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Biol."],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Microfluidic systems have advantages such as a high throughput, small reaction volume, and precise control of the cellular position and environment. These advantages have allowed microfluidics to be widely used in several fields of synthetic biology in recent years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this article, we reviewed the microfluidic\u2010based methods for synthetic biology from two aspects: the construction of synthetic gene circuits and the analysis of synthetic gene systems. We used some examples to illuminate the progresses and challenges in the steps of synthetic gene circuits construction and approaches of gene expression analysis with microfluidic systems.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Comparing to traditional methods, microfluidic tools promise great advantages in the synthetic genetic circuit building and analysis process. Moreover, new microfluidic systems together with the mathematical modeling of synthetic circuits or consortiums are desirable to perform complex genetic circuit construction and understand the natural gene regulation in cells and population interactions better.<\/jats:p><\/jats:sec>","DOI":"10.15302\/j-qb-021-0235","type":"journal-article","created":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T03:59:36Z","timestamp":1612151976000},"page":"47-60","source":"Crossref","is-referenced-by-count":6,"title":["Microfluidic approaches for synthetic gene circuits\u2019 construction and analysis"],"prefix":"10.1002","volume":"9","author":[{"given":"Fengyu","family":"Zhang","sequence":"first","affiliation":[{"name":"<!--1--> The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics School of Physics Peking University Beijing 100871 China"},{"name":"<!--2--> School of Life Sciences and Peking\u2010Tsinghua Center for Life Sciences Peking University Beijing 100871 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhong","family":"Sun","sequence":"additional","affiliation":[{"name":"<!--1--> The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics School of Physics Peking University Beijing 100871 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunxiong","family":"Luo","sequence":"additional","affiliation":[{"name":"<!--1--> The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics School of Physics Peking University Beijing 100871 China"},{"name":"<!--3--> Center for Quantitative Biology Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China"},{"name":"<!--4--> Wenzhou Institute University of Chinese Academy of Sciences Wenzhou 325001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,3]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05058"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2857"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708321105"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2017.12.004"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1039\/B806803H"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1310254110"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11641"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac504611t"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-019-0415-8"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.201600121"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq092"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12896\u2010018\u20100439\u20109"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm4014"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2010.08.002"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.5b00062"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3873"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958\u20101669(99)00003\u20108"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5330.1259"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.8.2745"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep23937"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/mi10060387"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7LC00552K"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.6b00011"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.5776007"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7LC00576H"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat5005"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8LC00582F"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b05754"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.4926616"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201002505\u20100"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc41196b"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127115"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8LC01397G"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1913003117"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11641"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1510328112"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/intbio\/zyz007"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048275"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100042"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1703379114"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep42200"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41378\u2010019\u20100099\u20105"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41378\u2010019\u20100109\u20107"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14622"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8LC00470F"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1039\/C9LC01248F"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8LC00193F"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b04099"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1039\/C3LC51332G"},{"key":"e_1_2_7_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010019\u20100533\u20100"},{"key":"e_1_2_7_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2857"},{"key":"e_1_2_7_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2625"},{"key":"e_1_2_7_54_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1070919"},{"key":"e_1_2_7_55_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1913003117"},{"key":"e_1_2_7_56_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1308265110"},{"key":"e_1_2_7_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100620\u20107"},{"key":"e_1_2_7_58_2","doi-asserted-by":"publishe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