{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T07:19:07Z","timestamp":1781939947943,"version":"3.54.5"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Here, we present and characterize the spontaneous X-linked recessive mutation casper, which causes oculocutaneous albinism in threespine sticklebacks (Gasterosteus aculeatus). In humans, Hermansky-Pudlak syndrome results in pigmentation defects due to disrupted formation of the melanin-containing lysosomal-related organelle (LRO), the melanosome. casper mutants display not only reduced pigmentation of melanosomes in melanophores, but also reductions in the iridescent silver color from iridophores, while the yellow pigmentation from xanthophores appears unaffected. We mapped casper using high-throughput sequencing of genomic DNA from bulked casper mutants to a region of the stickleback X chromosome (chromosome 19) near the stickleback ortholog of Hermansky-Pudlak syndrome 5 (Hps5). casper mutants have an insertion of a single nucleotide in the sixth exon of Hps5, predicted to generate an early frameshift. Genome editing using CRISPR\/Cas9 induced lesions in Hps5 and phenocopied the casper mutation. Injecting single or paired Hps5 guide RNAs revealed higher incidences of genomic deletions from paired guide RNAs compared to single gRNAs. Stickleback Hps5 provides a genetic system where a hemizygous locus in XY males and a diploid locus in XX females can be used to generate an easily scored visible phenotype, facilitating quantitative studies of different genome editing approaches. Lastly, we show the ability to better visualize patterns of fluorescent transgenic reporters in Hps5 mutant fish. Thus, Hps5 mutations present an opportunity to study pigmented LROs in the emerging stickleback model system, as well as a tool to aid in assaying genome editing and visualizing enhancer activity in transgenic fish.<\/jats:p>","DOI":"10.1534\/g3.117.1125","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T01:00:21Z","timestamp":1500944421000},"page":"3123-3131","source":"Crossref","is-referenced-by-count":17,"title":["Sequence-Based Mapping and Genome Editing Reveal Mutations in Stickleback<i>Hps5<\/i>Cause Oculocutaneous Albinism and the<i>casper<\/i>Phenotype"],"prefix":"10.1093","volume":"7","author":[{"given":"James C","family":"Hart","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California-Berkeley, California 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig T","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California-Berkeley, California 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,9,1]]},"reference":[{"key":"2021042012430965400_bib1","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1534\/genetics.111.136069","article-title":"Efficient mapping and cloning of mutations in zebrafish by low-coverage whole-genome sequencing.","volume":"190","author":"Bowen","year":"2012","journal-title":"Genetics"},{"key":"2021042012430965400_bib2","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1242\/dev.134809","article-title":"Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes.","volume":"143","author":"Burger","year":"2016","journal-title":"Development"},{"key":"2021042012430965400_bib3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF00571425","article-title":"Physiological color change in squid iridophores. II. Ultrastructural mechanisms in Lolliguncula brevis.","volume":"259","author":"Cooper","year":"1990","journal-title":"Cell Tissue Res."},{"key":"2021042012430965400_bib4","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1073\/pnas.0913203107","article-title":"Identification of MIR390a precursor processing-defective mutants in Arabidopsis by direct genome sequencing.","volume":"107","author":"Cuperus","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042012430965400_bib5","doi-asserted-by":"crossref","first-page":"e1002861","DOI":"10.1371\/journal.pgen.1002861","article-title":"Simple methods for generating and detecting locus-specific mutations induced with TALENs in the zebrafish genome.","volume":"8","author":"Dahlem","year":"2012","journal-title":"PLoS Genet."},{"key":"2021042012430965400_bib6","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1534\/genetics.113.154898","article-title":"snow white, a zebrafish model of Hermansky-Pudlak syndrome type 5.","volume":"195","author":"Daly","year":"2013","journal-title":"Genetics"},{"key":"2021042012430965400_bib7","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.ceb.2004.05.001","article-title":"The building BLOC(k)s of lysosomes and related organelles.","volume":"16","author":"Dell\u2019Angelica","year":"2004","journal-title":"Curr. Opin. Cell Biol."