{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:45:40Z","timestamp":1789915540716,"version":"4.0.1"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Serotonin (5-HT) stimulates both pharyngeal pumping and egg laying in Caenorhabditis elegans. Four distinct 5-HT receptors have been partially characterized, but little is known about their function in vivo. SER-7 exhibits most sequence identity to the mammalian 5-HT7 receptors and couples to a stimulation of adenyl cyclase when expressed in COS-7 cells. However, many 5-HT7-specific agonists have low affinity for SER-7. 5-HT fails to stimulate pharyngeal pumping and the firing of the MC motorneurons in animals containing the putative ser-7(tm1325) and ser-7(tm1728) null alleles. In addition, although pumping on bacteria is upregulated in ser-7(tm1325) animals, pumping is more irregular. A similar failure to maintain \u201cfast pumping\u201d on bacteria also was observed in ser-1(ok345) and tph-1(mg280) animals that contain putative null alleles of a 5-HT2-like receptor and tryptophan hydroxylase, respectively, suggesting that serotonergic signaling, although not essential for the upregulation of pumping on bacteria, \u201cfine tunes\u201d the process. 5-HT also fails to stimulate egg laying in ser-7(tm1325), ser-1(ok345), and ser-7(tm1325) ser-1(ok345) animals, but only the ser-7 ser-1 double mutants exhibit an Egl phenotype. All of the SER-7 mutant phenotypes are rescued by the expression of full-length ser-7\u2237gfp translational fusions. ser-7\u2237gfp is expressed in several pharyngeal neurons, including the MC, M2, M3, M4, and M5, and in vulval muscle. Interestingly, 5-HT inhibits egg laying and pharyngeal pumping in ser-7 null mutants and the 5-HT inhibition of egg laying, but not pumping, is abolished in ser-7(tm1325);ser-4(ok512) double mutants. Taken together, these results suggest that SER-7 is essential for the 5-HT stimulation of both egg laying and pharyngeal pumping, but that other signaling pathways can probably fulfill similar roles in vivo.<\/jats:p>","DOI":"10.1534\/genetics.105.044495","type":"journal-article","created":{"date-parts":[[2005,10,4]],"date-time":"2005-10-04T00:34:41Z","timestamp":1128386081000},"page":"159-169","source":"Crossref","is-referenced-by-count":162,"title":["SER-7, a<i>Caenorhabditis elegans<\/i>5-HT7-like Receptor, Is Essential for the 5-HT Stimulation of Pharyngeal Pumping and Egg Laying"],"prefix":"10.1093","volume":"172","author":[{"given":"Robert J","family":"Hobson","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606-3390 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera M","family":"Hapiak","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606-3390 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606-3390 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kara L","family":"Buehrer","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia R","family":"Komuniecki","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606-3390 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard W","family":"Komuniecki","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606-3390 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,1,1]]},"reference":[{"key":"2022010818051933600_BIB1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1098\/rstb.1976.0085","volume":"275","year":"1976","journal-title":"Philos. 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