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Here we characterize how starvation modulates Caenorhabditis elegans vulval cell fate patterning \u2013 an environmentally sensitive process, with a nevertheless robust output. Past research has shown many vulval mutants affecting EGF-Ras-MAPK, Delta-Notch and Wnt pathways to be suppressed by environmental factors, such as starvation. Here we aimed to resolve previous, seemingly contradictory, observations on how starvation modulates levels of vulval induction. Using the strong starvation suppression of the Vulvaless phenotype of lin-3\/egf reduction-of-function mutations as an experimental paradigm, we first tested for a possible involvement of the sensory system in relaying starvation signals to affect vulval induction: mutation of various sensory inputs, DAF-2\/Insulin or DAF-7\/TGF-\u03b2 signaling did not abolish lin-3(rf) starvation suppression. In contrast, nutrient deprivation induced by mutation of the intestinal peptide transporter gene pept-1 or the TOR pathway component rsks-1 (the ortholog of mammalian P70S6K) very strongly suppressed lin-3(rf) mutant phenotypes. Therefore, physiologically starved animals induced by these mutations tightly recapitulated the effects of external starvation on vulval induction. While both starvation and pept-1 RNAi were sufficient to increase Ras and Notch pathway activities in vulval cells, the highly penetrant Vulvaless phenotype of a tissue-specific null allele of lin-3 was not suppressed by either condition. This and additional results indicate that partial lin-3 expression is required for starvation to affect vulval induction. These results suggest a cross-talk between nutrient deprivation, TOR-S6K and EGF-Ras-MAPK signaling during C. elegans vulval induction.<\/jats:p>","DOI":"10.1534\/g3.118.200449","type":"journal-article","created":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T18:09:44Z","timestamp":1532369384000},"page":"3069-3081","source":"Crossref","is-referenced-by-count":11,"title":["Physiological Starvation Promotes\n                    <i>Caenorhabditis elegans<\/i>\n                    Vulval Induction"],"prefix":"10.1093","volume":"8","author":[{"given":"St\u00e9phanie","family":"Grimbert","sequence":"first","affiliation":[{"name":"Universit\u00e9 C\u00f4te d\u2019Azur, CNRS, Inserm, IBV, Nice, France, 06100 Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amhed Missael","family":"Vargas Velazquez","sequence":"additional","affiliation":[{"name":"Institute de Biologie de l\u2019Ecole Normale Sup\u00e9rieure, CNRS UMR 8197 and Inserm U1024, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Braendle","sequence":"additional","affiliation":[{"name":"Universit\u00e9 C\u00f4te d\u2019Azur, CNRS, Inserm, IBV, Nice, France, 06100 Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,9,1]]},"reference":[{"key":"2021042013341711700_bib1","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1101\/gad.14.12.1512","article-title":"daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans.","volume":"14","author":"Antebi","year":"2000","journal-title":"Genes Dev."},{"key":"2021042013341711700_bib2","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.devcel.2012.12.001","article-title":"Robustness and epistasis in the C. elegans vulval signaling network revealed by pathway dosage modulation.","volume":"24","author":"Barkoulas","year":"2013","journal-title":"Dev. 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