{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:15:50Z","timestamp":1791296150124,"version":"4.1.0"},"reference-count":62,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The oocyte spindle in most animal species is assembled in the absence of the microtubule-organizing centers called centrosomes. Without the organization provided by centrosomes, acentrosomal meiotic spindle organization may rely heavily on the bundling of microtubules by kinesin motor proteins. Indeed, the minus-end directed kinesin-14 NCD, and the plus-end directed kinesin-6 Subito are known to be required for oocyte spindle organization in Drosophila melanogaster. How multiple microtubule-bundling kinesins interact to produce a functional acentrosomal spindle is not known. In addition, there have been few studies on the meiotic function of one of the most important microtubule-bundlers in mitotic cells, the kinesin-5 KLP61F. We have found that the kinesin-5 KLP61F is required for spindle and centromere symmetry in oocytes. The asymmetry observed in the absence of KLP61F depends on NCD, the kinesin-12 KLP54D, and the microcephaly protein ASP. In contrast, KLP61F and Subito work together in maintaining a bipolar spindle. We propose that the prominent central spindle, stabilized by Subito, provides the framework for the coordination of multiple microtubule-bundling activities. The activities of several proteins, including NCD, KLP54D, and ASP, generate asymmetries within the acentrosomal spindle, while KLP61F and Subito balance these forces, resulting in the capacity to accurately segregate chromosomes.<\/jats:p>","DOI":"10.1534\/genetics.116.194647","type":"journal-article","created":{"date-parts":[[2016,12,8]],"date-time":"2016-12-08T20:15:14Z","timestamp":1481228114000},"page":"517-527","source":"Crossref","is-referenced-by-count":27,"title":["Cooperation Between Kinesin Motors Promotes Spindle Symmetry and Chromosome Organization in Oocytes"],"prefix":"10.1093","volume":"205","author":[{"given":"Sarah J","family":"Radford","sequence":"first","affiliation":[{"name":"Waksman Institute , Rutgers University, Piscataway, New Jersey 08854"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allysa Marie M","family":"Go","sequence":"additional","affiliation":[{"name":"Waksman Institute , Rutgers University, Piscataway, New Jersey 08854"},{"name":"Department of Genetics , Rutgers University, Piscataway, New Jersey 08854"},{"name":"Waksman Institute , Rutgers University, Piscataway, New Jersey 08854"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim S","family":"McKim","sequence":"additional","affiliation":[{"name":"Waksman Institute , Rutgers University, Piscataway, New Jersey 08854"},{"name":"Department of Genetics , Rutgers University, Piscataway, New Jersey 08854"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,2,1]]},"reference":[{"key":"2023030910485088900_","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/BF00710603","article-title":"Segregation of holocentric chromosomes at meiosis in the nematode, Caenorhabditis elegans.","volume":"1","author":"Albertson","year":"1993","journal-title":"Chromosome Res."},{"key":"2023030910485088900_","doi-asserted-by":"crossref","first-page":"D786","DOI":"10.1093\/nar\/gkv1046","article-title":"FlyBase: establishing a gene group resource for Drosophila melanogaster.","volume":"44","author":"Attrill","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"2023030910485088900_","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/0092-8674(95)90142-6","article-title":"Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo.","volume":"83","author":"Blangy","year":"1995","journal-title":"Cell"},{"key":"2023030910485088900_","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1242\/dev.118.2.401","article-title":"Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.","volume":"118","author":"Brand","year":"1993","journal-title":"Development"},{"key":"2023030910485088900_","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1083\/jcb.201005065","article-title":"HURP permits MTOC sorting for robust meiotic spindle bipolarity, similar to extra centrosome clustering in cancer cells.","volume":"191","author":"Breuer","year":"2010","journal-title":"J. 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