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In...<\/jats:p><jats:p>The process of selecting for cellular fitness through competition plays a critical role in both development and disease. The germarium, a structure at the tip of the ovariole of a Drosophila ovary, contains two follicle stem cells (FSCs) that undergo neutral competition for the stem cell niche. Using the FSCs as a model, we performed a genetic screen through a collection of 126 mutants in essential genes on the X chromosome to identify candidates that increase or decrease competition for the FSC niche. We identified \u223c55 and 6% of the mutations screened as putative FSC hypo- or hyper-competitors, respectively. We found that a large majority of mutations in vesicle trafficking genes (11 out of the 13 in the collection of mutants) are candidate hypo-competition alleles, and we confirmed the hypo-competition phenotype for four of these alleles. We also show that Sec16 and another COPII vesicle trafficking component, Sar1, are required for follicle cell differentiation. Lastly, we demonstrate that, although some components of vesicle trafficking are also required for neutral competition in the cyst stem cells of the testis, there are important tissue-specific differences. Our results demonstrate a critical role for vesicle trafficking in stem cell niche competition and differentiation, and we identify a number of putative candidates for further exploration.<\/jats:p>","DOI":"10.1534\/genetics.117.201202","type":"journal-article","created":{"date-parts":[[2017,5,17]],"date-time":"2017-05-17T00:55:25Z","timestamp":1494982525000},"page":"1417-1428","source":"Crossref","is-referenced-by-count":17,"title":["Neutral Competition for<i>Drosophila<\/i>Follicle and Cyst Stem Cell Niches Requires Vesicle Trafficking Genes"],"prefix":"10.1093","volume":"206","author":[{"given":"Matthew S","family":"Cook","sequence":"first","affiliation":[{"name":"Center for Reproductive Sciences , University of California, San Francisco, California 94143-0452"},{"name":"Department of Anatomy , University of California, San Francisco, California 94143-0452"},{"name":"Department of Obstetrics , Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143-0452"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralie","family":"Cazin","sequence":"additional","affiliation":[{"name":"The Helen and Martin Kimmel Center for Stem Cell Biology , New York University School of Medicine, New York 10016"},{"name":"Department of Biochemistry and Molecular Pharmacology , New York University School of Medicine, New York 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Amoyel","sequence":"additional","affiliation":[{"name":"The Helen and Martin Kimmel Center for Stem Cell Biology , New York University School of Medicine, New York 10016"},{"name":"Department of Biochemistry and Molecular Pharmacology , New York University School of Medicine, New York 10016"},{"name":"School of Cellular and Molecular Medicine , University of Bristol, BS8 1TD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinya","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics , Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030"},{"name":"Jan and Dan Duncan Neurological Research Institute , Texas Children\u2019s Hospital, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Bach","sequence":"additional","affiliation":[{"name":"The Helen and Martin Kimmel Center for Stem Cell Biology , New York University School of Medicine, New York 10016"},{"name":"Department of Biochemistry and Molecular Pharmacology , New York University School of Medicine, New York 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd","family":"Nystul","sequence":"additional","affiliation":[{"name":"Center for Reproductive Sciences , University of California, San Francisco, California 94143-0452"},{"name":"Department of Anatomy , University of California, San Francisco, California 94143-0452"},{"name":"Department of Obstetrics , Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143-0452"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,7,1]]},"reference":[{"key":"2023030910510475500_","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.15252\/embj.201387500","article-title":"Neutral competition of stem cells is skewed by proliferative changes downstream of Hh and Hpo.","volume":"33","author":"Amoyel","year":"2014","journal-title":"EMBO J."},{"key":"2023030910510475500_","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/ncb1611","article-title":"Wurst is essential for airway clearance and respiratory-tube size control.","volume":"9","author":"Behr","year":"2007","journal-title":"Nat. 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