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In experiment 1, there were no significant differences in rates of blastocyst formation among embryos cultured in our standard medium (DMEM\/F-12), which contained &amp;gt;16\u200amM glucose, and those cultured in a minimal-glucose embryo culture medium (&amp;lt;1\u200amM; Global medium, GB), with either 0 added glucose for the first 5 days, then 20\u200amM (0-20) or 20\u200amM for the entire culture period (20-20). In experiment 2, there were no significant differences in the rates of blastocyst development (31\u201346%) for embryos cultured in four glucose treatments in GB (0-10, 0-20, 5-10, or 5-20). Blastocysts were evaluated by immunofluorescence for lineage-specific markers. All cells stained positively for POU5F1. An inner cluster of cells was identified that included presumptive primitive endoderm cells (GATA6-positive) and presumptive epiblast (EPI) cells. The 5-20 treatment resulted in a significantly lower number of presumptive EPI-lineage cells than the 0-20 treatment did. GATA6-positive cells appeared to be allocated to the primitive endoderm independent of the formation of an inner cell mass, as was previously hypothesized for equine embryos. These data demonstrate that equine blastocyst development is not dependent on high glucose concentrations during early culture; rather, environmental glucose may affect cell allocation. They also present the first analysis of cell lineage allocation in in vitro-fertilized equine blastocysts. These findings expand our understanding of the factors that affect embryo development in the horse.<\/jats:p>","DOI":"10.1530\/rep-14-0662","type":"journal-article","created":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T00:45:40Z","timestamp":1428453940000},"page":"31-41","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":36,"title":["Cell lineage allocation in equine blastocysts produced\n                    <i>in vitro<\/i>\n                    under varying glucose concentrations"],"prefix":"10.1093","volume":"150","author":[{"given":"Young-Ho","family":"Choi","sequence":"first","affiliation":[]},{"given":"Pablo","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology and Pharmacology College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4466, USA 1 University of California, Davis, Davis, California 95616, USA 2 Do\u00f1a Pilar Embriones SRL, Lincoln, Argentina"},{"name":"Department of Animal Science College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4466, USA 1 University of California, Davis, Davis, California 95616, USA 2 Do\u00f1a Pilar Embriones SRL, Lincoln, Argentina"},{"name":"Laboratorio de Reproducci\u00f3n Equina Prof. Robert M. Kenney College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4466, USA 1 University of California, Davis, Davis, California 95616, USA 2 Do\u00f1a Pilar Embriones SRL, Lincoln, Argentina"}]},{"given":"Isabel C","family":"Velez","sequence":"additional","affiliation":[]},{"given":"B","family":"Mac\u00edas-Garc\u00eda","sequence":"additional","affiliation":[]},{"given":"Fernando L","family":"Riera","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology and Pharmacology College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4466, USA 1 University of California, Davis, Davis, California 95616, USA 2 Do\u00f1a Pilar Embriones SRL, Lincoln, Argentina"},{"name":"Department of Animal Science College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4466, USA 1 University of California, Davis, Davis, California 95616, USA 2 Do\u00f1a Pilar Embriones SRL, Lincoln, Argentina"},{"name":"Laboratorio de Reproducci\u00f3n Equina Prof. Robert M. Kenney College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4466, USA 1 University of California, Davis, Davis, California 95616, USA 2 Do\u00f1a Pilar Embriones SRL, Lincoln, Argentina"}]},{"given":"Katrin","family":"Hinrichs","sequence":"additional","affiliation":[]}],"member":"286","published-online":{"date-parts":[[2015,7,1]]},"reference":[{"key":"2026010712421459300_bib1","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abr\u00e0moff","year":"2004","journal-title":"Biophotonics International"},{"key":"2026010712421459300_bib2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1530\/jrf.0.0990015","article-title":"Quantitative analysis of morphological modifications of day 6.5 horse embryos after cryopreservation: differential effects on inner cell mass and trophoblast cells","volume":"99","author":"Bruyas","year":"1993","journal-title":"Journal of Reproduction and 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