{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T06:33:14Z","timestamp":1776321194288,"version":"3.50.1"},"reference-count":72,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, Schumann et al. identify the penta-EF-hand protein PEF1 of the genetic model fungus Neurospora crassa as part of the cellular response to different types of membrane injury...<\/jats:p><jats:p>Plasma membrane damage commonly occurs during cellular growth and development. To counteract these potentially lethal injuries, membrane repair mechanisms have evolved, which promote the integrity of the lipid bilayer. Although the membrane of fungi is the target of important clinical drugs and agricultural fungicides, the molecular mechanisms which mediate membrane repair in these organisms remain elusive. Here we identify the penta-EF-hand protein PEF1 of the genetic model fungus Neurospora crassa as part of a cellular response mechanism against different types of membrane injury. Deletion of the pef1 gene in the wild type and different lysis-prone gene knockout mutants revealed a function of the protein in maintaining cell integrity during cell\u2013cell fusion and in the presence of pore-forming drugs, such as the plant defense compound tomatine. By fluorescence and live-cell imaging we show that green fluorescent protein (GFP)-tagged PEF1 accumulates at the sites of membrane injury in a Ca2+-dependent manner. Site-directed mutagenesis identified Ca2+-binding domains essential for the spatial dynamics and function of the protein. In addition, the subcellular localization of PEF1 revealed that the syncytial fungal colony undergoes compartmentation in response to antifungal treatment. We propose that plasma membrane repair in fungi constitutes an additional line of defense against membrane-disturbing drugs, thereby expanding the current model of fungal drug resistance mechanisms.<\/jats:p>","DOI":"10.1534\/genetics.119.302363","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T01:45:33Z","timestamp":1562204733000},"page":"195-211","source":"Crossref","is-referenced-by-count":10,"title":["Plasma Membrane Integrity During Cell\u2013Cell Fusion and in Response to Pore-Forming Drugs Is Promoted by the Penta-EF-Hand Protein PEF1 in<i>Neurospora crassa<\/i>"],"prefix":"10.1093","volume":"213","author":[{"given":"Marcel Ren\u00e9","family":"Schumann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Brandt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Adis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Hartung","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Genetik , Technische Universit\u00e4t Braunschweig, 38106, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9","family":"Flei\u00dfner","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Genetik , Technische Universit\u00e4t Braunschweig, 38106, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,9,1]]},"reference":[{"key":"2023031005253846400_","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1091\/mbc.e06-09-0776","article-title":"The plasma membrane proteins Prm1 and Fig1 ascertain fidelity of membrane fusion during yeast mating.","volume":"18","author":"Aguilar","year":"2007","journal-title":"Mol. 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