{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:41:08Z","timestamp":1785436868116,"version":"3.56.0"},"reference-count":82,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Dr osophila Lobe (L) alleles were first discovered \u223c100\u00a0years ago as spontaneous dominant mutants with characteristic developmental eye defects. However, the molecular basis for L dominant eye phenotypes has not been clearly understood. A previous work reported identification of CG10109\/PRAS40 as the L gene, but subsequent analyses suggested that PRAS40 may not be related to L. Here, we revisited the L gene to clarify this discrepancy and understand the basis for the dominance of L mutations. Genetic analysis localized the L gene to Oaz, which encodes a homolog of the vertebrate zinc finger protein 423 (Zfp423) family transcriptional regulators. We demonstrate that RNAi knockdown of Oaz almost completely restores all L dominant alleles tested. Lrev6-3, a revertant allele of the L2 dominant eye phenotype, has an inframe deletion in the Oaz coding sequence. Molecular analysis of L dominant mutants identified allele-specific insertions of natural transposons (roo[ ]L1, hopper[ ]L5, and roo[ ]Lr) or alterations of a preexisting transposon (L2-specific mutations in roo[ ]Mohr) in the Oaz region. In addition, we generated additional L2-reversion alleles by CRISPR targeting at Oaz. These new loss-of-function Oaz mutations suppress the dominant L eye phenotype. Oaz protein is not expressed in wild-type eye disc but is expressed ectopically in L2\/+ mutant eye disc. We induced male recombination between Oaz-GAL4 insertions and the L2 mutation through homologous recombination. By using the L2-recombined GAL4 reporters, we show that Oaz-GAL4 is expressed ectopically in L2 eye imaginal disc. Taken together, our data suggest that neomorphic L eye phenotypes are likely due to misregulation of Oaz by spontaneous transposon insertions.<\/jats:p>","DOI":"10.1534\/genetics.120.303486","type":"journal-article","created":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T01:45:16Z","timestamp":1594259116000},"page":"117-134","source":"Crossref","is-referenced-by-count":3,"title":["The Classic <i>Lobe<\/i> Eye Phenotype of <i>Drosophila<\/i> Is Caused by Transposon Insertion-Induced Misexpression of a Zinc-Finger Transcription Factor"],"prefix":"10.1093","volume":"216","author":[{"given":"Wonseok","family":"Son","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Wook","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"2021050609175431700_bib1","doi-asserted-by":"publisher","first-page":"3076","DOI":"10.1128\/MCB.00185-14","article-title":"Direct transcriptional repression of Zfp423 by Zfp521 mediates a bone morphogenic protein-dependent osteoblast vs. adipocyte lineage commitment switch.","volume":"34","author":"Addison","year":"2014","journal-title":"Mol. Cell. Biol."},{"key":"2021050609175431700_bib2","doi-asserted-by":"publisher","first-page":"19424","DOI":"10.1073\/pnas.0609184103","article-title":"Zfp423 controls proliferation and differentiation of neural precursors in cerebellar vermis formation.","volume":"103","author":"Alcaraz","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021050609175431700_bib3","first-page":"161","article-title":"An eye imaginal disc-specific transcriptional enhancer in the long terminal repeat of the tom retrotransposon is responsible for eye morphology mutations of Drosophila ananassae.","volume":"251","author":"Awasaki","year":"1996","journal-title":"Mol. Gen. Genet."},{"key":"2021050609175431700_bib4","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/978-1-59745-583-1_4","article-title":"The use of P-element transposons to generate transgenic flies.","volume":"420","author":"Bachmann","year":"2008","journal-title":"Methods Mol. Biol."},{"key":"2021050609175431700_bib5","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1093\/genetics\/94.2.383","article-title":"Sex and the single cell. I. On the action of major loci affecting sex determination in Drosophila melanogaster.","volume":"94","author":"Baker","year":"1980","journal-title":"Genetics"},{"key":"2021050609175431700_bib6","doi-asserted-by":"publisher","first-page":"8026","DOI":"10.1073\/pnas.84.22.8026","article-title":"Sexual behavior: its genetic control during development and adulthood in Drosophila melanogaster.","volume":"84","author":"Belote","year":"1987","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021050609175431700_bib7","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1093\/genetics\/139.2.631","article-title":"Transposon insertions causing constitutive Sex-lethal activity in Drosophila melanogaster affect Sxl sex-specific transcript splicing.","volume":"139","author":"Bernstein","year":"1995","journal-title":"Genetics"},{"key":"2021050609175431700_bib8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1534\/genetics.117.1113","article-title":"Advances