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We conducted a chemical mutagenesis and screen to recover new mutations that affect cell competition between wild-type and RpS3 heterozygous cells. Mutations were identified by whole-genome sequencing, making use of software tools that greatly facilitate the distinction between newly induced mutations and other sources of apparent sequence polymorphism, thereby reducing false-positive and false-negative identification rates. In addition, we utilized iPLEX MassARRAY for genotyping recombinant chromosomes. These approaches permitted the mapping of a new mutation affecting cell competition when only a single allele existed, with a phenotype assessed only in genetic mosaics, without the benefit of complementation with existing mutations, deletions, or duplications. These techniques expand the utility of chemical mutagenesis and whole-genome sequencing for mutant identification. We discuss mutations in the Atm and Xrp1 genes identified in this screen.<\/jats:p>","DOI":"10.1534\/g3.116.029421","type":"journal-article","created":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T21:14:26Z","timestamp":1475874866000},"page":"3207-3217","source":"Crossref","is-referenced-by-count":35,"title":["Whole-Genome Sequencing and iPLEX MassARRAY Genotyping Map an EMS-Induced Mutation Affecting Cell Competition in <i>Drosophila melanogaster<\/i>"],"prefix":"10.1093","volume":"6","author":[{"given":"Chang-Hyun","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Rimesso","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlu","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas E","family":"Baker","sequence":"additional","affiliation":[{"name":"Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,10,1]]},"reference":[{"key":"2021062505523567600_bib1","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1101\/gad.914401","article-title":"Cell cycle checkpoint signaling through the ATM and ATR kinases.","volume":"15","author":"Abraham","year":"2001","journal-title":"Genes Dev."},{"key":"2021062505523567600_bib2","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1126\/science.287.5461.2185","article-title":"The genome sequence of Drosophila melanogaster.","volume":"287","author":"Adams","year":"2000","journal-title":"Science"},{"key":"2021062505523567600_bib3","doi-asserted-by":"crossref","first-page":"5184","DOI":"10.1038\/sj.onc.1210328","article-title":"p53 directs focused genomic responses in Drosophila.","volume":"26","author":"Akdemir","year":"2007","journal-title":"Oncogene"},{"key":"2021062505523567600_bib4","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1242\/dev.079129","article-title":"Cell competition: how to eliminate your neighbours.","volume":"141","author":"Amoyel","year":"2014","journal-title":"Development"},{"key":"2021062505523567600_bib5","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1093\/genetics\/137.2.513","article-title":"A Drosophila third chromosome Minute locus encodes a ribosomal protein.","volume":"137","author":"Andersson","year":"1994","journal-title":"Genetics"},{"key":"2021062505523567600_bib6","doi-asserted-by":"crossref","first-page":"R11","DOI":"10.1016\/j.cub.2010.11.030","article-title":"Cell competition.","volume":"21","author":"Baker","year":"2011","journal-title":"Curr. 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