{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T01:20:59Z","timestamp":1782955259896,"version":"3.54.5"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We describe the results of a systematic search for a class of hitherto-overlooked chemical-genetic interactions in the Saccharomyces cerevisiae genome, which exists between a detrimental genetic mutation and a chemical\/drug that can ameliorate, rather than exacerbate, that detriment. We refer to this type of interaction as \u201cchemical suppression.\u201d Our work was driven by the hypothesis that genome instability in a certain class of mutants could be alleviated by mild replication inhibition using chemicals\/drugs. We queried a collection of conditionally lethal, i.e., temperature-sensitive, alleles representing 40% of the yeast essential genes for those mutants whose growth defect can be suppressed by hydroxyurea (HU), known as a potent DNA replication inhibitor, at the restrictive temperature. Unexpectedly, we identified a number of mutants defective in diverse cellular pathways other than DNA replication. Here we report that HU suppresses selected mutants defective in the kinetochore-microtubule attachment pathway during mitotic chromosome segregation. HU also suppresses an ero1-1 mutant defective for a thiol oxidase of the endoplasmic reticulum by providing oxidation equivalents. Finally, we report that HU suppresses an erg26-1 mutant defective for a C-3 sterol dehydrogenase through regulating iron homeostasis and in turn impacting ergosterol biosynthesis. We further demonstrate that cells carrying the erg26-1 mutation show an increased rate of mitochondrial DNA loss and delayed G1 to S phase transition. We conclude that systematic gathering of a compendium of \u201cchemical suppression\u201d of yeast mutants by genotoxic drugs will not only enable the identification of novel functions of both chemicals and genes, but also have profound implications in cautionary measures of anticancer intervention in humans.<\/jats:p>","DOI":"10.1534\/g3.113.009100","type":"journal-article","created":{"date-parts":[[2013,11,7]],"date-time":"2013-11-07T19:57:04Z","timestamp":1383854224000},"page":"39-48","source":"Crossref","is-referenced-by-count":7,"title":["Chemical Suppression of Defects in Mitotic Spindle Assembly, Redox Control, and Sterol Biosynthesis by Hydroxyurea"],"prefix":"10.1093","volume":"4","author":[{"given":"Andrew","family":"McCulley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Haarer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Viggiano","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Karchin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyi","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,1,1]]},"reference":[{"key":"2021042011322121400_bib1","doi-asserted-by":"crossref","first-page":"5410","DOI":"10.1091\/mbc.e05-07-0678","article-title":"Role of essential genes in mitochondrial morphogenesis in Saccharomyces cerevisiae.","volume":"16","author":"Altmann","year":"2005","journal-title":"Mol. Biol. Cell"},{"key":"2021042011322121400_bib2","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.1038\/ncomms3410","article-title":"Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.","volume":"4","author":"Baraban","year":"2013","journal-title":"Nat Commun"},{"key":"2021042011322121400_bib3","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1073\/pnas.93.1.186","article-title":"Cloning and characterization of ERG25, the Saccharomyces cerevisiae gene encoding C-4 sterol methyl oxidase.","volume":"93","author":"Bard","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042011322121400_bib4","doi-asserted-by":"crossref","first-page":"12702","DOI":"10.1074\/jbc.M100274200","article-title":"The effect of the erg26\u20131 mutation on the regulation of lipid metabolism in Saccharomyces cerevisiae.","volume":"276","author":"Baudry","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2021042011322121400_bib5","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.molcel.2008.02.021","article-title":"Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae.","volume":"30","author":"Ben-Aroya","year":"2008","journal-title":"Mol. Cell"},{"key":"2021042011322121400_bib6","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1083\/jcb.200702101","article-title":"A novel role in cytokinesis reveals a housekeeping function for the unfolded protein response.","volume":"177","author":"Bicknell","year":"2007","journal-title":"J. Cell Biol."},{"key":"2021042011322121400_bib7","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1101\/gad.13.5.532","article-title":"The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast.","volume":"13","author":"Biggins","year":"1999","journal-title":"Genes Dev."},{"key":"2021042011322121400_bib8","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.molcel.2012.10.017","article-title":"Mitosis-specific regulation of nuclear transport by the spindle assembly checkpoint protein Mad1p.","volume":"49","author":"Cairo","year":"2013","journal-title":"Mol. Cell"},{"key":"2021042011322121400_bib9","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ymgme.2004.08.007","article-title":"Placental defects are associated with male lethality in bare patches and striated embryos deficient in the NAD(P)H steroid dehydrogenase-like (NSDHL) enzyme.","volume":"84","author":"Caldas","year":"2005","journal-title":"Mol. Genet. Metab."