},{"key":"2021042012430965400_bib8","first-page":"1265","article-title":"Lysosome-related organelles.","volume":"14","author":"Dell\u2019Angelica","year":"2000","journal-title":"FASEB J."},{"key":"2021042012430965400_bib9","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/ng.806","article-title":"A framework for variation discovery and genotyping using next-generation DNA sequencing data.","volume":"43","author":"DePristo","year":"2011","journal-title":"Nat. Genet."},{"key":"2021042012430965400_bib10","doi-asserted-by":"crossref","first-page":"e15435","DOI":"10.1371\/journal.pone.0015435","article-title":"C. elegans mutant identification with a one-step whole-genome-sequencing and SNP mapping strategy.","volume":"5","author":"Doitsidou","year":"2010","journal-title":"PLoS One"},{"key":"2021042012430965400_bib11","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.ydbio.2015.02.006","article-title":"A 190 base pair, TGF-\u03b2 responsive tooth and fin enhancer is required for stickleback Bmp6 expression.","volume":"401","author":"Erickson","year":"2015","journal-title":"Dev. Biol."},{"key":"2021042012430965400_bib12","article-title":"Microinjection for transgenesis and genome editing in threespine sticklebacks.","volume":"111","author":"Erickson","year":"2016","journal-title":"J. Vis. Exp."},{"key":"2021042012430965400_bib13","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/j.1600-0854.2006.00514.x","article-title":"The Drosophila pigmentation gene pink (p) encodes a homologue of human Hermansky\u2013Pudlak syndrome 5 (HPS5).","volume":"8","author":"Falc\u00f3n-P\u00e9rez","year":"2007","journal-title":"Traffic"},{"key":"2021042012430965400_bib14","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1038\/nmeth.2532","article-title":"Heritable genome editing in C. elegans via a CRISPR-Cas9 system.","volume":"10","author":"Friedland","year":"2013","journal-title":"Nat. Methods"},{"key":"2021042012430965400_bib15","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1034\/j.1600-0749.2000.130502.x","article-title":"The regulation of motile activity in fish chromatophores.","volume":"13","author":"Fujii","year":"2000","journal-title":"Pigment Cell Res."},{"key":"2021042012430965400_bib16","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s10709-012-9694-1","article-title":"A homolog of the human Hermansky-Pudluck syndrome-5 (HPS5) gene is responsible for the oa larval translucent mutants in the silkworm, Bombyx mori.","volume":"140","author":"Fujii","year":"2012","journal-title":"Genetica"},{"key":"2021042012430965400_bib17","doi-asserted-by":"crossref","first-page":"1890","DOI":"10.1126\/science.1109835","article-title":"The synthesis and evolution of a supermodel.","volume":"307","author":"Gibson","year":"2005","journal-title":"Science"},{"key":"2021042012430965400_bib18","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/2041-9139-5-19","article-title":"Parallel developmental genetic features underlie stickleback gill raker evolution.","volume":"5","author":"Glazer","year":"2014","journal-title":"EvoDevo"},{"key":"2021042012430965400_bib19","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1534\/g3.115.017905","article-title":"Genome assembly improvement and mapping convergently evolved skeletal traits in sticklebacks with genotyping-by-sequencing.","volume":"5","author":"Glazer","year":"2015","journal-title":"G3 (Bethesda)"},{"key":"2021042012430965400_bib20","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1242\/dev.099853","article-title":"Efficient RNA\/Cas9-mediated genome editing in Xenopus tropicalis.","volume":"141","author":"Guo","year":"2014","journal-title":"Development"},{"key":"2021042012430965400_bib21","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1038\/sj.jid.5700737","article-title":"Improper trafficking of melanocyte-specific proteins in Hermansky-Pudlak syndrome type-5.","volume":"127","author":"Helip-Wooley","year":"2007","journal-title":"J. Invest. Dermatol."