{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T17:42:21Z","timestamp":1776015741577,"version":"3.50.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mouse sex chromosomes are enriched for co-amplified gene families, present in tens to hundreds of copies. Co-amplification of Slx\/Slxl1 on the X chromosome and Sly on the Y chromosome are involved in dose-dependent meiotic drive, however the role of other co-amplified genes remains poorly understood. Here we demonstrate that the co-amplified gene family on the X chromosome, Srsx, along with two additional partial gene annotations, is actually part of a larger transcription unit, which we name Laidx. Laidx is harbored in a 229 kb amplicon that represents the ancestral state as compared to a 525 kb Y-amplicon containing the rearranged Laidy. Laidx contains a 25,011 nucleotide open reading frame, predominantly expressed in round spermatids, predicted to encode an 871 kD protein. Laidx has orthologous copies with the rat and also the 825-MY diverged parasitic Chinese liver fluke, Clonorchis sinensis, the likely result of a horizontal gene transfer of rodent Laidx to an ancestor of the liver fluke. To assess the male reproductive functions of Laidx, we generated mice carrying a multi-megabase deletion of the Laidx-ampliconic region. Laidx-deficient male mice do not show detectable reproductive defects in fertility, fecundity, testis histology, and offspring sex ratio. We speculate that Laidx and Laidy represent a now inactive X vs. Y chromosome conflict that occurred in an ancestor of present day mice.<\/jats:p>","DOI":"10.1534\/g3.120.401221","type":"journal-article","created":{"date-parts":[[2020,4,6]],"date-time":"2020-04-06T21:15:21Z","timestamp":1586207721000},"page":"1997-2005","source":"Crossref","is-referenced-by-count":8,"title":["Genomic Structure, Evolutionary Origins, and Reproductive Function of a Large Amplified Intrinsically Disordered Protein-Coding Gene on the X Chromosome (\n                    <i>Laidx<\/i>\n                    ) in Mice"],"prefix":"10.1093","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4716-8295","authenticated-orcid":false,"given":"Martin F","family":"Arlt","sequence":"first","affiliation":[{"name":"Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele A","family":"Brogley","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan R","family":"Stark-Dykema","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueh-Chiang","family":"Hu","sequence":"additional","affiliation":[{"name":"Division of Developmental Biology, Department of Pediatrics, Cincinnati Children\u2019s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob L","family":"Mueller","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,6,1]]},"reference":[{"key":"2021101208453852300_bib1","doi-asserted-by":"publisher","first-page":"D8","DOI":"10.1093\/nar\/gkx1095","article-title":"Database resources of the National Center for Biotechnology Information.","volume":"46","author":"Agarwala","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"2021101208453852300_bib2","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1007\/978-1-60761-103-5_16","article-title":"Staging of mouse seminiferous tubule cross-sections.","volume":"558","author":"Ahmed","year":"2009","journal-title":"Methods Mol. 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