},{"key":"2021042011322121400_bib10","doi-asserted-by":"crossref","first-page":"21819","DOI":"10.1073\/pnas.0911987106","article-title":"Chemical modifier screen identifies HDAC inhibitors as suppressors of PKD models.","volume":"106","author":"Cao","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042011322121400_bib11","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1093\/genetics\/135.3.677","article-title":"Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast.","volume":"135","author":"Chan","year":"1993","journal-title":"Genetics"},{"key":"2021042011322121400_bib12","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1007\/s00438-005-0077-5","article-title":"Role of the iron mobilization and oxidative stress regulons in the genomic response of yeast to hydroxyurea.","volume":"275","author":"Dubacq","year":"2006","journal-title":"Mol. Genet. Genomics"},{"key":"2021042011322121400_bib13","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S1097-2765(00)80017-9","article-title":"The ERO1 gene of yeast is required for oxidation of protein dithiols in the endoplasmic reticulum.","volume":"1","author":"Frand","year":"1998","journal-title":"Mol. Cell"},{"key":"2021042011322121400_bib14","doi-asserted-by":"crossref","first-page":"11173","DOI":"10.1073\/pnas.94.21.11173","article-title":"A yeast sterol auxotroph (erg25) is rescued by addition of azole antifungals and reduced levels of heme.","volume":"94","author":"Gachotte","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042011322121400_bib15","doi-asserted-by":"crossref","first-page":"13794","DOI":"10.1073\/pnas.95.23.13794","article-title":"Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis.","volume":"95","author":"Gachotte","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042011322121400_bib16","doi-asserted-by":"crossref","first-page":"12655","DOI":"10.1073\/pnas.96.22.12655","article-title":"Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation.","volume":"96","author":"Gachotte","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042011322121400_bib17","doi-asserted-by":"crossref","first-page":"35904","DOI":"10.1074\/jbc.M504978200","article-title":"Characterizing sterol defect suppressors uncovers a novel transcriptional signaling pathway regulating zymosterol biosynthesis.","volume":"280","author":"Germann","year":"2005","journal-title":"J. Biol. Chem."},{"key":"2021042011322121400_bib18","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1073\/pnas.0307490100","article-title":"Chemogenomic profiling: identifying the functional interactions of small molecules in yeast.","volume":"101","author":"Giaever","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042011322121400_bib19","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.bbrc.2008.01.156","article-title":"Fission yeast dam1\u2013A8 mutant is resistant to and rescued by an anti-microtubule agent.","volume":"368","author":"Griffiths","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021042011322121400_bib20","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/S0092-8674(04)00418-0","article-title":"Structure of Ero1p, source of disulfide bonds for oxidative protein folding in the cell.","volume":"117","author":"Gross","year":"2004","journal-title":"Cell"},{"key":"2021042011322121400_bib21","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1073\/pnas.0506448103","article-title":"Generating disulfides enzymatically: reaction products and electron acceptors of the endoplasmic reticulum thiol oxidase Ero1p.","volume":"103","author":"Gross","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042011322121400_bib22","doi-asserted-by":"crossref","first-page":"e12732","DOI":"10.1371\/journal.pone.0012732","article-title":"The unfolded protein response is not necessary for the G1\/S transition, but it is required for chromosome maintenance in Saccharomyces cerevisiae.","volume":"5","author":"Henry","year":"2010","journal-title":"PLoS ONE"},{"key":"2021042011322121400_bib23","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1126\/science.1150021","article-title":"The chemical genomic portrait of yeast: uncovering a phenotype for all genes.","volume":"320","author":"Hillenmeyer","year":"2008","journal-title":"Science"},{"key":"2021042011322121400_bib24","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0092-8674(00)00015-5","article-title":"Functional discovery via a compendium of expression profiles.","volume":"102","author":"Hughes","year":"2000","journal-title":"Cell"},{"key":"2021042011322121400_bib25","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1002\/(SICI)1096-8628(20000214)90:4<339::AID-AJMG15>3.0.CO;2-5","article-title":"Mutations in the NSDHL gene, encoding a 3beta-hydroxysteroid dehydrogenase, cause CHILD syndrome.","volume":"90","author":"Konig","year":"2000","journal-title":"Am. J. Med. Genet."