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Therefore, a research strategy was designed to improve our understanding of the mechanisms for maintaining fungal cell wall integrity, and to identify potential targets for new drugs that modulate the underlying protein-protein interactions in Saccharomyces cerevisiae. This study defines roles for Wsc2p and Wsc3p and their interacting protein partners in the cell wall integrity signaling and cell survival mechanisms that respond to treatments with fluconazole and hydrogen peroxide. By combined genetic and biochemical approaches, we report the discovery of 12 novel protein interactors of Wsc2p and Wsc3p. Of these, Wsc2p interacting partners Gtt1p and Yck2p, have opposing roles in the resistance and sensitivity to fluconazole treatments respectively. The interaction of Wsc2p with Ras2p was confirmed by iMYTH and IP-MS approaches and is shown to play a dominant role in response to oxidative stress induced by hydrogen peroxide. Consistent with an earlier study, Ras2p was also identified as an interacting partner of Wsc1p and Mid2p cell wall integrity signaling proteins. Collectively, this study expands the interaction networks of the mechanosensory proteins of the Cell Wall Integrity pathway.<\/jats:p>","DOI":"10.1534\/g3.120.401468","type":"journal-article","created":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T03:15:12Z","timestamp":1594264512000},"page":"3121-3135","source":"Crossref","is-referenced-by-count":10,"title":["Protein Interactions of the Mechanosensory Proteins Wsc2 and Wsc3 for Stress Resistance in <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1093","volume":"10","author":[{"given":"Vladimir","family":"V\u00e9lez-Segarra","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahily","family":"Gonz\u00e1lez-Crespo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ednalise","family":"Santiago-Cartagena","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis E","family":"V\u00e1zquez-Qui\u00f1ones","sequence":"additional","affiliation":[{"name":"School of Science and Technology, University Ana G. Mendez, Cupey Campus, Ana G Mendez Ave, No.1399, San Juan, PR 00926"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelson","family":"Mart\u00ednez-Mat\u00edas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamirelis","family":"Otero","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juli\u00e1n J","family":"Zayas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Siaca","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanmadi","family":"del Rosario","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inoushka","family":"Mej\u00edas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 J","family":"Aponte","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelani C","family":"Collazo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco J","family":"Lasso","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Snider","sequence":"additional","affiliation":[{"name":"Donnelly Centre, Department of Biochemistry, and Department of Molecular Genetics, University of Toronto, Ontario M5S 3E1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Jessulat","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Regina, Regina, Saskatchewan S4S 0A2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Regina, Regina, Saskatchewan S4S 0A2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian C","family":"Rymond","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Kentucky, Lexington, KY 40506"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohan","family":"Babu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Regina, Regina, Saskatchewan S4S 0A2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Stagljar","sequence":"additional","affiliation":[{"name":"Donnelly Centre, Department of Biochemistry, and Department of Molecular Genetics, University of Toronto, Ontario M5S 3E1, Canada"},{"name":"Mediterranean Institute for Life Sciences, Split, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 R","family":"Rodr\u00edguez-Medina","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR 00936-067"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"2021042106150511100_bib1","first-page":"195","article-title":"Opportunistic invasive fungal infections: diagnosis & clinical management.","volume":"139","author":"Badiee","year":"2014","journal-title":"Indian J. 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