{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:09:30Z","timestamp":1791497370677,"version":"4.3.4"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Despite over 100 years of study, the location of the fully sex-linked region of the guppy (Poecilia reticulata) carrying the male-determining locus, and the regions where the XY pair recombine, remain unclear. Previous population genomics studies to determine these regions used small samples from recently bottlenecked captive populations, which increase the false positive rate of associations between individuals\u2019 sexes and SNPs. Using new data from multiple natural populations, we show that a recently proposed candidate for this species\u2019 male-determining gene is probably not completely sex-linked, leaving the maleness factor still unidentified. Variants in the chromosome 12 region carrying the candidate gene sometimes show linkage disequilibrium with the sex-determining factor, but no consistently male-specific variant has yet been found. Our genetic mapping with molecular markers spread across chromosome 12 confirms that this is the guppy XY pair. We describe two families with recombinants between the X and Y chromosomes, which confirm that the male-determining locus is in the region identified by all previous studies, near the terminal pseudo-autosomal region (PAR), which crosses over at a very high rate in males. We correct the PAR marker order, and assign two unplaced scaffolds to the PAR. We also detect a duplication, with one copy in the male-determining region, explaining signals of sex linkage in a more proximal region.<\/jats:p>","DOI":"10.1534\/g3.120.401573","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T22:58:00Z","timestamp":1596581880000},"page":"3639-3649","source":"Crossref","is-referenced-by-count":30,"title":["Locating the Sex Determining Region of Linkage Group 12 of Guppy (\n                    <i>Poecilia reticulata<\/i>\n                    )"],"prefix":"10.1093","volume":"10","author":[{"given":"Deborah","family":"Charlesworth","sequence":"first","affiliation":[{"name":"Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, West Mains Road, EH9 3LF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Bergero","sequence":"additional","affiliation":[{"name":"Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, West Mains Road, EH9 3LF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chay","family":"Graham","sequence":"additional","affiliation":[{"name":"University of Cambridge, Department of Biochemistry, Sanger Building, 80 Tennis Ct Rd, Cambridge CB2 1GA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Gardner","sequence":"additional","affiliation":[{"name":"Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, West Mains Road, EH9 3LF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lengxob","family":"Yong","sequence":"additional","affiliation":[{"name":"Centre for Ecology and Conservation, University of Exeter, Penryn, Falmouth, Cornwall, TR10 9FE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,10,1]]},"reference":[{"key":"2021042107444966400_bib1","doi-asserted-by":"publisher","DOI":"10.1101\/2020.07.13.200196","article-title":"Divergence and remarkable diversity of the Y chromosome in guppies.","author":"Almeida","year":"2020","journal-title":"bioRxiv"},{"key":"2021042107444966400_bib2","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1534\/genetics.114.164293","article-title":"A RAD-Tag genetic map for the Platyfish (Xiphophorus maculatus) reveals mechanisms of karyotype evolution among Teleost fish.","volume":"197","author":"Amores","year":"2014","journal-title":"Genetics"},{"key":"2021042107444966400_bib3","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1038\/nature13206","article-title":"Mammalian Y chromosomes retain widely expressed dosage-sensitive regulators","volume":"508","author":"Bellott","year":"2014","journal-title":"Nature"},{"key":"2021042107444966400_bib4","doi-asserted-by":"publisher","first-page":"6924","DOI":"10.1073\/pnas.1818486116","article-title":"Exaggerated heterochiasmy in a fish with sex-linked male coloration polymorphisms.","volume":"116","author":"Bergero","year":"2019","journal-title":"Proc. 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