{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:40:51Z","timestamp":1790970051605,"version":"4.1.0"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Cilia are microtubule based organelles that project from cells. Cilia are found on almost every cell type of the human body and numerous diseases, collectively termed ciliopathies, are associated with defects in cilia, including respiratory infections, male infertility, situs inversus, polycystic kidney disease, retinal degeneration, and Bardet-Biedl Syndrome. Here we show that Illumina-based whole-genome transcriptome analysis in the biflagellate green alga Chlamydomonas reinhardtii identifies 1850 genes up-regulated during ciliogenesis, 4392 genes down-regulated, and 4548 genes with no change in expression during ciliogenesis. We examined four genes up-regulated and not previously known to be involved with cilia (ZMYND10, NXN, GLOD4, SPATA4) by knockdown of the human orthologs in human retinal pigment epithelial cells (hTERT-RPE1) cells to ask whether they are involved in cilia-related processes that include cilia assembly, cilia length control, basal body\/centriole numbers, and the distance between basal bodies\/centrioles. All of the genes have cilia-related phenotypes and, surprisingly, our data show that knockdown of GLOD4 and SPATA4 also affects the cell cycle. These results demonstrate that whole-genome transcriptome analysis during ciliogenesis is a powerful tool to gain insight into the molecular mechanism by which centrosomes and cilia are assembled.<\/jats:p>","DOI":"10.1534\/g3.113.006338","type":"journal-article","created":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T03:08:00Z","timestamp":1366686480000},"page":"979-991","source":"Crossref","is-referenced-by-count":68,"title":["Identification of Cilia Genes That Affect Cell-Cycle Progression Using Whole-Genome Transcriptome Analysis in <i>Chlamydomonas reinhardtti<\/i>"],"prefix":"10.1093","volume":"3","author":[{"given":"Alison J","family":"Albee","sequence":"first","affiliation":[{"name":"Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan L","family":"Kwan","sequence":"additional","affiliation":[{"name":"Center for Genomic Sciences and System Biology, Washington University, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huawen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Granas","sequence":"additional","affiliation":[{"name":"Center for Genomic Sciences and System Biology, Washington University, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary D","family":"Stormo","sequence":"additional","affiliation":[{"name":"Center for Genomic Sciences and System Biology, Washington University, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan K","family":"Dutcher","sequence":"additional","affiliation":[{"name":"Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"},{"name":"Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,6,1]]},"reference":[{"key":"2021041813034125600_bib1","volume-title":"Cilia and Human Disease in eLS","author":"Albee","year":"2012"},{"key":"2021041813034125600_bib2","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0092-8674(04)00412-X","article-title":"Decoding cilia function: defining specialized genes required for compartmentalized cilia biogenesis.","volume":"117","author":"Avidor-Reiss","year":"2004","journal-title":"Cell"},{"key":"2021041813034125600_bib3","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1083\/jcb.200808082","article-title":"hPOC5 is a centrin-binding protein required for assembly of full-length centrioles.","volume":"185","author":"Azimzadeh","year":"2009","journal-title":"J. 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