in engineering the fly genome with the CRISPR-Cas system.","volume":"208","author":"Bier","year":"2018","journal-title":"Genetics"},{"key":"2021050609175431700_bib9","doi-asserted-by":"publisher","first-page":"848","DOI":"10.1016\/j.biocel.2007.04.006","article-title":"Early hematopoietic zinc finger protein-zinc finger protein 521: a candidate regulator of diverse immature cells.","volume":"40","author":"Bond","year":"2008","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"2021050609175431700_bib10","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1186\/s13059-018-1577-z","article-title":"Ten things you should know about transposable elements.","volume":"19","author":"Bourque","year":"2018","journal-title":"Genome Biol."},{"key":"2021050609175431700_bib11","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1006\/dbio.1998.9136","article-title":"The dominant mutation Glazed is a gain-of-function allele of wingless that, similar to loss of APC, interferes with normal eye development.","volume":"206","author":"Brunner","year":"1999","journal-title":"Dev. Biol."},{"key":"2021050609175431700_bib12","doi-asserted-by":"publisher","first-page":"740","DOI":"10.1016\/j.cell.2012.04.019","article-title":"Human transposon tectonics.","volume":"149","author":"Burns","year":"2012","journal-title":"Cell"},{"key":"2021050609175431700_bib13","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1016\/0092-8674(95)90497-2","article-title":"phyllopod functions in the fate determination of a subset of photoreceptors in Drosophila.","volume":"80","author":"Chang","year":"1995","journal-title":"Cell"},{"key":"2021050609175431700_bib14","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.molcel.2012.07.029","article-title":"Playing the end game: DNA double-strand break repair pathway choice.","volume":"47","author":"Chapman","year":"2012","journal-title":"Mol. Cell"},{"key":"2021050609175431700_bib15","doi-asserted-by":"crossref","first-page":"4005","DOI":"10.1242\/dev.129.17.4005","article-title":"Lobe mediates Notch signaling to control domain-specific growth in the Drosophila eye disc.","volume":"129","author":"Chern","year":"2002","journal-title":"Development"},{"key":"2021050609175431700_bib16","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1038\/nrg.2016.139","article-title":"Regulatory activities of transposable elements: from conflicts to benefits.","volume":"18","author":"Chuong","year":"2017","journal-title":"Nat. Rev. Genet."},{"key":"2021050609175431700_bib17","doi-asserted-by":"publisher","first-page":"1531","DOI":"10.1534\/g3.113.006791","article-title":"Combinatorial effects of transposable elements on gene expression and phenotypic robustness in Drosophila melanogaster development.","volume":"3","author":"Clemmons","year":"2013","journal-title":"G3 (Bethesda)"},{"key":"2021050609175431700_bib18","doi-asserted-by":"publisher","first-page":"R21","DOI":"10.1186\/gb-2012-13-3-r21","article-title":"The generation of chromosomal deletions to provide extensive coverage and subdivision of the Drosophila melanogaster genome.","volume":"13","author":"Cook","year":"2012","journal-title":"Genome Biol."},{"key":"2021050609175431700_bib19","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1007\/s004270050079","article-title":"Developing compound eye in lozenge mutants of Drosophila: lozenge expression in the R7 equivalence group.","volume":"206","author":"Crew","year":"1997","journal-title":"Dev. Genes Evol."},{"key":"2021050609175431700_bib20","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1534\/genetics.109.107748","article-title":"A gain-of-function screen identifying genes required for growth and pattern formation of the Drosophila melanogaster wing.","volume":"183","author":"Cruz","year":"2009","journal-title":"Genetics"},{"key":"2021050609175431700_bib21","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1093\/genetics\/120.3.755","article-title":"A genetic analysis of the alpha-glycerophosphate oxidase locus in Drosophila melanogaster.","volume":"120","author":"Davis","year":"1988","journal-title":"Genetics"},{"key":"2021050609175431700_bib22","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1038\/ng1281","article-title":"Growth and specification of the eye are controlled independently by Eyegone and Eyeless in Drosophila melanogaster.","volume":"36","author":"Dominguez","year":"2004","journal-title":"Nat. Genet."