},{"key":"2021042011322121400_bib26","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.tcb.2010.10.007","article-title":"Sensing centromere tension: Aurora B and the regulation of kinetochore function.","volume":"21","author":"Lampson","year":"2011","journal-title":"Trends Cell Biol."},{"key":"2021042011322121400_bib27","doi-asserted-by":"crossref","first-page":"16927","DOI":"10.1074\/jbc.271.28.16927","article-title":"Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue.","volume":"271","author":"Li","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2021042011322121400_bib28","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/nbt.1832","article-title":"Systematic exploration of essential yeast gene function with temperature-sensitive mutants.","volume":"29","author":"Li","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"2021042011322121400_bib29","first-page":"237","article-title":"Chemical-genetic approaches for exploring the mode of action of natural products.","volume":"66","author":"Lopez","year":"2008","journal-title":"Prog. Drug Res."},{"key":"2021042011322121400_bib30","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S1096-7192(03)00137-9","article-title":"Identification of two novel mutations in the murine Nsdhl sterol dehydrogenase gene and development of a functional complementation assay in yeast.","volume":"80","author":"Lucas","year":"2003","journal-title":"Mol. Genet. Metab."},{"key":"2021042011322121400_bib31","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1083\/jcb.134.2.443","article-title":"Analysis of Tub4p, a yeast gamma-tubulin-like protein: implications for microtubule-organizing center function.","volume":"134","author":"Marschall","year":"1996","journal-title":"J. Cell Biol."},{"key":"2021042011322121400_bib32","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/j.ajhg.2010.11.004","article-title":"Hypomorphic temperature-sensitive alleles of NSDHL cause CK syndrome.","volume":"87","author":"McLarren","year":"2010","journal-title":"Am. J. Hum. Genet."},{"key":"2021042011322121400_bib33","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1007\/BF02535375","article-title":"Effect of altered sterol composition on the osmotic behavior of sphaeroplasts and mitochondria of Saccharomyces cerevisiae.","volume":"17","author":"McLean-Bowen","year":"1982","journal-title":"Lipids"},{"key":"2021042011322121400_bib34","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1128\/AAC.01526-09","article-title":"Preclinical evaluation of the antifolate QN254, 5-chloro- N\u20196\u2019-(2,5-dimethoxy-benzyl)-quinazoline-2,4,6-triamine, as an antimalarial drug candidate.","volume":"54","author":"Nzila","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"2021042011322121400_bib35","doi-asserted-by":"crossref","first-page":"e7640","DOI":"10.1371\/journal.pone.0007640","article-title":"Roles for the conserved spc105p\/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint.","volume":"4","author":"Pagliuca","year":"2009","journal-title":"PLoS ONE"},{"key":"2021042011322121400_bib36","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.cell.2006.06.040","article-title":"Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast.","volume":"126","author":"Parsons","year":"2006","journal-title":"Cell"},{"key":"2021042011322121400_bib37","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1161\/CIRCULATIONAHA.110.003731","article-title":"Novel chemical suppressors of long QT syndrome identified by an in vivo functional screen.","volume":"123","author":"Peal","year":"2011","journal-title":"Circulation"},{"key":"2021042011322121400_bib38","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1038\/nbt963","article-title":"Chemical suppression of a genetic mutation in a zebrafish model of aortic coarctation.","volume":"22","author":"Peterson","year":"2004","journal-title":"Nat. Biotechnol."},{"key":"2021042011322121400_bib39","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1016\/j.cub.2009.06.043","article-title":"Protein phosphatase 1 regulates exit from the spindle checkpoint in budding yeast.","volume":"19","author":"Pinsky","year":"2009","journal-title":"Curr. Biol."},{"key":"2021042011322121400_bib40","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S1097-2765(00)80018-0","article-title":"Ero1p: a novel and ubiquitous protein with an essential role in oxidative protein folding in the endoplasmic reticulum.","volume":"1","author":"Pollard","year":"1998","journal-title":"Mol. Cell"},{"key":"2021042011322121400_bib41","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1016\/j.cub.2011.04.011","article-title":"KNL1\/Spc105 recruits PP1 to silence the spindle assembly checkpoint.","volume":"21","author":"Rosenberg","year":"2011","journal-title":"Curr. Biol."},{"key":"2021042011322121400_bib42","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1083\/jcb.201003138","article-title":"Regulation of basal cellular physiology by the homeostatic unfolded protein response.","volume":"189","author":"Rutkowski","year":"2010","journal-title":"J. Cell Biol."