{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:40:54Z","timestamp":1790257254240,"version":"4.1.0"},"reference-count":118,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Understanding evolution requires the comparison of more than a few model species, and exploration of the genotype\/phenotype relationship is limited...<\/jats:p>\n                  <jats:p>Mapping-by-sequencing has become a standard method to map and identify phenotype-causing mutations in model species. Here, we show that a fragmented draft assembly is sufficient to perform mapping-by-sequencing in nonmodel species. We generated a draft assembly and annotation of the genome of the free-living nematode Oscheius tipulae, a distant relative of the model Caenorhabditis elegans. We used this draft to identify the likely causative mutations at the O. tipulae cov-3 locus, which affect vulval development. The cov-3 locus encodes the O. tipulae ortholog of C. elegans mig-13, and we further show that Cel-mig-13 mutants also have an unsuspected vulval-development phenotype. In a virtuous circle, we were able to use the linkage information collected during mutant mapping to improve the genome assembly. These results showcase the promise of genome-enabled forward genetics in nonmodel species.<\/jats:p>","DOI":"10.1534\/genetics.117.203521","type":"journal-article","created":{"date-parts":[[2017,6,19]],"date-time":"2017-06-19T21:00:20Z","timestamp":1497906020000},"page":"1747-1761","source":"Crossref","is-referenced-by-count":26,"title":["Toward Universal Forward Genetics: Using a Draft Genome Sequence of the Nematode\n                    <i>Oscheius tipulae<\/i>\n                    To Identify Mutations Affecting Vulva Development"],"prefix":"10.1093","volume":"206","author":[{"given":"Fabrice","family":"Besnard","sequence":"first","affiliation":[{"name":"\u00c9cole Normale Sup\u00e9rieure , Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Institut de Biologie de l\u2019\u00c9cole Normale Sup\u00e9rieure, Paris Sciences et Lettres Research University, 75005, France"},{"name":"\u00c9cole Normale Sup\u00e9rieure , Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Institut de Biologie de l\u2019\u00c9cole Normale Sup\u00e9rieure, Paris Sciences et Lettres Research University, 75005, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Koutsovoulos","sequence":"additional","affiliation":[{"name":"Institute of Evolutionary Biology , University of Edinburgh, EH8 9YL, United Kingdom"},{"name":"\u00c9cole Normale Sup\u00e9rieure , Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Institut de Biologie de l\u2019\u00c9cole Normale Sup\u00e9rieure, Paris Sciences et Lettres Research University, 75005, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Dieudonn\u00e9","sequence":"additional","affiliation":[{"name":"\u00c9cole Normale Sup\u00e9rieure , Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Institut de Biologie de l\u2019\u00c9cole Normale Sup\u00e9rieure, Paris Sciences et Lettres Research University, 75005, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Blaxter","sequence":"additional","affiliation":[{"name":"Institute of Evolutionary Biology , University of Edinburgh, EH8 9YL, United Kingdom"},{"name":"\u00c9cole Normale Sup\u00e9rieure , Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Institut de Biologie de l\u2019\u00c9cole Normale Sup\u00e9rieure, Paris Sciences et Lettres Research University, 75005, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Anne","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"\u00c9cole Normale Sup\u00e9rieure , Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Institut de Biologie de l\u2019\u00c9cole Normale Sup\u00e9rieure, Paris Sciences et Lettres Research University, 75005, France"},{"name":"\u00c9cole Normale Sup\u00e9rieure , Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Institut de Biologie de l\u2019\u00c9cole Normale Sup\u00e9rieure, Paris Sciences et Lettres Research University, 75005, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,8,1]]},"reference":[{"key":"2023030911011005100_","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/nbt.2095","article-title":"Genome sequencing reveals agronomically important loci in rice using MutMap.","volume":"30","author":"Abe","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"2023030911011005100_","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1139\/g06-045","article-title":"The genome of Oscheius tipulae: determination of size, complexity, and structure by DNA reassociation using fluorescent dye.","volume":"49","author":"Ahn","year":"2006","journal-title":"Genome"},{"key":"2023030911011005100_","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0091-679X(08)61382-5","article-title":"Mutagenesis.","volume":"48","author":"Anderson","year":"1995","journal-title":"Methods Cell Biol."},{"key":"2023030911011005100_"},{"key":"2023030911011005100_","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1111\/j.1365-294X.2008.03697.x","article-title":"Oscheius tipulae, a widespread hermaphroditic soil nematode, displays a higher genetic diversity and geographical structure than Caenorhabditis elegans.","volume":"17","author":"Ba\u00eflle","year":"2008","journal-title":"Mol. Ecol."},{"key":"2023030911011005100_","doi-asserted-by":"crossref","first-page":"e3376","DOI":"10.1371\/journal.pone.0003376","article-title":"Rapid SNP discovery and genetic mapping using sequenced RAD markers.","volume":"3","author":"Baird","year":"2008","journal-title":"PLoS One"},{"key":"2023030911011005100_","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing.","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. 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