{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T08:18:47Z","timestamp":1768983527216,"version":"3.49.0"},"reference-count":71,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0507504"],"award-info":[{"award-number":["2018YFA0507504"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["61773025"],"award-info":[{"award-number":["61773025"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070666"],"award-info":[{"award-number":["32070666"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Quant. Biol."],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The concept of biomolecular condensate was put forward recently to emphasize the ability of certain cellular compartments to concentrate molecules and comprise proteins and nucleic acids with specific biological functions, from ribosome genesis to RNA splicing. Due to their unique role in biological processes, it is crucial to investigate their compositions, which is a primary determinant of condensate properties.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Since a wide range of macromolecules comprise biomolecular condensates, it is necessary for researchers to investigate them using high\u2010throughput methodologies while low\u2010throughput experiments are not efficient enough. These high\u2010throughput methods usually purify interacting protein libraries from condensates before being scanned in mass spectrometry. It is possible to extract organelles as a whole for specific condensates for further analysis, however, most condensates do not have a distinguishable marker or are sensitive to shear force to be extracted as a whole. Affinity tagging allows a comprehensive view of interacting proteins of target molecule yet only proteins with strong bonds may be pulled down. Proximity labeling serves as a complementary method to label more dynamic proteins with weaker interactions, increasing sensitivity while decreasing specificity. Image\u2010based fluorescent screening takes another path by scanning images automatically to illustrate the condensing state of biomolecules within membraneless organelles, which is a unique feature unlike the previous mass spectrometry\u2010based methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This review presents a rough glimpse into high\u2010throughput methodologies for biomolecular condensate investigation to encourage usage of bioinformatic tools by researchers in relevant fields.<\/jats:p><\/jats:sec>","DOI":"10.15302\/j-qb-021-0264","type":"journal-article","created":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T00:55:02Z","timestamp":1627606502000},"page":"255-266","source":"Crossref","is-referenced-by-count":3,"title":["High\u2010throughput experimental methods for investigating biomolecular condensates"],"prefix":"10.1002","volume":"9","author":[{"given":"Taoyu","family":"Chen","sequence":"first","affiliation":[{"name":"<!--1--> Department of Biomedical Informatics School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Lei","sequence":"additional","affiliation":[{"name":"<!--1--> Department of Biomedical Informatics School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minglei","family":"Shi","sequence":"additional","affiliation":[{"name":"<!--2--> MOE Key Laboratory of Bioinformatics Bioinformatics Division and Center for Synthetic &amp; Systems Biology TNLIST School of Medicine Tsinghua University Beijing 100084 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Li","sequence":"additional","affiliation":[{"name":"<!--1--> Department of Biomedical Informatics School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9]]},"reference":[{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.7"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12985"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101464\u20100"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.08.011"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000638"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2184"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.12.035"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172046"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/ncrna5040050"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010cellbio\u2010100913\u2010013325"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.47098"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21249437"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201600144"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124619"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01511"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz847"},{"key":"e_1_2_11_18_2","first-page":"D360","article-title":"PhaSePro: the database of proteins driving liquid\u2010liquid phase separation.","volume":"48","author":"M\u00e9sz\u00e1ros P.","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz778"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz1027"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.05.076"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.31486"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2020.11.003"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03207"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.06.051"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.09.003"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u201062703\u2010977\u20102_14"},{"key":"e_1_2_11_28_2","unstructured":"Green M. R. and Sambrook J. (2012) Molecular Cloning: A Laboratory Manual 4th Ed. New York City: Cold Spring Harbor Laboratory Press"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.529578"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109258(18)86949\u20106"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010002\u20101158\u20106"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10529\u2010011\u20100592\u2010x"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E15\u201003\u20100136"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600718"},{"key":"e_1_2_11_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147\u2010619X(88)90037\u20106"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M600346\u2010MCP200"},{"key":"e_1_2_11_37_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.16903.1"},{"key":"e_1_2_11_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20104159.2010.07027.x"},{"key":"e_1_2_11_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2375"},{"key":"e_1_2_11_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2017.10.015"},{"key":"e_1_2_11_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3179"},{"key":"e_1_2_11_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.12.032"},{"key":"e_1_2_11_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022\u20101759(89)90104\u2010X"},{"key":"e_1_2_11_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022\u20101759(91)90399\u2010Z"},{"key":"e_1_2_11_45_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201910207"},{"key":"e_1_2_11_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14023"},{"key":"e_1_2_11_47_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1602426"},{"key":"e_1_2_11_48_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1515623112"},{"key":"e_1_2_11_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/74487"},{"key":"e_1_2_11_50_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201112098"},{"key":"e_1_2_11_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.12.020"},{"key":"e_1_2_11_52_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E15\u201012\u20100844"},{"key":"e_1_2_11_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4601"},{"key":"e_1_2_11_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4201"},{"key":"e_1_2_11_55_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1919528117"},{"key":"e_1_2_11_56_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000646"},{"key":"e_1_2_11_57_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000687"},{"key":"e_1_2_11_58_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201601071"},{"key":"e_1_2_11_59_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.206854"},{"key":"e_1_2_11_60_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1820737116"},{"key":"e_1_2_11_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3067-8_8"},{"key":"e_1_2_11_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4595"},{"key":"e_1_2_11_63_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb2125s101"},{"key":"e_1_2_11_64_2","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20102253\u20106_11"},{"key":"e_1_2_11_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010020\u201001010\u20105"},{"key":"e_1_2_11_66_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2006617117"},{"key":"e_1_2_11_67_2","doi-asserted-by":"crossref","unstructured":"Li Y. Liu S. Cao L. Luo Y. Du H. Li S. and You F. (2021) CBRPP: a new RNA\u2010centric method to study RNA\u2010protein interactions. RNA Biol. doi:10.1080\/15476286.2021.1873620","DOI":"10.1101\/2020.04.09.033290"},{"key":"e_1_2_11_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010020\u20100866\u20100"},{"key":"e_1_2_11_69_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b05303"},{"key":"e_1_2_11_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.10.036"},{"key":"e_1_2_11_71_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010020\u20100826\u20108"},{"key":"e_1_2_11_72_2","unstructured":"Lyon A. S. Peeples W. B. and Rosen M. K. (2021) A framework for understanding the functions of biomolecular condensates across scales. Nat. Rev. Mol. Cell Biol. doi:10.1038\/s41580\u2010020\u201000303\u2010z"}],"container-title":["Quantitative Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.15302\/J-QB-021-0264","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T09:52:35Z","timestamp":1735897955000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.15302\/J-QB-021-0264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":71,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.15302\/J-QB-021-0264"],"URL":"https:\/\/doi.org\/10.15302\/j-qb-021-0264","archive":["Portico"],"relation":{},"ISSN":["2095-4689","2095-4697"],"issn-type":[{"value":"2095-4689","type":"print"},{"value":"2095-4697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9]]}}}