{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T17:19:02Z","timestamp":1775927942653,"version":"3.50.1"},"reference-count":65,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The organs of animal embryos are typically covered with an extracellular matrix (ECM) that must be carefully remodeled as these organs enlarge during post-embryonic growth; otherwise, their shape and functions may be compromised. We previously described the twisting of the Caenorhabditis elegans \u00a0pharynx (here called the Twp phenotype) as a quantitative mutant phenotype that worsens as that organ enlarges during growth. Mutations previously known to cause pharyngeal twist affect membrane proteins with large extracellular domains (DIG-1 and SAX-7), as well as a C. elegans septin (UNC-61). Here we show that two novel alleles of the C. elegans papilin gene, mig-6(et4) and mig-6(sa580), can also cause the Twp phenotype. We also show that overexpression of the ADAMTS protease gene mig-17 can suppress the pharyngeal twist in mig-6 mutants and identify several alleles of other ECM-related genes that can cause or influence the Twp phenotype, including alleles of fibulin (fbl-1), perlecan (unc-52), collagens (cle-1, dpy-7), laminins (lam-1, lam-3), one ADAM protease (sup-17), and one ADAMTS protease (adt-1). The Twp phenotype in C. elegans is easily monitored using light microscopy, is quantitative via measurements of the torsion angle, and reveals that ECM components, metalloproteinases, and ECM attachment molecules are important for this organ to retain its correct shape during post-embryonic growth. The Twp phenotype is therefore a promising experimental system to study ECM remodeling and diseases.<\/jats:p>","DOI":"10.1534\/genetics.110.120519","type":"journal-article","created":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T02:06:51Z","timestamp":1283220411000},"page":"969-982","source":"Crossref","is-referenced-by-count":22,"title":["Genetics of Extracellular Matrix Remodeling During Organ Growth Using the <i>Caenorhabditis elegans<\/i> Pharynx Model"],"prefix":"10.1093","volume":"186","author":[{"given":"Gholamali","family":"Jafari","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology , University of Gothenburg, S-405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Burghoorn","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Nutrition, Karolinska Institute, Center for Biosciences at NOVUM, S-141 83 Huddinge , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehiro","family":"Kawano","sequence":"additional","affiliation":[{"name":"Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Toronto M5G 1X5 , Canada"},{"name":"Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoj","family":"Mathew","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology , University of Gothenburg, S-405 30 Gothenburg, Sweden"},{"name":"Institut de Cie\u0300ncies Foto\u0300niques, 08860 Castelldefels (Barcelona) , Spain and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catarina","family":"Mo\u0308rck","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology , University of Gothenburg, S-405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claes","family":"Axa\u0308ng","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology , University of Gothenburg, S-405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Ailion","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle , Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James H","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle , Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph G","family":"Culotti","sequence":"additional","affiliation":[{"name":"Samuel Lunenfeld Research Institute of Mount Sinai Hospital, Toronto M5G 1X5 , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Swoboda","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Nutrition, Karolinska Institute, Center for Biosciences at NOVUM, S-141 83 Huddinge , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Pilon","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology , University of Gothenburg, S-405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,11,1]]},"reference":[{"key":"2022110711371038600_BIB1","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1083\/jcb.152.6.1219","volume":"152","year":"2001","journal-title":"J. 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