{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:54:45Z","timestamp":1793120085980,"version":"4.5.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Beneficial mutations are required for adaptation to novel environments, yet the range of mutational pathways that are available to a population has been poorly characterized, particularly in eukaryotes. We assessed the genetic changes of the first mutations acquired during adaptation to a novel environment (exposure to the fungicide, nystatin) in 35 haploid lines of Saccharomyces cerevisiae. Through whole-genome resequencing we found that the genomic scope for adaptation was narrow; all adapted lines acquired a mutation in one of four late-acting genes in the ergosterol biosynthesis pathway, with very few other mutations found. Lines that acquired different ergosterol mutations in the same gene exhibited very similar tolerance to nystatin. All lines were found to have a cost relative to wild type in an unstressful environment; the level of this cost was also strongly correlated with the ergosterol gene bearing the mutation. Interestingly, we uncovered both positive and negative effects on tolerance to other harsh environments for mutations in the different ergosterol genes, indicating that these beneficial mutations have effects that differ in sign among environmental challenges. These results demonstrate that although the genomic target was narrow, different adaptive mutations can lead populations down different evolutionary pathways, with respect to their ability to tolerate (or succumb to) other environmental challenges.<\/jats:p>","DOI":"10.1534\/genetics.112.142620","type":"journal-article","created":{"date-parts":[[2012,6,20]],"date-time":"2012-06-20T00:36:35Z","timestamp":1340152595000},"page":"241-252","source":"Crossref","is-referenced-by-count":69,"title":["Parallel Genetic Changes and Nonparallel Gene\u2013Environment Interactions Characterize the Evolution of Drug Resistance in Yeast"],"prefix":"10.1093","volume":"192","author":[{"given":"Aleeza C","family":"Gerstein","sequence":"first","affiliation":[{"name":"Department of Zoology and Biodiversity Research Centre , University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dara S","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Zoology and Biodiversity Research Centre , University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah P","family":"Otto","sequence":"additional","affiliation":[{"name":"Department of Zoology and Biodiversity Research Centre , University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,9,1]]},"reference":[{"key":"2023030722114632100_","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1093\/genetics\/163.4.1287","article-title":"Mode of selection and experimental evolution of antifungal drug resistance in Saccharomyces cerevisiae","volume":"163","author":"Anderson","year":"2003","journal-title":"Genetics"},{"key":"2023030722114632100_","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1186\/1471-2164-11-88","article-title":"Whole-genome sequencing of a laboratory-evolved yeast strain","volume":"11","author":"Araya","year":"2010","journal-title":"BMC Genomics"},{"key":"2023030722114632100_","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1128\/JCM.37.10.3332-3337.1999","article-title":"Quantitation of ergosterol content: novel method for determination of fluconazole susceptibility of Candida albicans","volume":"37","author":"Arthington-Skaggs","year":"1999","journal-title":"J. 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