{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T21:10:02Z","timestamp":1721337002570},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In Caenorhabditis elegans, germline expression programs are actively repressed in somatic tissue by components of the synMuv (synthetic multi-vulva) B chromatin remodeling complex, which include homologs of tumor suppressors Retinoblastoma (Rb\/LIN-35) and Malignant Brain Tumor (MBT\/LIN-61). However, the full scope of pathways that suppress germline expression in the soma is unknown. To address this, we performed a mutagenesis and screened for somatic expression of GFP-tagged PGL-1, a core P-granule nucleating protein. Eight alleles were isolated from 4000 haploid genomes. Five of these alleles exhibit a synMuv phenotype, whereas the remaining three were identified as hypomorphic alleles of known synMuv B genes, lin-13 and dpl-1. These findings suggest that most suppressors of germline programs in the soma of C. elegans are either required for viability or function through synMuv B chromatin regulation.<\/jats:p>","DOI":"10.1534\/g3.115.019257","type":"journal-article","created":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T00:57:17Z","timestamp":1435798637000},"page":"2209-2215","source":"Crossref","is-referenced-by-count":2,"title":["A Forward Genetic Screen for Suppressors of Somatic P Granules in<i>Caenorhabditis elegans<\/i>"],"prefix":"10.1093","volume":"5","author":[{"given":"Ashley L","family":"Kelly","sequence":"first","affiliation":[{"name":"Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J","family":"Senter-Zapata","sequence":"additional","affiliation":[{"name":"Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne C","family":"Campbell","sequence":"additional","affiliation":[{"name":"Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah E","family":"Lust","sequence":"additional","affiliation":[{"name":"Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique E","family":"Theriault","sequence":"additional","affiliation":[{"name":"Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karolina M","family":"Andralojc","sequence":"additional","affiliation":[{"name":"Kathryn W. Davis Center for Regenerative Biology and Medicine, Mount Desert Island Biological Laboratory, Bar Harbor, Maine 04672"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dustin L","family":"Updike","sequence":"additional","affiliation":[{"name":"Kathryn W. 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