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Though antifungals are available, their effectiveness is dwindling due to resistance. Alternate options that comprise the combination of existing azoles and polyunsaturated fatty acids, such as arachidonic acid (AA), have been shown to increase azoles susceptibility of C. albicans biofilms; however, the mechanisms are still unknown. Therefore, transcriptome analysis was conducted on biofilms exposed to sub-inhibitory concentrations of AA alone, fluconazole alone, and AA combined with fluconazole to understand the possible mechanism involved with the phenomenon. Protein ANalysis THrough Evolutionary Relationships (PANTHER) analysis from the differentially expressed genes revealed that the combination of AA and fluconazole influences biological processes associated with essential processes including methionine synthesis and those involved in ATP generation, such as AMP biosynthesis, fumarate metabolism and fatty acid oxidation. These observations suggests that the interference of AA with these processes may be a possible mechanisms to induce increased antifungal susceptibility.<\/jats:p>","DOI":"10.1534\/g3.120.401340","type":"journal-article","created":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T02:25:15Z","timestamp":1594088715000},"page":"3099-3108","source":"Crossref","is-referenced-by-count":15,"title":["Transcriptome Analyses of <i>Candida albicans<\/i> Biofilms, Exposed to Arachidonic Acid and Fluconazole, Indicates Potential Drug Targets"],"prefix":"10.1093","volume":"10","author":[{"given":"Oluwasegun","family":"Kuloyo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruan","family":"Fourie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Errol","family":"Cason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacobus","family":"Albertyn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina H","family":"Pohl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"2021042106152019900_bib1","doi-asserted-by":"publisher","first-page":"8494","DOI":"10.1021\/bi900578y","article-title":"Fluconazole Modulates Membrane Rigidity, Heterogeneity, and Water Penetration into the Plasma Membrane in Saccharomyces cerevisiae.","volume":"48","author":"Abe","year":"2009","journal-title":"Biochemistry"},{"key":"2021042106152019900_bib2","doi-asserted-by":"publisher","first-page":"e1000217","DOI":"10.1371\/journal.ppat.1000217","article-title":"The Hyphal-Associated Adhesin and Invasin Als3 of Candida albicans Mediates Iron Acquisition from Host Ferritin.","volume":"4","author":"Almeida","year":"2008","journal-title":"PLoS Pathog."},{"key":"2021042106152019900_bib3","unstructured":"Andrews, S. 2010\u2003FastQC: A quality control tool for high throughput sequence data. 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