{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T12:43:25Z","timestamp":1760013805387},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Parasitic worms of the genus Trichinella (phylum Nematoda; class Enoplea) represent a complex of at least twelve taxa that infect a range of different host animals, including humans, around the world. They are foodborne, intracellular nematodes, and their life cycles differ substantially from those of other nematodes. The recent characterization of the genomes and transcriptomes of all twelve recognized taxa of Trichinella now allows, for the first time, detailed studies of their molecular biology. In the present study, we defined, curated, and compared the protein kinase complements (kinomes) of Trichinella spiralis and T. pseudospiralis using an integrated bioinformatic workflow employing transcriptomic and genomic data sets. We examined how variation in the kinome might link to unique aspects of Trichinella morphology, biology, and evolution. Furthermore, we utilized in silico structural modeling to discover and characterize a novel, MOS-like kinase with an unusual, previously undescribed N-terminal domain. Taken together, the present findings provide a basis for comparative investigations of nematode kinomes, and might facilitate the identification of Enoplea-specific intervention and diagnostic targets. Importantly, the in silico modeling approach assessed here provides an exciting prospect of being able to identify and classify currently unknown (orphan) kinases, as a foundation for their subsequent structural and functional investigation.<\/jats:p>","DOI":"10.1534\/g3.116.032961","type":"journal-article","created":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T22:39:08Z","timestamp":1470091148000},"page":"2847-2856","source":"Crossref","is-referenced-by-count":6,"title":["Analyses of Compact <i>Trichinella<\/i> Kinomes Reveal a MOS-Like Protein Kinase with a Unique N-Terminal Domain"],"prefix":"10.1093","volume":"6","author":[{"given":"Andreas J","family":"Stroehlein","sequence":"first","affiliation":[{"name":"Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil D","family":"Young","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pasi K","family":"Korhonen","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bill C H","family":"Chang","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia"},{"name":"Yourgene Bioscience, Shu-Lin District, New Taipei City 23863, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul W","family":"Sternberg","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Chevy Chase, MD 20815-6789"},{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California 91125"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"La Rosa","sequence":"additional","affiliation":[{"name":"Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edoardo","family":"Pozio","sequence":"additional","affiliation":[{"name":"Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin B","family":"Gasser","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia"},{"name":"Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2016,9,1]]},"reference":[{"key":"2021042015111570600_bib1","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1534\/genetics.110.119768","article-title":"Reversal of salt preference is directed by the insulin\/PI3K and Gq\/PKC signaling in Caenorhabditis elegans","volume":"186","author":"Adachi","year":"2010","journal-title":"Genetics"},{"key":"2021042015111570600_bib2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0166-6851(97)00145-X","article-title":"Identification of serine\/threonine protein kinases secreted by Trichinella spiralis infective larvae","volume":"90","author":"Arden","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"2021042015111570600_bib3","doi-asserted-by":"crossref","first-page":"S26","DOI":"10.1017\/S0031182014000791","article-title":"The evolution of parasitism in Nematoda","volume":"142","author":"Blaxter","year":"2015","journal-title":"Parasitology"},{"key":"2021042015111570600_bib4","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1038\/32160","article-title":"A molecular evolutionary framework for the phylum Nematoda","volume":"392","author":"Blaxter","year":"1998","journal-title":"Nature"},{"key":"2021042015111570600_bib5","doi-asserted-by":"crossref","first-page":"e1685","DOI":"10.1038\/cddis.2015.51","article-title":"Readthrough acetylcholinesterase (AChE-R) and regulated necrosis: pharmacological targets for the regulation of ovarian functions?","volume":"6","author":"Blohberger","year":"2015","journal-title":"Cell Death Dis."},{"key":"2021042015111570600_bib6","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1186\/1471-2105-10-274","article-title":"MultiLoc2: integrating phylogeny and Gene Ontology terms improves subcellular protein localization prediction","volume":"10","author":"Blum","year":"2009","journal-title":"BMC Bioinformatics"},{"key":"2021042015111570600_bib7","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1186\/1471-2105-10-421","article-title":"BLAST+: architecture and applications","volume":"10","author":"Camacho","year":"2009","journal-title":"BMC Bioinformatics"},{"key":"2021042015111570600_bib8","doi-asserted-by":"crossref","first-page":"209","DOI":"10.2307\/3284472","article-title":"Trichinella spiralis: vascular endothelial growth factor is up-regulated within the nurse cell during the early phase of its formation","volume":"84","author":"Capo","year":"1998","journal-title":"J. Parasitol."