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Like all epithelial cells, tracheal cells use Rab GTPases to organize their internal membrane transport, resulting in the specific localization or secretion of proteins on the apical or basal membrane compartments. Some contributions of Rabs to junctional remodelling and governance of tracheal lumen contents are known, but it is reasonable to assume that they play important further roles in morphogenesis. This pertains in particular to terminal tracheal cells, specialized branch-forming cells that drastically reshape both their apical and basal membrane during the larval stages. We performed a loss-of-function screen in the tracheal system, knocking down endogenously tagged alleles of 26 Rabs by targeting the tag via RNAi. This revealed that at least 14 Rabs are required to ensure proper cell fate specification and migration of the dorsal branches, as well as their epithelial fusion with the contralateral dorsal branch. The screen implicated four Rabs in the subcellular morphogenesis of terminal cells themselves. Further tests suggested residual gene function after knockdown, leading us to discuss the limitations of this approach. We conclude that more Rabs than identified here may be important for tracheal morphogenesis, and that the tracheal system offers great opportunities for studying several Rabs that have barely been characterized so far.<\/jats:p>","DOI":"10.1534\/g3.119.400967","type":"journal-article","created":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T22:15:17Z","timestamp":1579904117000},"page":"1099-1112","source":"Crossref","is-referenced-by-count":12,"title":["Multiple Requirements for Rab GTPases in the Development of\n                    <i>Drosophila<\/i>\n                    Tracheal Dorsal Branches and Terminal Cells"],"prefix":"10.1093","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6965-1117","authenticated-orcid":false,"given":"Benedikt T","family":"Best","sequence":"first","affiliation":[{"name":"Director\u2019s Research Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany and"},{"name":"Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Leptin","sequence":"additional","affiliation":[{"name":"Director\u2019s Research Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,3,1]]},"reference":[{"key":"2021041819533857100_bib1","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1016\/j.str.2015.01.020","article-title":"Structural Analysis of Bacterial ABC Transporter Inhibition by an Antibody Fragment.","volume":"23","author":"Ahuja","year":"2015","journal-title":"Structure"},{"key":"2021041819533857100_bib2","doi-asserted-by":"publisher","first-page":"2335","DOI":"10.1242\/jcs.085613","article-title":"Sequoia establishes tip-cell number in Drosophila trachea by regulating FGF levels.","volume":"124","author":"Araujo","year":"2011","journal-title":"J. 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