK"},{"name":"Wellcome Trust Centre for Cell-Matrix Research Women's Centre, Level 3, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK 1 University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK 2 Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK"}]},{"given":"Caroline M","family":"Milner","sequence":"additional","affiliation":[{"name":"Nuffield Department of Obstetrics and Gynaecology Women's Centre, Level 3, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK 1 University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK 2 Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK"},{"name":"Faculty of Life Sciences Women's Centre, Level 3, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK 1 University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK 2 Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK"},{"name":"Wellcome Trust Centre for Cell-Matrix Research Women's Centre, Level 3, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK 1 University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK 2 Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK"}]},{"given":"Suzannah A","family":"Williams","sequence":"additional","affiliation":[]}],"member":"286","published-online":{"date-parts":[[2015,5,1]]},"reference":[{"key":"2026010712400073000_bib1","doi-asserted-by":"crossref","first-page":"30481","DOI":"10.1074\/jbc.M114.568154","article-title":"Incorporation of pentraxin 3 into hyaluronan matrices is tightly regulated and promotes matrix cross-linking","volume":"289","author":"Baranova","year":"2014","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1095\/biolreprod.111.098103","article-title":"Complex N-glycans are essential, but core 1 and 2 mucin O-glycans, O-fucose glycans, and NOTCH1 are dispensable, for mammalian spermatogenesis","volume":"86","author":"Batista","year":"2012","journal-title":"Biology of Reproduction"},{"key":"2026010712400073000_bib3","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1095\/biolreprod65.1.301","article-title":"TSG-6 is concentrated in the extracellular matrix of mouse cumulus oocyte complexes through hyaluronan and inter-\u03b1-inhibitor binding","volume":"65","author":"Carrette","year":"2001","journal-title":"Biology of Reproduction"},{"key":"2026010712400073000_bib4","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1002\/mrd.1080340114","article-title":"Functional significance of cumulus expansion in the mouse: roles for the preovulatory synthesis of hyaluronic acid within the cumulus mass","volume":"34","author":"Chen","year":"1993","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712400073000_bib5","doi-asserted-by":"crossref","first-page":"19409","DOI":"10.1074\/jbc.271.32.19409","article-title":"Covalent linkage between proteins of the inter-\u03b1-inhibitor family and hyaluronic acid is mediated by a factor produced by granulosa cells","volume":"271","author":"Chen","year":"1996","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib6","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1172\/JCI70291","article-title":"Prenatal retinoid deficiency leads to airway hyperresponsiveness in adult mice","volume":"124","author":"Chen","year":"2014","journal-title":"Journal of Clinical Investigation"},{"key":"2026010712400073000_bib7","article-title":"Oocyte glycoproteins regulate the form and function of the follicle basal lamina and theca cells","author":"Christensen","year":"2014","journal-title":"Developmental Biology"},{"key":"2026010712400073000_bib8","doi-asserted-by":"crossref","first-page":"6511","DOI":"10.1167\/iovs.11-7909","article-title":"Mapping the differential distribution of glycosaminoglycans in the adult human retina, choroid, and sclera","volume":"52","author":"Clark","year":"2011","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"2026010712400073000_bib9","doi-asserted-by":"crossref","first-page":"4585","DOI":"10.1074\/jbc.R100036200","article-title":"Hyaluronan-binding proteins: tying up the giant","volume":"277","author":"Day","year":"2002","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib10","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1016\/j.fertnstert.2007.10.061","article-title":"Leukemia inhibitory factor induces cumulus expansion in immature human and mouse oocytes and improves mouse two-cell rate and delivery rates when it is present during mouse in vitro oocyte maturation","volume":"90","author":"De Matos","year":"2008","journal-title":"Fertility and Sterility"},{"key":"2026010712400073000_bib11","doi-asserted-by":"crossref","first-page":"2798","DOI":"10.1210\/en.2005-0098","article-title":"Role of oocyte-secreted growth differentiation factor 9 in the regulation of mouse cumulus expansion","volume":"146","author":"Dragovic","year":"2005","journal-title":"Endocrinology"},{"key":"2026010712400073000_bib12","first-page":"77","article-title":"IVM results are comparable and may have advantages over standard IVF","volume":"6","author":"Ellenbogen","year":"2014","journal-title":"Facts, Views & Vision in ObGyn"},{"key":"2026010712400073000_bib13","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1006\/abbi.1996.9793","article-title":"Coding