},{"key":"2021042015111570600_bib9","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/S0968-0004(00)01712-6","article-title":"The regulation of protein function by multisite phosphorylation - a 25 year update","volume":"25","author":"Cohen","year":"2000","journal-title":"Trends Biochem. Sci."},{"key":"2021042015111570600_bib10","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/ng.2585","article-title":"Genomics of Loa loa, a Wolbachia-free filarial parasite of humans","volume":"45","author":"Desjardins","year":"2013","journal-title":"Nat. Genet."},{"key":"2021042015111570600_bib11","doi-asserted-by":"crossref","first-page":"472","DOI":"10.2307\/3283370","article-title":"Trichinella spiralis and the concept of niche","volume":"79","author":"Despommier","year":"1993","journal-title":"J. Parasitol."},{"key":"2021042015111570600_bib12","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S0169-4758(98)01287-3","article-title":"How does Trichinella spiralis make itself at home?","volume":"14","author":"Despommier","year":"1998","journal-title":"Parasitol. Today"},{"key":"2021042015111570600_bib13","doi-asserted-by":"crossref","first-page":"350412","DOI":"10.1155\/2011\/350412","article-title":"Mos in the oocyte: how to use MAPK independently of growth factors and transcription to control meiotic divisions","volume":"2011","author":"Dupr\u00e9","year":"2011","journal-title":"J. Signal Transduct."},{"key":"2021042015111570600_bib14","doi-asserted-by":"crossref","first-page":"4423","DOI":"10.1242\/jcs.111195","article-title":"Cell cycle regulation by the NEK family of protein kinases","volume":"125","author":"Fry","year":"2012","journal-title":"J. Cell Sci."},{"key":"2021042015111570600_bib15","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1002\/bies.950190106","article-title":"Synthesis and function of Mos: the control switch of vertebrate oocyte meiosis","volume":"19","author":"Gebauer","year":"1997","journal-title":"BioEssays"},{"key":"2021042015111570600_bib16","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1093\/bioinformatics\/btt419","article-title":"Kinannote, a computer program to identify and classify members of the eukaryotic protein kinase superfamily","volume":"29","author":"Goldberg","year":"2013","journal-title":"Bioinformatics"},{"key":"2021042015111570600_bib17","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1006\/jmbi.2001.5080","article-title":"Assignment of homology to genome sequences using a library of hidden Markov models that represent all proteins of known structure","volume":"313","author":"Gough","year":"2001","journal-title":"J. Mol. Biol."},{"key":"2021042015111570600_bib18","doi-asserted-by":"crossref","first-page":"3658","DOI":"10.1128\/IAI.69.6.3658-3662.2001","article-title":"Secreted variant of nucleoside diphosphate kinase from the intracellular parasitic nematode Trichinella spiralis","volume":"69","author":"Gounaris","year":"2001","journal-title":"Infect. Immun."},{"key":"2021042015111570600_bib19","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1096\/fasebj.9.8.7768349","article-title":"Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification.","volume":"9","author":"Hanks","year":"1995","journal-title":"FASEB J."},{"key":"2021042015111570600_bib20","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1126\/science.3291115","article-title":"The protein kinase family: conserved features and deduced phylogeny of the catalytic domains","volume":"241","author":"Hanks","year":"1988","journal-title":"Science"},{"key":"2021042015111570600_bib21","doi-asserted-by":"crossref","first-page":"15072","DOI":"10.1073\/pnas.1408987111","article-title":"Activation of the pseudokinase MLKL unleashes the four-helix bundle domain to induce membrane localization and necroptotic cell death","volume":"111","author":"Hildebrand","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042015111570600_bib22","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/ng.3012","article-title":"Genome and transcriptome of the porcine whipworm Trichuris suis","volume":"46","author":"Jex","year":"2014","journal-title":"Nat. Genet."},{"key":"2021042015111570600_bib23","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","article-title":"InterProScan 5: genome-scale protein function classification","volume":"30","author":"Jones","year":"2014","journal-title":"Bioinformatics"},{"key":"2021042015111570600_bib24","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"2021042015111570600_bib25","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1101\/gr.229202","article-title":"BLAT - the BLAST-like alignment tool","volume":"12","author":"Kent","year":"2002","journal-title":"Genome Res."},{"key":"2021042015111570600_bib26","doi-asserted-by":"crossref","first-page":"10513","DOI":"10.1038\/ncomms10513","article-title":"Phylogenomic and biogeographic reconstruction of the Trichinella complex","volume":"7","author":"Korhonen","year":"2016","journal-title":"Nat. Commun."},{"key":"2021042015111570600_bib27","doi-asserted-by":"crossref","first-page":"2178","DOI":"10.1101\/gr.1224503","article-title":"OrthoMCL: identification of ortholog groups for eukaryotic genomes","volume":"13","author":"Li","year":"2003","journal-title":"Genome Res."