{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:05:26Z","timestamp":1761523526132},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2004,6,1]],"date-time":"2004-06-01T00:00:00Z","timestamp":1086048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Retinoblastoma (Rb)\/E2F complexes repress expression of many genes important for G1-to-S transition, but also appear to regulate gene expression at other stages of the cell cycle. In C. elegans, lin-35\/Rb and other synthetic Multivulva (SynMuv) group B genes function redundantly with other sets of genes to regulate G1\/S progression, vulval and pharyngeal differentiation, and other unknown processes required for viabilty. Here we show that lin-35\/Rb, efl-1\/E2F, and other SynMuv B genes negatively regulate a component of the anaphase-promoting complex or cyclosome (APC\/C). The APC\/C is a multisubunit complex that promotes metaphase-to-anaphase progression and G1 arrest by targeting different substrates for ubiquitination and proteasome-mediated destruction. The C. elegans APC\/C gene mat-3\/APC8 has been defined by temperature-sensitive embryonic lethal alleles that strongly affect germline meiosis and mitosis but only weakly affect somatic development. We describe severe nonconditional mat-3 alleles and a hypomorphic viable allele (ku233), all of which affect postembryonic cell divisions including those of the vulval lineage. The ku233 lesion is located outside of the mat-3 coding region and reduces mat-3 mRNA expression. Loss-of-function alleles of lin-35\/Rb and other SynMuv B genes suppress mat-3(ku233) defects by restoring mat-3 mRNA to wild-type levels. Therefore, Rb\/E2F complexes appear to repress mat-3 expression.<\/jats:p>","DOI":"10.1534\/genetics.103.026021","type":"journal-article","created":{"date-parts":[[2004,8,2]],"date-time":"2004-08-02T23:56:34Z","timestamp":1091490994000},"page":"663-672","source":"Crossref","is-referenced-by-count":14,"title":["Caenorhabditis elegans lin-35\/Rb, efl-1\/E2F and Other Synthetic Multivulva Genes Negatively Regulate the Anaphase-Promoting Complex Gene mat-3\/APC8"],"prefix":"10.1093","volume":"167","author":[{"given":"David","family":"Garbe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey B","family":"Doto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meera V","family":"Sundaram","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6100"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,6,1]]},"reference":[{"key":"2022010609480697100_BIB1","doi-asserted-by":"crossref","first-page":"4349","DOI":"10.1242\/dev.128.21.4349","volume":"128","year":"2001","journal-title":"Development"},{"key":"2022010609480697100_BIB2","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1016\/S0960-9822(02)00844-8","volume":"12","year":"2002","journal-title":"Curr. 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