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Mind Bomb is an essential E3 ligase during Notch pathway signaling in insects and vertebrates. While Caenorhabditis elegans encodes a Mind Bomb homolog (mib-1), it has never been recovered in the extensive Notch suppressor\/enhancer screens that have identified numerous pathway components. Here, we show that C. elegans mib-1 null mutants have a spermatogenesis-defective phenotype that results in a heterogeneous mixture of arrested spermatocytes, defective spermatids, and motility-impaired spermatozoa. mib-1 mutants also have chromosome segregation defects during meiosis, molecular null mutants are intrinsically temperature-sensitive, and many mib-1 spermatids contain large amounts of tubulin. These phenotypic features are similar to the endogenous RNA intereference (RNAi) mutants, but mib-1 mutants do not affect RNAi. MIB-1 protein is expressed throughout the germ line with peak expression in spermatocytes followed by segregation into the residual body during spermatid formation. C. elegans mib-1 expression, while upregulated during spermatogenesis, also occurs somatically, including in vulva precursor cells. Here, we show that mib-1 mutants suppress both lin-12 and glp-1 (C. elegans Notch) gain-of-function mutants, restoring anchor cell formation and a functional vulva to the former and partly restoring oocyte production to the latter. However, suppressed hermaphrodites are only observed when grown at 25\u00b0, and they are self-sterile. This probably explains why mib-1 was not previously recovered as a Notch pathway component in suppressor\/enhancer selection experiments.<\/jats:p>","DOI":"10.1534\/genetics.118.300807","type":"journal-article","created":{"date-parts":[[2018,3,13]],"date-time":"2018-03-13T00:40:22Z","timestamp":1520901622000},"page":"173-193","source":"Crossref","is-referenced-by-count":10,"title":["MIB-1 Is Required for Spermatogenesis and Facilitates LIN-12 and GLP-1 Activity in<i>Caenorhabditis elegans<\/i>"],"prefix":"10.1093","volume":"209","author":[{"given":"Miriam","family":"Ratliff","sequence":"first","affiliation":[{"name":"Department of Biology, Emory University, Atlanta, Georgia 30322"},{"name":"Department of Biology, Emory University, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine L","family":"Hill-Harfe","sequence":"additional","affiliation":[{"name":"Department of Biology, Emory University, Atlanta, Georgia 30322"},{"name":"Program in Genetics and Molecular Biology, Graduate Division of Biological and Biomedical Sciences, Emory University, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth J","family":"Gleason","sequence":"additional","affiliation":[{"name":"Department of Biology, Emory University, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiping","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Biology, Emory University, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim L","family":"Kroft","sequence":"additional","affiliation":[{"name":"Department of Biology, Emory University, Atlanta, Georgia 30322"},{"name":"Department of Biology, Emory University, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven W","family":"L\u2019Hernault","sequence":"additional","affiliation":[{"name":"Department of Biology, Emory University, Atlanta, Georgia 30322"},{"name":"Program in Genetics and Molecular Biology, Graduate Division of Biological and Biomedical Sciences, Emory University, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,3,12]]},"reference":[{"key":"2022011723191307000_bib1","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1016\/j.cell.2007.12.016","article-title":"Rumi is a CAP10 domain glycosyltransferase that modifies Notch and is required for Notch signaling.","volume":"132","author":"Acar","year":"2008","journal-title":"Cell"},{"key":"2022011723191307000_bib2","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/S0091-679X(08)61382-5","article-title":"Mutagenesis","volume-title":"Methods in Cell Biology. 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