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Previous mutant screens have identified multiple loci that affect this interaction. Some of these loci correspond to known genes, but many bus genes [those with a bacterially unswollen (Bus) mutant phenotype] have yet to be cloned. We employed Mos1 transposon mutagenesis as a means of more rapidly cloning bus genes and identifying new mutants with altered pathogen response. This approach revealed new infection-related roles for two well-characterized and much-studied genes, egl-8 and tax-4. It also allowed the cloning of a known bus gene, bus-17, which encodes a predicted galactosyltransferase, and of a new bus gene, bus-19, which encodes a novel, albeit ancient, protein. The results illustrate advantages and disadvantages of Mos1 transposon mutagenesis in this system.<\/jats:p>","DOI":"10.1534\/genetics.106.060087","type":"journal-article","created":{"date-parts":[[2006,12,7]],"date-time":"2006-12-07T02:11:37Z","timestamp":1165457497000},"page":"681-697","source":"Crossref","is-referenced-by-count":75,"title":["<i>Mos1<\/i>Mutagenesis Reveals a Diversity of Mechanisms Affecting Response of<i>Caenorhabditis elegans<\/i>to the Bacterial Pathogen<i>Microbacterium nematophilum<\/i>"],"prefix":"10.1093","volume":"175","author":[{"given":"Karen","family":"Yook","sequence":"first","affiliation":[{"name":"Genetics Unit , Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Hodgkin","sequence":"additional","affiliation":[{"name":"Genetics Unit , Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,2,1]]},"reference":[{"key":"2023030304585170100_","doi-asserted-by":"crossref","first-page":"16417","DOI":"10.1074\/jbc.M500924200","volume":"280","year":"2005","journal-title":"J. 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