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However, mild hypoxia can be protective against later, more severe hypoxia exposure via \u201chypoxic preconditioning,\u201d a phenomenon that is not yet fully understood. Accordingly, we have established and optimized an embryonic zebrafish model to study hypoxic preconditioning. Using a functional genomic approach, we used this zebrafish model to identify and validate five novel hypoxia-protective genes, including irs2, crtc3, and camk2g2, which have been previously implicated in metabolic regulation. These results extend our understanding of the mechanisms of hypoxic preconditioning and affirm the discovery potential of this novel vertebrate hypoxic stress model.<\/jats:p>","DOI":"10.1534\/g3.115.018010","type":"journal-article","created":{"date-parts":[[2015,4,6]],"date-time":"2015-04-06T22:37:47Z","timestamp":1428359867000},"page":"1107-1116","source":"Crossref","is-referenced-by-count":29,"title":["Novel Genes Critical for Hypoxic Preconditioning in Zebrafish Are Regulators of Insulin and Glucose Metabolism"],"prefix":"10.1093","volume":"5","author":[{"given":"Tania","family":"Manchenkov","sequence":"first","affiliation":[{"name":"Division of Neonatology, University of California San Diego School of Medicine, La Jolla, California 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina P","family":"Pasillas","sequence":"additional","affiliation":[{"name":"Division of Neonatology, University of California San Diego School of Medicine, La Jolla, California 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel G","family":"Haddad","sequence":"additional","affiliation":[{"name":"Division of Respiratory Medicine, University of California San Diego School of Medicine, La Jolla, California 92093"},{"name":"Department of Pediatrics, University of California San Diego School of Medicine, La Jolla, California 92093"},{"name":"Rady Children\u2019s Hospital-San Diego, San Diego, California 92123"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhad B","family":"Imam","sequence":"additional","affiliation":[{"name":"Division of Neonatology, University of California San Diego School of Medicine, La Jolla, California 92093"},{"name":"Division of Neonatology, University of California San Diego School of Medicine, La Jolla, California 92093"},{"name":"Department of Pediatrics, University of California San Diego School of Medicine, La Jolla, California 92093"},{"name":"Rady Children\u2019s Hospital-San Diego, San Diego, California 92123"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,6,1]]},"reference":[{"key":"2021042013553816100_bib1"},{"key":"2021042013553816100_bib2","author":"Azad","year":"2012"},{"key":"2021042013553816100_bib3","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.cell.2010.05.028","article-title":"TIF1gamma controls erythroid cell fate by regulating transcription elongation.","volume":"142","author":"Bai","year":"2010","journal-title":"Cell"},{"key":"2021042013553816100_bib4","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1089\/zeb.2008.0555","article-title":"A primer for morpholino use in zebrafish.","volume":"6","author":"Bill","year":"2009","journal-title":"Zebrafish"},{"key":"2021042013553816100_bib5","doi-asserted-by":"crossref","first-page":"e22022","DOI":"10.1371\/journal.pone.0022022","article-title":"Origin and evolution of TRIM proteins: new insights from the complete TRIM repertoire of zebrafish and pufferfish.","volume":"6","author":"Boudinot","year":"2011","journal-title":"PLoS ONE"},{"key":"2021042013553816100_bib6","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/S0140-6736(07)61305-1","article-title":"The cardiovascular toll of stress.","volume":"370","author":"Brotman","year":"2007","journal-title":"Lancet"},{"key":"2021042013553816100_bib7","doi-asserted-by":"crossref","first-page":"7321","DOI":"10.1073\/pnas.0802164105","article-title":"Hypoxia and the HIF-1 transcriptional pathway reorganize a neuronal circuit for oxygen-dependent behavior in Caenorhabditis elegans.","volume":"105","author":"Chang","year":"2008","journal-title":"Proc. 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