sequence of a hyaluronan synthase homologue expressed during expansion of the mouse cumulus\u2013oocyte complex","volume":"337","author":"Fulop","year":"1997","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"2026010712400073000_bib14","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0378-1119(97)00459-9","article-title":"Coding sequence, exon\u2013intron structure and chromosomal localization of murine TNF-stimulated gene 6 that is specifically expressed by expanding cumulus cell\u2013oocyte complexes","volume":"202","author":"Fulop","year":"1997","journal-title":"Gene"},{"key":"2026010712400073000_bib15","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1242\/dev.00422","article-title":"Impaired cumulus mucification and female sterility in tumor necrosis factor-induced protein-6 deficient mice","volume":"130","author":"Fulop","year":"2003","journal-title":"Development"},{"key":"2026010712400073000_bib16","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1146\/annurev.immunol.23.021704.115756","article-title":"Pentraxins at the crossroads between innate immunity, inflammation, matrix deposition, and female fertility","volume":"23","author":"Garlanda","year":"2005","journal-title":"Annual Review of Immunology"},{"key":"2026010712400073000_bib17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1093\/humupd\/dmm040","article-title":"Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality","volume":"14","author":"Gilchrist","year":"2008","journal-title":"Human Reproduction Update"},{"key":"2026010712400073000_bib18","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1096\/fj.14-253757","article-title":"Oocytes lacking O-glycans alter follicle development and increase fertility by increasing follicle FSH sensitivity, decreasing apoptosis, and modifying GDF9:BMP15 expression","volume":"29","author":"Grasa","year":"2014","journal-title":"FASEB Journal"},{"issue":"Suppl 4","key":"2026010712400073000_bib19","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1093\/humrep\/13.suppl_4.117","article-title":"Ovarian markers of implantation potential in assisted reproduction","volume":"13","author":"Gregory","year":"1998","journal-title":"Human Reproduction"},{"key":"2026010712400073000_bib20","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1095\/biolreprod58.3.705","article-title":"The ovarian blood follicle barrier is both charge- and size-selective in mice","volume":"58","author":"Hess","year":"1998","journal-title":"Biology of Reproduction"},{"key":"2026010712400073000_bib21","doi-asserted-by":"crossref","first-page":"5619","DOI":"10.1074\/jbc.M113.542357","article-title":"A refined model for the TSG-6 link module in complex with hyaluronan: use of defined oligosaccharides to probe structure and function","volume":"289","author":"Higman","year":"2014","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib22","first-page":"1017","article-title":"Successful assisted reproductive technology: the beauty of one","volume":"100","author":"Hogue","year":"2002","journal-title":"Obstetrics and Gynecology"},{"key":"2026010712400073000_bib23","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.matbio.2011.05.002","article-title":"Implication of the oligomeric state of the N-terminal PTX3 domain in cumulus matrix assembly","volume":"30","author":"Ievoli","year":"2011","journal-title":"Matrix Biology"},{"key":"2026010712400073000_bib24","doi-asserted-by":"crossref","first-page":"17681","DOI":"10.1074\/jbc.M109.085639","article-title":"The angiogenic inhibitor long pentraxin PTX3 forms an asymmetric octamer with two binding sites for FGF2","volume":"285","author":"Inforzato","year":"2010","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib25","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1530\/rep.0.1230097","article-title":"Composition and morphology of the follicular basal lamina during atresia of bovine antral follicles","volume":"123","author":"Irving-Rodgers","year":"2002","journal-title":"Reproduction"},{"key":"2026010712400073000_bib26","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/S0093-691X(00)00387-3","article-title":"Effects of oocyte quality, oxygen tension, embryo density, cumulus cells and energy substrates on cleavage and morula\/blastocyst formation of bovine embryos","volume":"54","author":"Khurana","year":"2000","journal-title":"Theriogenology"},{"key":"2026010712400073000_bib27","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1530\/rep.1.01074","article-title":"TGF-\u03b2 superfamily members and ovarian follicle development","volume":"132","author":"Knight","year":"2006","journal-title":"Reproduction"},{"key":"2026010712400073000_bib58","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/S0092-8674(00)80151-8","article-title":"Solution structure of the link module: a hyaluronan-binding domain involved in extracellular matrix stability and cell migration","volume":"86","author":"Kohda","year":"1996","journal-title":"Cell"},{"key":"2026010712400073000_bib28","doi-asserted-by":"crossref","first-page":"e74602","DOI":"10.1371\/journal.pone.0074602","article-title":"Involvement of the serine protease