{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:47:02Z","timestamp":1792608422855,"version":"4.5.0"},"reference-count":96,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The Cell Wall Integrity (CWI) pathway is the primary signaling cascade that controls the de novo synthesis of the fungal cell wall, and in Saccharomyces cerevisiae this event is highly dependent on the RLM1 transcription factor. Here, we investigated the function of RlmA in the fungal pathogen Aspergillus fumigatus. We show that the \u0394rlmA strain exhibits an altered cell wall organization in addition to defects related to vegetative growth and tolerance to cell wall-perturbing agents. A genetic analysis indicated that rlmA is positioned downstream of the pkcA and mpkA genes in the CWI pathway. As a consequence, rlmA loss-of-function leads to the altered expression of genes encoding cell wall-related proteins. RlmA positively regulates the phosphorylation of MpkA and is induced at both protein and transcriptional levels during cell wall stress. The rlmA was also involved in tolerance to oxidative damage and transcriptional regulation of genes related to oxidative stress adaptation. Moreover, the \u0394rlmA strain had attenuated virulence in a neutropenic murine model of invasive pulmonary aspergillosis. Our results suggest that RlmA functions as a transcription factor in the A. fumigatus CWI pathway, acting downstream of PkcA-MpkA signaling and contributing to the virulence of this fungus.<\/jats:p>","DOI":"10.1534\/g3.116.031112","type":"journal-article","created":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T18:39:08Z","timestamp":1470076748000},"page":"2983-3002","source":"Crossref","is-referenced-by-count":97,"title":["<i>Aspergillus fumigatus<\/i>\n                    MADS-Box Transcription Factor\n                    <i>rlmA<\/i>\n                    Is Required for Regulation of the Cell Wall Integrity and Virulence"],"prefix":"10.1093","volume":"6","author":[{"given":"Marina Campos","family":"Rocha","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal de S\u00e3o Carlos, 13565 S\u00e3o Carlos, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Henrique Tadini Marilhano","family":"Fabri","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal de S\u00e3o Carlos, 13565 S\u00e3o Carlos, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krissia","family":"Franco de Godoy","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal de S\u00e3o Carlos, 13565 S\u00e3o Carlos, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia","family":"Alves de Castro","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Issa","family":"Hori","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, 14049 Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson","family":"Ferreira da Cunha","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal de S\u00e3o Carlos, 13565 S\u00e3o Carlos, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Arentshorst","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Biotechnology, Institute of Biology, Leiden University, 2333 Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur F J","family":"Ram","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Biotechnology, Institute of Biology, Leiden University, 2333 Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cees A M J J","family":"van den Hondel","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Biotechnology, Institute of Biology, Leiden University, 2333 Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Henrique","family":"Goldman","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iran","family":"Malavazi","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal de S\u00e3o Carlos, 13565 S\u00e3o Carlos, S\u00e3o Paulo, Brazil"},{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal de S\u00e3o Carlos, 13565 S\u00e3o Carlos, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,9,1]]},"reference":[{"key":"2021042015111475300_bib1","doi-asserted-by":"crossref","first-page":"e0136932","DOI":"10.1371\/journal.pone.0136932","article-title":"Network Modeling Reveals Cross Talk of MAP Kinases during Adaptation to Caspofungin Stress in Aspergillus fumigatus.","volume":"10","author":"Altwasser","year":"2015","journal-title":"PLoS One"},{"key":"2021042015111475300_bib2","doi-asserted-by":"crossref","first-page":"4335","DOI":"10.1128\/JCM.42.9.4335-4337.2004","article-title":"Variability of germinative potential among pathogenic species of Aspergillus.","volume":"42","author":"Araujo","year":"2004","journal-title":"J. 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