},{"key":"2021050609175431700_bib23","doi-asserted-by":"publisher","first-page":"4721","DOI":"10.1242\/dev.02675","article-title":"JAK\/STAT signaling promotes regional specification by negatively regulating wingless expression in Drosophila.","volume":"133","author":"Ekas","year":"2006","journal-title":"Development"},{"key":"2021050609175431700_bib24","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1038\/nmeth.1356","article-title":"G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila.","volume":"6","author":"Evans","year":"2009","journal-title":"Nat. Methods"},{"key":"2021050609175431700_bib25","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/j.cell.2010.09.009","article-title":"Systematic protein location mapping reveals five principal chromatin types in Drosophila cells.","volume":"143","author":"Filion","year":"2010","journal-title":"Cell"},{"key":"2021050609175431700_bib26","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1007\/s00239-004-0312-4","article-title":"LTR retrotransposon-gene associations in Drosophila melanogaster.","volume":"62","author":"Ganko","year":"2006","journal-title":"J. Mol. Evol."},{"key":"2021050609175431700_bib27","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1093\/genetics\/131.1.99","article-title":"A genetic analysis of deltex and its interaction with the Notch locus in Drosophila melanogaster.","volume":"131","author":"Gorman","year":"1992","journal-title":"Genetics"},{"key":"2021050609175431700_bib28","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1038\/nature08816","article-title":"Transcriptional control of preadipocyte determination by Zfp423.","volume":"464","author":"Gupta","year":"2010","journal-title":"Nature"},{"key":"2021050609175431700_bib29","doi-asserted-by":"publisher","first-page":"R140","DOI":"10.1186\/gb-2013-14-12-r140","article-title":"Spatial expression of transcription factors in Drosophila embryonic organ development.","volume":"14","author":"Hammonds","year":"2013","journal-title":"Genome Biol."},{"key":"2021050609175431700_bib30","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/S0092-8674(00)81561-5","article-title":"OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and Olf signaling pathways.","volume":"100","author":"Hata","year":"2000","journal-title":"Cell"},{"key":"2021050609175431700_bib31","doi-asserted-by":"publisher","first-page":"1220","DOI":"10.1038\/sj.onc.1208243","article-title":"Evi3, a zinc-finger protein related to EBFAZ, regulates EBF activity in B-cell leukemia.","volume":"24","author":"Hentges","year":"2005","journal-title":"Oncogene"},{"key":"2021050609175431700_bib32","doi-asserted-by":"publisher","first-page":"1271","DOI":"10.1083\/jcb.201009107","article-title":"Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity.","volume":"191","author":"Hesse","year":"2010","journal-title":"J. Cell Biol."},{"key":"2021050609175431700_bib33","doi-asserted-by":"publisher","first-page":"2367","DOI":"10.1038\/sj.emboj.7600703","article-title":"FOG-1 recruits the NuRD repressor complex to mediate transcriptional repression by GATA-1.","volume":"24","author":"Hong","year":"2005","journal-title":"EMBO J."},{"key":"2021050609175431700_bib34","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1016\/j.ccr.2009.02.023","article-title":"ZNF423 is critically required for retinoic acid-induced differentiation and is a marker of neuroblastoma outcome.","volume":"15","author":"Huang","year":"2009","journal-title":"Cancer Cell"},{"key":"2021050609175431700_bib35","doi-asserted-by":"publisher","first-page":"2939","DOI":"10.1242\/dev.00522","article-title":"Two Pax genes, eye gone and eyeless, act cooperatively in promoting Drosophila eye development.","volume":"130","author":"Jang","year":"2003","journal-title":"Development"},{"key":"2021050609175431700_bib36","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1534\/genetics.112.140996","article-title":"Multiple barriers to nonhomologous DNA end joining during meiosis in Drosophila.","volume":"191","author":"Joyce","year":"2012","journal-title":"Genetics"},{"key":"2021050609175431700_bib37","doi-asserted-by":"publisher","first-page":"RESEARCH0084","DOI":"10.1186\/gb-2002-3-12-research0084","article-title":"The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective.","volume-title":"Genome Biol.","author":"Kaminker","year":"2002"},{"key":"2021050609175431700_bib38","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1038\/nature09726","article-title":"Intrinsic transition of embryonic stem-cell differentiation into neural progenitors.","volume":"470","author":"Kamiya","year":"2011","journal-title":"Nature"},{"key":"2021050609175431700_bib39","doi-asserted-by":"publisher","first-page":"2493","DOI":"10.1534\/genetics.107.080994","article-title":"Investigating the genetic circuitry of mastermind in Drosophila, a notch signal effector.","volume":"177","author":"Kankel","year":"2007","journal-title":"Genetics"},{"key":"2021050609175431700_bib40","doi-asserted-by":"publisher","first-page":"6569","DOI":"10.1073\/pnas.0732024100","article-title":"Molecular paleontology of transposable elements in the Drosophila melanogaster genome.","volume":"100","author":"Kapitonov","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021050609175431700_bib41","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1038\/nature09725","article-title":"Comprehensive analysis of the chromatin landscape in Drosophila melanogaster.","volume":"471","author":"Kharchenko","year":"2011","journal-title":"Nature"},{"key":"2021050609175431700_bib42","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1084\/jem.20121187","article-title":"Coordinated transcriptional regulation of bone homeostasis by Ebf1 and Zfp521 in both mesenchymal and hematopoietic lineages.","volume":"210","author":"Kiviranta","year":"2013","journal-title":"J. Exp. Med."