},{"key":"2021042015111570600_bib28","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1146\/annurev-biochem-061611-090435","article-title":"The MPS1 family of protein kinases","volume":"81","author":"Liu","year":"2012","journal-title":"Annu. Rev. Biochem."},{"key":"2021042015111570600_bib29","doi-asserted-by":"crossref","unstructured":"Manning, G., 2005\u2003Genomic overview of protein kinases, WormBook, ed. The C. elegans Research Community, https:\/\/doi.org\/10.1895\/wormbook.1.60.1","DOI":"10.1895\/wormbook.1.60.1"},{"key":"2021042015111570600_bib30","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/S0968-0004(02)02179-5","article-title":"Evolution of protein kinase signaling from yeast to man","volume":"27","author":"Manning","year":"2002","journal-title":"Trends Biochem. Sci."},{"key":"2021042015111570600_bib31","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1126\/science.1075762","article-title":"The protein kinase complement of the human genome","volume":"298","author":"Manning","year":"2002","journal-title":"Science"},{"key":"2021042015111570600_bib32","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1038\/nprot.2013.092","article-title":"Large-scale gene function analysis with the PANTHER classification system","volume":"8","author":"Mi","year":"2013","journal-title":"Nat. Protoc."},{"key":"2021042015111570600_bib33","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/ng.769","article-title":"The draft genome of the parasitic nematode Trichinella spiralis","volume":"43","author":"Mitreva","year":"2011","journal-title":"Nat. Genet."},{"key":"2021042015111570600_bib34","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.immuni.2013.06.018","article-title":"The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism","volume":"39","author":"Murphy","year":"2013","journal-title":"Immunity"},{"key":"2021042015111570600_bib35","doi-asserted-by":"crossref","DOI":"10.1146\/annurev-biochem-060815-014830","article-title":"Necroptosis and inflammation","volume":"85","author":"Newton","year":"2016","journal-title":"Annu. Rev. Biochem."},{"key":"2021042015111570600_bib36","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1016\/j.cub.2009.05.043","article-title":"Lateralized gustatory behavior of C. elegans is controlled by specific receptor-type guanylyl cyclases","volume":"19","author":"Ortiz","year":"2009","journal-title":"Curr. Biol."},{"key":"2021042015111570600_bib37","doi-asserted-by":"crossref","first-page":"R769","DOI":"10.1016\/j.cub.2005.08.057","article-title":"Signal transduction: how Rad53 kinase is activated","volume":"15","author":"Pellicioli","year":"2005","journal-title":"Curr. Biol."},{"key":"2021042015111570600_bib38","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera - a visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J. Comput. Chem."},{"key":"2021042015111570600_bib39","doi-asserted-by":"crossref","first-page":"13603","DOI":"10.1073\/pnas.96.24.13603","article-title":"The protein kinases of Caenorhabditis elegans: a model for signal transduction in multicellular organisms","volume":"96","author":"Plowman","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042015111570600_bib40","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetpar.2007.07.002","article-title":"World distribution of Trichinella spp. infections in animals and humans","volume":"149","author":"Pozio","year":"2007","journal-title":"Vet. Parasitol."},{"key":"2021042015111570600_bib41","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.ijpara.2013.05.010","article-title":"New pieces of the Trichinella puzzle","volume":"43","author":"Pozio","year":"2013","journal-title":"Int. J. Parasitol."},{"key":"2021042015111570600_bib42","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0065-308X(06)63005-4","article-title":"Systematics and epidemiology of Trichinella","volume":"63","author":"Pozio","year":"2006","journal-title":"Adv. Parasitol."},{"key":"2021042015111570600_bib43","doi-asserted-by":"crossref","first-page":"S27","DOI":"10.1051\/parasite\/200108s2027","article-title":"The detection of encapsulated and non-encapsulated species of Trichinella suggests the existence of two evolutive lines in the genus","volume":"8","author":"Pozio","year":"2001","journal-title":"Parasite"},{"key":"2021042015111570600_bib44","doi-asserted-by":"crossref","first-page":"5161","DOI":"10.1242\/jcs.02681","article-title":"Caught Nek-ing: cilia and centrioles","volume":"118","author":"Quarmby","year":"2005","journal-title":"J. Cell Sci."},{"key":"2021042015111570600_bib45","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/S0168-9525(00)02024-2","article-title":"EMBOSS: the European Molecular Biology Open Software Suite","volume":"16","author":"Rice","year":"2000","journal-title":"Trends Genet."},{"key":"2021042015111570600_bib46","volume-title":"Gerald D. Schmidt & Larry S. Roberts\u2019 Foundations of Parasitology","author":"Roberts","year":"2009"},{"key":"2021042015111570600_bib47","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1186\/gb-2013-14-6-405","article-title":"The advantages of SMRT sequencing","volume":"14","author":"Roberts","year":"2013","journal-title":"Genome Biol."