},{"key":"2021042012430965400_bib22","doi-asserted-by":"crossref","first-page":"10915","DOI":"10.1073\/pnas.89.22.10915","article-title":"Amino acid substitution matrices from protein blocks.","volume":"89","author":"Henikoff","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042012430965400_bib23","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.devcel.2016.02.015","article-title":"Precise editing of the zebrafish genome made simple and efficient.","volume":"36","author":"Hoshijima","year":"2016","journal-title":"Dev. Cell"},{"key":"2021042012430965400_bib24","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1111\/j.1600-0854.2004.00208.x","article-title":"Cellular, molecular and clinical characterization of patients with Hermansky\u2013Pudlak syndrome type 5.","volume":"5","author":"Huizing","year":"2004","journal-title":"Traffic"},{"key":"2021042012430965400_bib25","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nbt.2501","article-title":"Efficient genome editing in zebrafish using a CRISPR-Cas system.","volume":"31","author":"Hwang","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"2021042012430965400_bib26","doi-asserted-by":"crossref","first-page":"4827","DOI":"10.1242\/dev.115584","article-title":"Precise and efficient genome editing in zebrafish using the CRISPR\/Cas9 system.","volume":"141","author":"Irion","year":"2014","journal-title":"Development"},{"key":"2021042012430965400_bib27","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/bs.ctdb.2015.12.012","article-title":"The developmental genetics of vertebrate color pattern formation: lessons from zebrafish.","volume":"117","author":"Irion","year":"2016","journal-title":"Curr. Top. Dev. Biol."},{"key":"2021042012430965400_bib28","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/978-1-4939-2444-8_13","article-title":"QTL mapping using high-throughput sequencing.","volume":"1284","author":"Jamann","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"2021042012430965400_bib29","doi-asserted-by":"crossref","first-page":"e00471","DOI":"10.7554\/eLife.00471","article-title":"RNA-programmed genome editing in human cells.","volume":"2","author":"Jinek","year":"2013","journal-title":"ELife"},{"key":"2021042012430965400_bib30","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nature10944","article-title":"The genomic basis of adaptive evolution in threespine sticklebacks.","volume":"484","author":"Jones","year":"2012","journal-title":"Nature"},{"key":"2021042012430965400_bib31","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1534\/g3.116.036723","article-title":"Efficient screening of CRISPR\/Cas9-induced events in Drosophila using a co-CRISPR strategy.","volume":"7","author":"Kane","year":"2017","journal-title":"G3 (Bethesda)"},{"key":"2021042012430965400_bib32","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1002\/dvdy.20516","article-title":"Transposon tools and methods in zebrafish.","volume":"234","author":"Kawakami","year":"2005","journal-title":"Dev. Dyn."},{"key":"2021042012430965400_bib33","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1111\/j.1600-0749.2004.00174.x","article-title":"Genetics and evolution of pigment patterns in fish.","volume":"17","author":"Kelsh","year":"2004","journal-title":"Pigment Cell Res."},{"key":"2021042012430965400_bib34","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.semcdb.2008.10.001","article-title":"Stripes and belly-spots\u2014a review of pigment cell morphogenesis in vertebrates.","volume":"20","author":"Kelsh","year":"2009","journal-title":"Semin. Cell Dev. Biol."},{"key":"2021042012430965400_bib35","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1534\/genetics.114.166389","article-title":"A co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans.","volume":"197","author":"Kim","year":"2014","journal-title":"Genetics"},{"key":"2021042012430965400_bib36","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2.","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"2021042012430965400_bib37","first-page":"203","article-title":"Slipped-strand mispairing: a major mechanism for DNA sequence evolution.","volume":"4","author":"Levinson","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"2021042012430965400_bib38","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The sequence alignment\/map format and SAMtools.","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"2021042012430965400_bib39","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1038\/35096009","article-title":"The melanosome: membrane dynamics in black and white.","volume":"2","author":"Marks","year":"2001","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042012430965400_bib40","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.cub.2015.11.021","article-title":"CRISPR\/Cas9 mutagenesis reveals versatile roles of Hox genes in crustacean limb specification and evolution.","volume":"26","author":"Martin","year":"2016","journal-title":"Curr. Biol."