{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:50:58Z","timestamp":1783903858475,"version":"3.55.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Wild Helianthus species are a valuable genetic resource for the improvement of cultivated sunflower. We report the discovery and characterization of a unique high frequency production of triploids when cultivated sunflower was pollinated by specific accessions of diploid Helianthus nuttallii T. &amp; G. and H. maximiliani Schr. Genomic in situ hybridization (GISH) analyses indicated that the triploid F1s had two genomes from the wild pollen sources and one from the cultivated line. Mitotic chromosome analyses indicated that the frequency of triploid progenies from the crosses of cultivated lines \u00d7 H. nuttallii accession 102 (N102) was significantly higher than those of unexpected polyploid progenies from the crosses of wild perennial species \u00d7 N102, and no unexpected polyploids were obtained from the reverse crosses. Pollen stainability analysis suggested the existence of a low percentage of unreduced (2n) male gametes in some accessions, especially N102 and H. maximiliani accession 1113 (M1113), which were generated at the telophase II and tetrad stages of meiosis. The triploid F1s could be the results of preferred fertilization of the low frequency of 2n male gametes with the female gametes of the cultivated sunflower, due to the dosage factors related to recognition and rejection of foreign pollen during fertilization. The triploids have been used to produce amphiploids and aneuploids. Future studies of the male gametes\u2019 fate from pollination through fertilization will further uncover the mechanism of this whole genome transmission. Studies of the genetic control of this trait will facilitate research on sunflower polyploidy speciation and evolution, and the utilization of this trait in sunflower breeding.<\/jats:p>","DOI":"10.1534\/g3.116.036327","type":"journal-article","created":{"date-parts":[[2017,2,9]],"date-time":"2017-02-09T01:10:14Z","timestamp":1486602614000},"page":"1097-1108","source":"Crossref","is-referenced-by-count":11,"title":["Triploid Production from Interspecific Crosses of Two Diploid Perennial <i>Helianthus<\/i> with Diploid Cultivated Sunflower (<i>Helianthus annuus<\/i> L.)"],"prefix":"10.1093","volume":"7","author":[{"given":"Zhao","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Plant Sciences, North Dakota State University, Fargo, North Dakota 58108"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald J","family":"Seiler","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture-Agricultural Research Service, Northern Crop Science Laboratory, Fargo, North Dakota 58102"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J","family":"Gulya","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture-Agricultural Research Service, Northern Crop Science Laboratory, Fargo, North Dakota 58102"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiuhuan","family":"Feng","sequence":"additional","affiliation":[{"name":"Plant Science Department, South Dakota State University, Brookings, South Dakota 57007"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid Y","family":"Rashid","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Morden, Manitoba R6M 1Y5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiwen","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences, North Dakota State University, Fargo, North Dakota 58108"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Chien","family":"Jan","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture-Agricultural Research Service, Northern Crop Science Laboratory, Fargo, North Dakota 58102"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,4,1]]},"reference":[{"key":"2021062506595259400_bib1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3109\/10520296909063335","article-title":"Differential staining of aborted and nonaborted pollen.","volume":"44","author":"Alexander","year":"1969","journal-title":"Stain Technol."},{"key":"2021062506595259400_bib2","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1007\/s00299-010-0888-7","article-title":"Recovery of Citrus triploid hybrids by embryo rescue and flow cytometry from 2x \u00d7 2x sexual hybridization and its application to extensive breeding programs.","volume":"29","author":"Aleza","year":"2010","journal-title":"Plant Cell Rep."},{"key":"2021062506595259400_bib3","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1093\/jxb\/erq371","article-title":"Unreduced gamete formation in plants: mechanisms and prospects.","volume":"62","author":"Brownfield","year":"2011","journal-title":"J. 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