inhibitor, SERPINE2, and the urokinase plasminogen activator in cumulus expansion and oocyte maturation","volume":"8","author":"Lu","year":"2013","journal-title":"PLoS ONE"},{"key":"2026010712400073000_bib29","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1093\/oxfordjournals.humrep.a138718","article-title":"An immunohistochemical study of the vascularization of the human Graafian follicle","volume":"9","author":"McClure","year":"1994","journal-title":"Human Reproduction"},{"key":"2026010712400073000_bib30","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/0022-4731(87)90329-3","article-title":"Gonadotrophins, fecundity genes and ovarian follicular function","volume":"27","author":"McNatty","year":"1987","journal-title":"Journal of Steroid Biochemistry"},{"key":"2026010712400073000_bib31","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1242\/jcs.00407","article-title":"TSG-6: a multifunctional protein associated with inflammation","volume":"116","author":"Milner","year":"2003","journal-title":"Journal of Cell Science"},{"key":"2026010712400073000_bib32","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1042\/BST0340446","article-title":"TSG-6: a pluripotent inflammatory mediator?","volume":"34","author":"Milner","year":"2006","journal-title":"Biochemical Society Transactions"},{"key":"2026010712400073000_bib33","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1006\/abbi.2001.2552","article-title":"Two distinct populations of tumor necrosis factor-stimulated gene-6 protein in the extracellular matrix of expanded mouse cumulus cell\u2013oocyte complexes","volume":"394","author":"Mukhopadhyay","year":"2001","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"2026010712400073000_bib34","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1093\/humrep\/deu035","article-title":"Combined blockade of angiotensin II type 1 receptor and activation of peroxisome proliferator-activated receptor-\u03b3 by telmisartan effectively inhibits vascularization and growth of murine endometriosis-like lesions","volume":"29","author":"Nenicu","year":"2014","journal-title":"Human Reproduction"},{"key":"2026010712400073000_bib35","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/S0015-0282(99)00290-3","article-title":"Prediction of the rates of fertilization, cleavage, and pregnancy success by cumulus\u2013coronal morphology in an in vitro fertilization program","volume":"72","author":"Ng","year":"1999","journal-title":"Fertility and Sterility"},{"key":"2026010712400073000_bib36","doi-asserted-by":"crossref","first-page":"3656","DOI":"10.1182\/blood-2014-04-572107","article-title":"Podoplanin requires sialylated O-glycans for stable expression on lymphatic endothelial cells and for interaction with platelets","volume":"124","author":"Pan","year":"2014","journal-title":"Blood"},{"key":"2026010712400073000_bib37","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1126\/science.1092463","article-title":"EGF-like growth factors as mediators of LH action in the ovulatory follicle","volume":"303","author":"Park","year":"2004","journal-title":"Science"},{"key":"2026010712400073000_bib38","doi-asserted-by":"crossref","first-page":"E776","DOI":"10.1073\/pnas.1218020110","article-title":"Growth differentiation factor 9:bone morphogenetic protein 15 heterodimers are potent regulators of ovarian functions","volume":"110","author":"Peng","year":"2013","journal-title":"PNAS"},{"key":"2026010712400073000_bib39","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/0012-1606(87)90192-8","article-title":"Oocyte-specific expression and developmental regulation of ZP3, the sperm receptor of the mouse zona pellucida","volume":"121","author":"Philpott","year":"1987","journal-title":"Developmental Biology"},{"key":"2026010712400073000_bib40","doi-asserted-by":"crossref","first-page":"25674","DOI":"10.1074\/jbc.M501332200","article-title":"Characterization of complexes formed between TSG-6 and inter-\u03b1-inhibitor that act as intermediates in the covalent transfer of heavy chains onto hyaluronan","volume":"280","author":"Rugg","year":"2005","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib41","doi-asserted-by":"crossref","first-page":"13840","DOI":"10.1016\/S0021-9258(18)80077-1","article-title":"Synthesis and accumulation of hyaluronic acid and proteoglycans in the mouse cumulus cell\u2013oocyte complex during follicle-stimulating hormone-induced mucification","volume":"264","author":"Salustri","year":"1989","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib42","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1242\/dev.01056","article-title":"PTX3 plays a key role in the organization of the cumulus oophorus extracellular matrix and in in vivo fertilization","volume":"131","author":"Salustri","year":"2004","journal-title":"Development"},{"key":"2026010712400073000_bib43","doi-asserted-by":"crossref","first-page":"11936","DOI":"10.1074\/jbc.M409016200","article-title":"The TSG-6 and I\u03b1I interaction promotes a transesterification cleaving the protein-glycosaminoglycan-protein (PGP) cross-link","volume":"280","author":"Sanggaard","year":"2005","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib44","doi-asserted-by":"crossref","first-page":"18530","DOI":"10.1074\/jbc.M800874200","article-title":"The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2","volume":"283","author":"Sanggaard","year":"2008","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib45","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1006\/bbrc.2001.4475","article-title":"Impaired fertility in female mice lacking urinary trypsin inhibitor","volume":"281","author":"Sato","year":"2001","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2026010712400073000_bib46","doi-asserted-by":"crossref","first-page":"30161","DOI":"10.1074\/jbc.M703738200","article-title":"PTX3 interacts with inter-\u03b1-trypsin inhibitor: implications for hyaluronan organization and cumulus oophorus expansion","volume":"282","author":"Scarchilli","year":"2007","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712400073000_bib47","doi-asserted-by":"crossref","first-page":"9920","DOI":"10.1128\/MCB.24.22.9920-9929.2004","article-title":"Inactivation of the Mgat1 gene in oocytes impairs oogenesis, but embryos lacking complex and hybrid N-glycans develop and implant","volume":"24","author":"Shi","year":"2004","journal-title":"Molecular and Cellular Biology"},{"key":"2026010712400073000_bib48","doi-asserted-by":"crossref","first-page":"a005199","DOI":"10.1101\/cshperspect.a005199","article-title":"Golgi glycosylation","volume":"3","author":"Stanley","year":"2011","journal-title":"Cold Spring Harbor Perspectives in Biology"},{"key":"2026010712400073000_bib49","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.ydbio.2004.08.020","article-title":"Synergistic roles of BMP15 and GDF9 in the development and function of the oocyte\u2013cumulus cell complex in mice: genetic evidence for an oocyte-granulosa cell regulatory loop","volume":"276","author":"Su","year":"2004","journal-title":"Developmental Biology"},{"key":"2026010712400073000_bib50","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1002\/mrd.10102","article-title":"Minireview: Functions of the cumulus oophorus during oocyte maturation, ovulation, and fertilization","volume":"61","author":"Tanghe","year":"2002","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712400073000_bib51","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/ncomms1874","article-title":"O-glycosylation modulates integrin and FGF signalling by influencing the secretion of basement membrane components","volume":"3","author":"Tian","year":"2012","journal-title":"Nature Communications"},{"key":"2026010712400073000_bib52","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.theriogenology.2013.05.009","article-title":"Importance of the GDF9 signaling pathway on cumulus cell expansion and oocyte competency in sheep","volume":"80","author":"Varnosfaderani Sh","year":"2013","journal-title":"Theriogenology"},{"key":"2026010712400073000_bib53","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1096\/fj.07-101709","article-title":"Mouse fertility is enhanced by oocyte-specific loss of core 1-derived O-glycans","volume":"22","author":"Williams","year":"2008","journal-title":"FASEB Journal"},{"key":"2026010712400073000_bib54","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1242\/jcs.004291","article-title":"Fertilization in mouse does not require terminal galactose or N-acetylglucosamine on the zona pellucida glycans","volume":"120","author":"Williams","year":"2007","journal-title":"Journal of Cell Science"},{"key":"2026010712400073000_bib55","doi-asserted-by":"crossref","first-page":"9204","DOI":"10.1073\/pnas.1003110107","article-title":"Core 1-derived O-glycans are essential E-selectin ligands on neutrophils","volume":"107","author":"Yago","year":"2010","journal-title":"PNAS"},{"key":"2026010712400073000_bib56","doi-asserted-by":"crossref","first-page":"4114","DOI":"10.1210\/endo.141.11.7784","article-title":"Expression of tumor necrosis factor-stimulated gene-6 in the rat ovary in response to an ovulatory dose of gonadotropin","volume":"141","author":"Yoshioka","year":"2000","journal-title":"Endocrinology"},{"key":"2026010712400073000_bib57","doi-asserted-by":"crossref","first-page":"7693","DOI":"10.1074\/jbc.C000899200","article-title":"Defect in SHAP\u2013hyaluronan complex causes severe female infertility. A study by inactivation of the bikunin gene in mice","volume":"276","author":"Zhuo","year":"2001","journal-title":"Journal of Biological Chemistry"}],"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/149\/5\/533.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/149\/5\/533\/64132146\/533.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/149\/5\/533\/64132146\/533.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T17:40:16Z","timestamp":1767807616000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/149\/5\/533\/8236012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,1]]},"references-count":58,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5,1]]}},"URL":"https:\/\/doi.org\/10.1530\/rep-14-0503","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2015,5,1]]},"published":{"date-parts":[[2015,5,1]]}}}