{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:40:14Z","timestamp":1773297614651,"version":"3.50.1"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and beta-gamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (\u2212\/\u2212) showed no Abpa27 or Abpbg27 transcripts in submandibular gland complementary DNA (cDNA) libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the \u2212\/\u2212 genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands. We also observed a decrease in BG26 protein in the \u2212\/\u2212 animals, suggesting monomer instability. Overall, we observed no major phenotypic changes in the \u2212\/\u2212 genotype, compared with their +\/+ and +\/\u2212 siblings raised in a laboratory setting, including normal growth curves, tissue histology, fecundity, and longevity. The only difference is that male and female C57BL\/6 mice preferred saliva of the opposite sex containing ABP statistically significantly more than saliva of the opposite sex without ABP in a Y-maze test. These results show for the first time that mice can sense the presence of ABP between saliva targets with and without ABPs, and that they spend more time investigating the target containing ABP.<\/jats:p>","DOI":"10.1534\/genetics.116.194571","type":"journal-article","created":{"date-parts":[[2017,2,4]],"date-time":"2017-02-04T02:20:32Z","timestamp":1486174832000},"page":"1517-1527","source":"Crossref","is-referenced-by-count":16,"title":["Studies of an <i>Androgen-Binding Protein<\/i> Knockout Corroborate a Role for Salivary ABP in Mouse Communication"],"prefix":"10.1093","volume":"205","author":[{"given":"Amanda G","family":"Chung","sequence":"first","affiliation":[{"name":"Department of Medicine , College of Medicine, University of Arizona, Tucson, Arizona 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip M","family":"Belone","sequence":"additional","affiliation":[{"name":"Department of Medicine , College of Medicine, University of Arizona, Tucson, Arizona 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbora Vo\u0161lajerov\u00e1","family":"B\u00edmov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics , Academy of Sciences of the Czech Republic, Lib\u011bchov 27721, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C","family":"Karn","sequence":"additional","affiliation":[{"name":"Department of Medicine , College of Medicine, University of Arizona, Tucson, Arizona 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina M","family":"Laukaitis","sequence":"additional","affiliation":[{"name":"Department of Medicine , College of Medicine, University of Arizona, Tucson, Arizona 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,4,1]]},"reference":[{"key":"2022110309200962200_bib1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1074\/jbc.M106678200","article-title":"Characterization and cloning of two isoforms of heteroglobin, a novel heterodimeric glycoprotein of the secretoglobin-uteroglobin family showing tissue-specific and sex differential expression.","volume":"277","author":"Alvarez","year":"2002","journal-title":"J. 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