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In Caenorhabditis elegans, the class I histone deacetylase family member hda-1, a component of the nucleosome remodeling and deacetylation complex, has been shown to control cell proliferation. We recovered hda-1 in an RNA interference screen for genes involved in the morphogenesis of the egg-laying system. We found that hda-1 mutants have abnormal vulva morphology and vulval-uterine connections (i.e., no uterine-seam cell). We characterized the vulval defects by using cell fate-specific markers and found that hda-1 is necessary for the specification of all seven vulval cell types. The analysis of the vulval-uterine connection defect revealed that hda-1 is required for the differentiation of the gonadal anchor cell (AC), which in turn induces ventral uterine granddaughters to adopt \u03c0 fates, leading to the formation of the uterine-seam cell. Consistent with these results, hda-1 is expressed in the vulva and AC. A search for hda-1 target genes revealed that fos-1 (fos proto-oncogene family) acts downstream of hda-1 in vulval cells, whereas egl-43 (evi1 proto-oncogene family) and nhr-67 (tailless homolog, NHR family) mediate hda-1 function in the AC. Furthermore, we showed that AC expression of hda-1 plays a crucial role in the regulation of the lin-12\/Notch ligand lag-2 to specify \u03c0 cell fates. These results demonstrate the pivotal role of hda-1 in the formation of the vulva and the vulval-uterine connection. Given that hda-1 homologs are conserved across the phyla, our findings are likely to provide a better understanding of HDAC1 function in development and disease.<\/jats:p>","DOI":"10.1534\/g3.113.006999","type":"journal-article","created":{"date-parts":[[2013,8,7]],"date-time":"2013-08-07T21:10:01Z","timestamp":1375909801000},"page":"1363-1374","source":"Crossref","is-referenced-by-count":21,"title":["<i>Caenorhabditis elegans<\/i>Histone Deacetylase<i>hda-1<\/i>Is Required for Morphogenesis of the Vulva and LIN-12\/Notch-Mediated Specification of Uterine Cell Fates"],"prefix":"10.1093","volume":"3","author":[{"given":"Ayush Vasant","family":"Ranawade","sequence":"first","affiliation":[{"name":"Department of Biology, McMaster University, Hamilton, ON L8S 4K1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Cumbo","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, Hamilton, ON L8S 4K1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhagwati P","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, Hamilton, ON L8S 4K1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,8,1]]},"reference":[{"key":"2021041813052649900_bib1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","article-title":"The genetics of Caenorhabditis elegans.","volume":"77","author":"Brenner","year":"1974","journal-title":"Genetics"},{"key":"2021041813052649900_bib2","doi-asserted-by":"crossref","first-page":"7197","DOI":"10.1093\/emboj\/20.24.7197","article-title":"A POP-1 repressor complex restricts inappropriate cell type-specific gene transcription during Caenorhabditis elegans embryogenesis.","volume":"20","author":"Calvo","year":"2001","journal-title":"EMBO J."},{"key":"2021041813052649900_bib3","doi-asserted-by":"crossref","unstructured":"Cui, M., and M. 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