},{"key":"2021042012430965400_bib41","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1101\/gr.107524.110","article-title":"The genome analysis toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data.","volume":"20","author":"McKenna","year":"2010","journal-title":"Genome Res."},{"key":"2021042012430965400_bib42","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/344330a0","article-title":"Female sticklebacks use male coloration in mate choice and hence avoid parasitized males.","volume":"344","author":"Milinski","year":"1990","journal-title":"Nature"},{"key":"2021042012430965400_bib43","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.semcdb.2008.11.012","article-title":"Not just black and white: pigment pattern development and evolution in vertebrates.","volume":"20","author":"Mills","year":"2009","journal-title":"Semin. Cell Dev. Biol."},{"key":"2021042012430965400_bib44","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1101\/gr.148718.112","article-title":"The origin, evolution, and functional impact of short insertion\u2013deletion variants identified in 179 human genomes.","volume":"23","author":"Montgomery","year":"2013","journal-title":"Genome Res."},{"key":"2021042012430965400_bib45","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.ydbio.2016.08.020","article-title":"no privacy, a Xenopus tropicalis mutant, is a model of human Hermansky-Pudlak syndrome and allows visualization of internal organogenesis during tadpole development.","volume":"426","author":"Nakayama","year":"2017","journal-title":"Dev. Biol."},{"key":"2021042012430965400_bib46","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1046\/j.1523-1747.2002.19535.x","article-title":"Melanosome morphologies in murine models of Hermansky-Pudlak syndrome reflect blocks in organelle development.","volume":"119","author":"Nguyen","year":"2002","journal-title":"J. Invest. Dermatol."},{"key":"2021042012430965400_bib47","doi-asserted-by":"crossref","first-page":"4280","DOI":"10.1242\/dev.083931","article-title":"Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing.","volume":"139","author":"Obholzer","year":"2012","journal-title":"Development"},{"key":"2021042012430965400_bib48","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1034\/j.1600-0749.2001.140502.x","article-title":"Direct reception of light by chromatophores of lower vertebrates.","volume":"14","author":"Oshima","year":"2001","journal-title":"Pigment Cell Res."},{"key":"2021042012430965400_bib49","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/nature17664","article-title":"Efficient introduction of specific homozygous and heterozygous mutations using CRISPR\/Cas9.","volume":"533","author":"Paquet","year":"2016","journal-title":"Nature"},{"key":"2021042012430965400_bib50","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1016\/j.cub.2004.08.030","article-title":"The master sex-determination locus in threespine sticklebacks is on a nascent Y chromosome.","volume":"14","author":"Peichel","year":"2004","journal-title":"Curr. Biol."},{"key":"2021042012430965400_bib51","first-page":"365","article-title":"Primer3 on the WWW for general users and for biologist programmers.","volume":"132","author":"Rozen","year":"2000","journal-title":"Methods Mol. Biol."},{"key":"2021042012430965400_bib52","doi-asserted-by":"crossref","first-page":"W462","DOI":"10.1093\/nar\/gkq319","article-title":"ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool.","volume":"38","author":"Sander","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"2021042012430965400_bib53","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/nmeth0809-550","article-title":"SHOREmap: simultaneous mapping and mutation identification by deep sequencing.","volume":"6","author":"Schneeberger","year":"2009","journal-title":"Nat. Methods"},{"key":"2021042012430965400_bib54","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/j.1600-0749.1999.tb00755.x","article-title":"Current understanding on the role of retinal pigment epithelium and its pigmentation.","volume":"12","author":"Schraermeyer","year":"1999","journal-title":"Pigment Cell Res."