},{"key":"2021042015111570600_bib48","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1093\/sysbio\/sys029","article-title":"MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space","volume":"61","author":"Ronquist","year":"2012","journal-title":"Syst. Biol."},{"key":"2021042015111570600_bib49","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nprot.2010.5","article-title":"I-TASSER: a unified platform for automated protein structure and function prediction","volume":"5","author":"Roy","year":"2010","journal-title":"Nat. Protoc."},{"key":"2021042015111570600_bib50","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/bies.950190105","article-title":"What does Mos do in oocytes and somatic cells?","volume":"19","author":"Sagata","year":"1997","journal-title":"BioEssays"},{"key":"2021042015111570600_bib51","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1186\/1471-2164-14-923","article-title":"The genome of Romanomermis culicivorax: revealing fundamental changes in the core developmental genetic toolkit in Nematoda","volume":"14","author":"Schiffer","year":"2013","journal-title":"BMC Genomics"},{"key":"2021042015111570600_bib52","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/1471-2105-6-31","article-title":"Automated generation of heuristics for biological sequence comparison","volume":"6","author":"Slater","year":"2005","journal-title":"BMC Bioinformatics"},{"key":"2021042015111570600_bib53","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/S0014-5793(00)02094-9","article-title":"A reversible protein phosphorylation system is present at the surface of infective larvae of the parasitic nematode Trichinella spiralis","volume":"483","author":"Smith","year":"2000","journal-title":"FEBS Lett."},{"key":"2021042015111570600_bib54","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1002\/(SICI)1097-0134(199707)28:3<405::AID-PROT10>3.0.CO;2-L","article-title":"Pfam: a comprehensive database of protein domain families based on seed alignments","volume":"28","author":"Sonnhammer","year":"1997","journal-title":"Proteins"},{"key":"2021042015111570600_bib55","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1186\/s13071-015-1231-5","article-title":"The Haemonchus contortus kinome - a resource for fundamental molecular investigations and drug discovery","volume":"8","author":"Stroehlein","year":"2015","journal-title":"Parasit. Vectors"},{"key":"2021042015111570600_bib56","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1016\/j.str.2014.07.014","article-title":"A plug release mechanism for membrane permeation by MLKL","volume":"22","author":"Su","year":"2014","journal-title":"Structure"},{"key":"2021042015111570600_bib57","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.cell.2011.11.031","article-title":"Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase","volume":"148","author":"Sun","year":"2012","journal-title":"Cell"},{"key":"2021042015111570600_bib58","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nature12031","article-title":"The genomes of four tapeworm species reveal adaptations to parasitism","volume":"496","author":"Tsai","year":"2013","journal-title":"Nature"},{"key":"2021042015111570600_bib59","doi-asserted-by":"crossref","first-page":"e0003788","DOI":"10.1371\/journal.pntd.0003788","article-title":"Pan-phylum comparison of nematode metabolic potential","volume":"9","author":"Tyagi","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"2021042015111570600_bib60","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1038\/nrm2203","article-title":"Mechanisms of specificity in protein phosphorylation","volume":"8","author":"Ubersax","year":"2007","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042015111570600_bib61","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.molcel.2014.03.003","article-title":"Mixed lineage kinase domain-like protein MLKL causes necrotic membrane disruption upon phosphorylation by RIP3","volume":"54","author":"Wang","year":"2014","journal-title":"Mol. Cell"},{"key":"2021042015111570600_bib62","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1038\/cr.2013.91","article-title":"Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis","volume":"23","author":"Wu","year":"2013","journal-title":"Cell Res."},{"key":"2021042015111570600_bib63","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1002\/prot.20264","article-title":"Scoring function for automated assessment of protein structure template quality","volume":"57","author":"Zhang","year":"2004","journal-title":"Proteins"}],"container-title":["G3 Genes|Genomes|Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/g3journal\/article-pdf\/6\/9\/2847\/37184893\/g3journal2847.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/g3journal\/article-pdf\/6\/9\/2847\/37184893\/g3journal2847.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T04:30:49Z","timestamp":1618979449000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/g3journal\/article\/6\/9\/2847\/6027293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,1]]},"references-count":63,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,9,1]]}},"URL":"https:\/\/doi.org\/10.1534\/g3.116.032961","relation":{},"ISSN":["2160-1836"],"issn-type":[{"value":"2160-1836","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2016,9,1]]},"published":{"date-parts":[[2016,9,1]]}}}