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Here we identify a novel role for one conserved proneural factor, the bHLH protein HLH-3, implicated in the specification of sex-specific ventral cord motor neurons in C. elegans. Proneural genes act in early stages of neurogenesis in early progenitors, but here, we demonstrate a later role for hlh-3. First, we document that differentiation of the ventral cord type C motor neuron class (VC) within their neuron class, is dynamic in time and space. Expression of VC class-specific and subclass-specific identity genes is distinct through development and is dependent on the VC position along the A-P axis and their proximity to the vulva. Our characterization of the expression of VC class and VC subclass-specific differentiation markers in the absence of hlh-3 function reveals that VC fate specification, differentiation, and morphology requires hlh-3 function. Finally, we conclude that hlh-3 cell-autonomously specifies VC cell fate.<\/jats:p>","DOI":"10.1534\/g3.120.401458","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T23:25:13Z","timestamp":1600989913000},"page":"4201-4213","source":"Crossref","is-referenced-by-count":5,"title":["The Conserved ASCL1\/MASH-1 Ortholog HLH-3 Specifies Sex-Specific Ventral Cord Motor Neuron Fate in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1093","volume":"10","author":[{"given":"Lillian M","family":"Perez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aixa","family":"Alfonso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,11,1]]},"reference":[{"key":"2021042011174188900_bib1","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1126\/science.8342028","article-title":"The Caenorhabditis elegans unc-17 Gene: A Putative Vesicular Acetylcholine Transporter.","volume":"261","author":"Alfonso","year":"1993","journal-title":"Science"},{"key":"2021042011174188900_bib2","first-page":"505","article-title":"Transcriptional selectors, masters, and combinatorial codes: regulatory principles of neural subtype specification. 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