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The full extent to which SLC25 family members coregulate heme synthesis with other mitochondrial functions is not clear. In this study, we surveyed 29 nonessential SLC25 family members in Saccharomyces cerevisiae for their ability to support growth in the presence and absence of HEM25. Six SLC25 family members were identified that were required for growth or for heme synthesis in cells lacking Hem25 function. Importantly, we determined that loss of function of the SLC25 family member Flx1, which imports FAD into mitochondria, together with loss of function of Hem25, resulted in inability to grow on media that required yeast cells to supply energy using mitochondrial respiration. We report that specific components of complexes of the electron transport chain are decreased in the absence of Flx1 and Hem25 function. In addition, we show that mitochondria from flx1\u0394 hem25\u0394 cells contain uncharacterized Cox2-containing high molecular weight aggregates. The functions of Flx1 and Hem25 provide a facile explanation for the decrease in heme level, and in specific electron transport chain complex components.<\/jats:p>","DOI":"10.1534\/g3.117.041194","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T01:20:28Z","timestamp":1492046428000},"page":"1861-1873","source":"Crossref","is-referenced-by-count":8,"title":["SLC25 Family Member Genetic Interactions Identify a Role for <i>HEM25<\/i> in Yeast Electron Transport Chain Stability"],"prefix":"10.1093","volume":"7","author":[{"given":"J Noelia","family":"Dufay","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia B3N 0A1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J Pedro","family":"Fern\u00e1ndez-Murray","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia B3N 0A1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher R","family":"McMaster","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia B3N 0A1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,6,1]]},"reference":[{"issue":"1","key":"2021042012251262800_bib1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.bcmd.2006.04.003","article-title":"X-linked sideroblastic anemia associated with a novel ALAS2 mutation and unfortunate skewed X-chromosome inactivation patterns.","volume":"37","author":"Aivado","year":"2006","journal-title":"Blood Cells Mol. 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