},{"key":"2021042011322121400_bib43","doi-asserted-by":"crossref","first-page":"14823","DOI":"10.1074\/jbc.M109.091710","article-title":"Metabolic response to iron deficiency in Saccharomyces cerevisiae.","volume":"285","author":"Shakoury-Elizeh","year":"2010","journal-title":"J. Biol. Chem."},{"key":"2021042011322121400_bib44","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1242\/dmm.004267","article-title":"Compounds from an unbiased chemical screen reverse both ER-to-Golgi trafficking defects and mitochondrial dysfunction in Parkinson\u2019s disease models.","volume":"3","author":"Su","year":"2010","journal-title":"Dis. Model. Mech."},{"key":"2021042011322121400_bib45","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1091\/mbc.e08-07-0697","article-title":"Cu, Zn superoxide dismutase and NADP(H) homeostasis are required for tolerance of endoplasmic reticulum stress in Saccharomyces cerevisiae.","volume":"20","author":"Tan","year":"2009","journal-title":"Mol. Biol. Cell"},{"key":"2021042011322121400_bib46","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.1073\/pnas.1014406108","article-title":"Temperature-sensitive ipl1\u20132\/Aurora B mutation is suppressed by mutations in TOR complex 1 via the Glc7\/PP1 phosphatase.","volume":"108","author":"Tatchell","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042011322121400_bib47","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1038\/ncb2549","article-title":"Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress.","volume":"14","author":"Tkach","year":"2012","journal-title":"Nat. Cell Biol."},{"key":"2021042011322121400_bib48","first-page":"171","article-title":"Synthetic genetic array analysis in Saccharomyces cerevisiae.","volume":"313","author":"Tong","year":"2006","journal-title":"Methods Mol. Biol."},{"key":"2021042011322121400_bib49","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/S1097-2765(01)00216-7","article-title":"An origin-deficient yeast artificial chromosome triggers a cell cycle checkpoint.","volume":"7","author":"van Brabant","year":"2001","journal-title":"Mol. Cell"},{"key":"2021042011322121400_bib50","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1534\/g3.112.004754","article-title":"Saccharomyces cerevisiae genetics predicts candidate therapeutic genetic interactions at the mammalian replication fork.","volume":"3","author":"van Pel","year":"2013","journal-title":"G3 (Bethesda)"},{"key":"2021042011322121400_bib51","doi-asserted-by":"crossref","first-page":"e120","DOI":"10.1093\/nar\/gng121","article-title":"Reconstitution of an efficient thymidine salvage pathway in Saccharomyces cerevisiae.","volume":"31","author":"Vernis","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"2021042011322121400_bib52","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1002\/yea.1406","article-title":"New vectors for simplified construction of BrdU-Incorporating strains of Saccharomyces cerevisiae.","volume":"23","author":"Viggiani","year":"2006","journal-title":"Yeast"},{"key":"2021042011322121400_bib53","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/S1534-5807(01)00073-9","article-title":"Phosphorylation of gamma-tubulin regulates microtubule organization in budding yeast.","volume":"1","author":"Vogel","year":"2001","journal-title":"Dev. Cell"},{"key":"2021042011322121400_bib54","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1126\/science.285.5429.901","article-title":"Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.","volume":"285","author":"Winzeler","year":"1999","journal-title":"Science"},{"key":"2021042011322121400_bib55","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1002\/j.1460-2075.1995.tb07106.x","article-title":"AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae.","volume":"14","author":"Yamaguchi-Iwai","year":"1995","journal-title":"EMBO J."},{"key":"2021042011322121400_bib56","doi-asserted-by":"crossref","first-page":"3377","DOI":"10.1002\/j.1460-2075.1996.tb00703.x","article-title":"Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast.","volume":"15","author":"Yamaguchi-Iwai","year":"1996","journal-title":"EMBO J."},{"key":"2021042011322121400_bib57","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1073\/pnas.0406361102","article-title":"Microtubule-binding drugs offset tau sequestration by stabilizing microtubules and reversing fast axonal transport deficits in a tauopathy model.","volume":"102","author":"Zhang","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["G3 Genes|Genomes|Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/g3journal\/article-pdf\/4\/1\/39\/37154270\/g3journal0039.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/g3journal\/article-pdf\/4\/1\/39\/37154270\/g3journal0039.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T14:35:20Z","timestamp":1618929320000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/g3journal\/article\/4\/1\/39\/6025870"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,1]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1,1]]}},"URL":"https:\/\/doi.org\/10.1534\/g3.113.009100","relation":{},"ISSN":["2160-1836"],"issn-type":[{"value":"2160-1836","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2014,1,1]]},"published":{"date-parts":[[2014,1,1]]}}}