{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T10:19:40Z","timestamp":1768731580316,"version":"3.49.0"},"reference-count":108,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Eukaryotic DNA replication begins from multiple origins. The origin recognition complex (ORC) binds origin DNA and scaffolds assembly of a prereplicative complex (pre-RC), which is subsequently activated to initiate DNA replication. In multicellular eukaryotes, origins do not share a strict DNA consensus sequence, and their activity changes in concert with chromatin status during development, but mechanisms are ill-defined. Previous genome-wide analyses in Drosophila and other organisms have revealed a correlation between ORC binding sites and the histone variant H3.3. This correlation suggests that H3.3 may designate origin sites, but this idea has remained untested. To address this question, we examined the enrichment and function of H3.3 at the origins responsible for developmental gene amplification in the somatic follicle cells of the Drosophila ovary. We found that H3.3 is abundant at these amplicon origins. H3.3 levels remained high when replication initiation was blocked, indicating that H3.3 is abundant at the origins before activation of the pre-RC. H3.3 was also enriched at the origins during early oogenesis, raising the possibility that H3.3 bookmarks sites for later amplification. However, flies null mutant for both of the H3.3 genes in Drosophila did not have overt defects in developmental gene amplification or genomic replication, suggesting that H3.3 is not essential for the assembly or activation of the pre-RC at origins. Instead, our results imply that the correlation between H3.3 and ORC sites reflects other chromatin attributes that are important for origin function.<\/jats:p>","DOI":"10.1534\/g3.116.028068","type":"journal-article","created":{"date-parts":[[2016,6,17]],"date-time":"2016-06-17T22:16:10Z","timestamp":1466201770000},"page":"1661-1671","source":"Crossref","is-referenced-by-count":8,"title":["The Histone Variant H3.3 Is Enriched at<i>Drosophila<\/i>Amplicon Origins but Does Not Mark Them for Activation"],"prefix":"10.1093","volume":"6","author":[{"given":"Neha P","family":"Paranjape","sequence":"first","affiliation":[{"name":"Department of Biology, Indiana University, Bloomington, Indiana 47405"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian R","family":"Calvi","sequence":"additional","affiliation":[{"name":"Department of Biology, Indiana University, Bloomington, Indiana 47405"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,6,1]]},"reference":[{"key":"2021042011573489300_bib1","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1038\/nature02694","article-title":"Chromatin regulates origin activity in Drosophila follicle cells.","volume":"430","author":"Aggarwal","year":"2004","journal-title":"Nature"},{"key":"2021042011573489300_bib2","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/S1097-2765(02)00542-7","article-title":"The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly.","volume":"9","author":"Ahmad","year":"2002","journal-title":"Mol. 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Biol."},{"key":"2021042011573489300_bib48","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4161\/epi.4.2.7772","article-title":"Replication timing as an epigenetic mark.","volume":"4","author":"Hiratani","year":"2009","journal-title":"Epigenetics"},{"key":"2021042011573489300_bib49","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1101\/gr.099796.109","article-title":"Genome-wide dynamics of replication timing revealed by in vitro models of mouse embryogenesis.","volume":"20","author":"Hiratani","year":"2010","journal-title":"Genome Res."},{"key":"2021042011573489300_bib50","doi-asserted-by":"crossref","first-page":"e1003798","DOI":"10.1371\/journal.pgen.1003798","article-title":"A link between ORC-origin binding mechanisms and origin activation time revealed in budding yeast.","volume":"9","author":"Hoggard","year":"2013","journal-title":"PLoS Genet."},{"key":"2021042011573489300_bib51","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1002\/dvdy.20286","article-title":"Mass transit: epithelial morphogenesis in the Drosophila egg chamber.","volume":"232","author":"Horne-Badovinac","year":"2005","journal-title":"Dev. Dyn."},{"key":"2021042011573489300_bib52","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1101\/SQB.1968.033.01.059","article-title":"Visualization of replicating mammalian and T4 bacteriophage DNA.","volume":"33","author":"Huberman","year":"1968","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"2021042011573489300_bib53","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1083\/jcb.201407004","article-title":"Peaks cloaked in the mist: the landscape of mammalian replication origins.","volume":"208","author":"Hyrien","year":"2015","journal-title":"J. Cell Biol."},{"key":"2021042011573489300_bib54","doi-asserted-by":"crossref","first-page":"23027","DOI":"10.1074\/jbc.274.33.23027","article-title":"Histone acetyltransferase HBO1 interacts with the ORC1 subunit of the human initiator protein.","volume":"274","author":"Iizuka","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2021042011573489300_bib55","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1101\/gad.264150.115","article-title":"Histone H3.3 maintains genome integrity during mammalian development.","volume":"29","author":"Jang","year":"2015","journal-title":"Genes Dev."},{"key":"2021042011573489300_bib56","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1101\/gad.1547707","article-title":"Nucleosome stability mediated by histone variants H3.3 and H2A.","volume":"21","author":"Jin","year":"2007","journal-title":"Z. Genes Dev"},{"key":"2021042011573489300_bib57","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1038\/ng.409","article-title":"H3.3\/H2A.Z double variant-containing nucleosomes mark \u2018nucleosome-free regions\u2019 of active promoters and other regulatory regions.","volume":"41","author":"Jin","year":"2009","journal-title":"Nat. Genet."},{"key":"2021042011573489300_bib58","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.molcel.2011.02.006","article-title":"Stalled fork rescue via dormant replication origins in unchallenged S phase promotes proper chromosome segregation and tumor suppression.","volume":"41","author":"Kawabata","year":"2011","journal-title":"Mol. 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Biol."},{"key":"2021042011573489300_bib68","doi-asserted-by":"crossref","first-page":"3897","DOI":"10.1093\/nar\/28.20.3897","article-title":"Localisation of the DmCdc45 DNA replication factor in the mitotic cycle and during chorion gene amplification.","volume":"28","author":"Loebel","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"2021042011573489300_bib69","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1101\/gad.822101","article-title":"Functionally distinct, sequence-specific replicator and origin elements are required for Drosophila chorion gene amplification.","volume":"15","author":"Lu","year":"2001","journal-title":"Genes Dev."},{"key":"2021042011573489300_bib70","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1101\/gr.097873.109","article-title":"Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading.","volume":"20","author":"MacAlpine","year":"2010","journal-title":"Genome Res."},{"key":"2021042011573489300_bib71","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ydbio.2004.07.017","article-title":"Eggs over easy: cell death in the Drosophila ovary.","volume":"274","author":"McCall","year":"2004","journal-title":"Dev. 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Biol."},{"key":"2021042011573489300_bib88","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1073\/pnas.0712168105","article-title":"A chemical method for fast and sensitive detection of DNA synthesis in vivo.","volume":"105","author":"Salic","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042011573489300_bib89","doi-asserted-by":"crossref","first-page":"19721","DOI":"10.1073\/pnas.1206629109","article-title":"Nucleosome-depleted chromatin gaps recruit assembly factors for the H3.3 histone variant.","volume":"109","author":"Schneiderman","year":"2012","journal-title":"Proc. Natl. Acad. Sci. 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