},{"key":"2021042012430965400_bib55","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1083\/jcb.29.2.223","article-title":"The development of pigment granules in the eyes of wild type and mutant Drosophila \u00a0melanogaster.","volume":"29","author":"Shoup","year":"1966","journal-title":"J. Cell Biol."},{"key":"2021042012430965400_bib56","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega.","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"2021042012430965400_bib57","doi-asserted-by":"crossref","first-page":"4180","DOI":"10.1242\/dev.125609","article-title":"CRISPR\/Cas9-mediated mutagenesis in the sea lamprey Petromyzon marinus: a powerful tool for understanding ancestral gene functions in vertebrates.","volume":"142","author":"Square","year":"2015","journal-title":"Development"},{"key":"2021042012430965400_bib58","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1139\/G07-032","article-title":"The pink gene encodes the Drosophila orthologue of the human Hermansky-Pudlak syndrome 5 (HPS5) gene.","volume":"50","author":"Syrzycka","year":"2007","journal-title":"Genome"},{"key":"2021042012430965400_bib59","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1089\/zeb.2014.1047","article-title":"A streamlined CRISPR pipeline to reliably generate zebrafish frameshifting alleles.","volume":"11","author":"Talbot","year":"2014","journal-title":"Zebrafish"},{"key":"2021042012430965400_bib60","doi-asserted-by":"crossref","first-page":"11.10.1","DOI":"10.1002\/0471250953.bi1110s43","article-title":"From FastQ data to high confidence variant calls: the genome analysis toolkit best practices pipeline.","volume":"43","author":"Van der Auwera","year":"2013","journal-title":"Curr. Protoc. Bioinformatics"},{"key":"2021042012430965400_bib61","doi-asserted-by":"crossref","first-page":"3995","DOI":"10.1242\/jcs.040667","article-title":"Melanosomes at a glance.","volume":"121","author":"Wasmeier","year":"2008","journal-title":"J. Cell Sci."},{"key":"2021042012430965400_bib62","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1023\/A:1022365315836","article-title":"Different carotenoids and potential information content of red coloration of male three-spined stickleback.","volume":"24","author":"Wedekind","year":"1998","journal-title":"J. Chem. Ecol."},{"key":"2021042012430965400_bib63","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/1346-8138.12025","article-title":"Hypopigmentation in Hermansky\u2013Pudlak syndrome.","volume":"40","author":"Wei","year":"2013","journal-title":"J. Dermatol."},{"key":"2021042012430965400_bib64","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/ng1087","article-title":"Ru2 and Ru encode mouse orthologs of the genes mutated in human Hermansky-Pudlak syndrome types 5 and 6.","volume":"33","author":"Zhang","year":"2003","journal-title":"Nat. Genet."},{"key":"2021042012430965400_bib65","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1034\/j.1600-0749.2003.00044.x","article-title":"The pteridine pathway in zebrafish: regulation and specification during the determination of neural crest cell-fate.","volume":"16","author":"Ziegler","year":"2003","journal-title":"Pigment Cell Res."},{"key":"2021042012430965400_bib66","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1534\/genetics.110.119230","article-title":"A strategy for direct mapping and identification of mutations by whole-genome sequencing.","volume":"186","author":"Zuryn","year":"2010","journal-title":"Genetics"}],"container-title":["G3 Genes|Genomes|Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/g3journal\/article-pdf\/7\/9\/3123\/35315033\/g3journal3123.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/g3journal\/article-pdf\/7\/9\/3123\/35315033\/g3journal3123.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T17:46:20Z","timestamp":1692899180000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/g3journal\/article\/7\/9\/3123\/6027473"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,1]]},"references-count":66,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9,1]]}},"URL":"https:\/\/doi.org\/10.1534\/g3.117.1125","relation":{},"ISSN":["2160-1836"],"issn-type":[{"value":"2160-1836","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2017,9,1]]},"published":{"date-parts":[[2017,9,1]]}}}