},{"key":"2021050609175431700_bib43","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1534\/genetics.113.156737","article-title":"Highly improved gene targeting by germline-specific Cas9 expression in Drosophila.","volume":"195","author":"Kondo","year":"2013","journal-title":"Genetics"},{"key":"2021050609175431700_bib44","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1007\/s00427-007-0134-7","article-title":"DmOAZ, the unique Drosophila melanogaster OAZ homologue is involved in posterior spiracle development.","volume":"217","author":"Krattinger","year":"2007","journal-title":"Dev. Genes Evol."},{"key":"2021050609175431700_bib45","doi-asserted-by":"publisher","first-page":"5277","DOI":"10.1074\/jbc.M510004200","article-title":"OAZ regulates bone morphogenetic protein signaling through Smad6 activation.","volume":"281","author":"Ku","year":"2006","journal-title":"J. Biol. Chem."},{"key":"2021050609175431700_bib46","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/S1046-2023(03)00051-3","article-title":"Making a better RNAi vector for Drosophila: use of intron spacers.","volume":"30","author":"Lee","year":"2003","journal-title":"Methods"},{"key":"2021050609175431700_bib47","volume-title":"The Genome of Drosophila melanogaster.","author":"Lindsley","year":"1992"},{"key":"2021050609175431700_bib48","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030008","article-title":"Whole genome resequencing reveals natural target site preferences of transposable elements in Drosophila melanogaster.","volume":"7","author":"Linheiro","year":"2012","journal-title":"PLoS One"},{"key":"2021050609175431700_bib49","doi-asserted-by":"publisher","first-page":"55017","DOI":"10.1074\/jbc.M411240200","article-title":"The N termini of Friend of GATA (FOG) proteins define a novel transcriptional repression motif and a superfamily of transcriptional repressors.","volume":"279","author":"Lin","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2021050609175431700_bib50","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020002","article-title":"Retroviral elements and their hosts: insertional mutagenesis in the mouse germ line.","volume":"2","author":"Maksakova","year":"2006","journal-title":"PLoS Genet."},{"key":"2021050609175431700_bib51","doi-asserted-by":"publisher","first-page":"1765","DOI":"10.1016\/j.dnarep.2008.06.018","article-title":"Comparison of nonhomologous end joining and homologous recombination in human cells.","volume":"7","author":"Mao","year":"2008","journal-title":"DNA Repair (Amst.)"},{"key":"2021050609175431700_bib52","doi-asserted-by":"publisher","first-page":"30814","DOI":"10.1074\/jbc.M110.142869","article-title":"ZFP423 coordinates Notch and bone morphogenetic protein signaling, selectively up-regulating Hes5 gene expression.","volume":"285","author":"Masserdotti","year":"2010","journal-title":"J. Biol. Chem."},{"key":"2021050609175431700_bib53","doi-asserted-by":"publisher","first-page":"3480","DOI":"10.1074\/jbc.M805874200","article-title":"The role of zinc finger protein 521\/early hematopoietic zinc finger protein in erythroid cell differentiation.","volume":"284","author":"Matsubara","year":"2009","journal-title":"J. Biol. Chem."},{"key":"2021050609175431700_bib54","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1385\/1-59259-755-6:001","article-title":"Mobile genetic elements as natural tools for genome evolution.","volume":"260","author":"Miller","year":"2004","journal-title":"Methods Mol. Biol."},{"key":"2021050609175431700_bib55","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1038\/15490","article-title":"Epigenetic inheritance at the agouti locus in the mouse.","volume":"23","author":"Morgan","year":"1999","journal-title":"Nat. Genet."},{"key":"2021050609175431700_bib56","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1006\/dbio.2001.0229","article-title":"Dominant Drop mutants are gain-of-function alleles of the muscle segment homeobox gene (msh) whose overexpression leads to the arrest of eye development.","volume":"233","author":"Mozer","year":"2001","journal-title":"Dev. Biol."},{"key":"2021050609175431700_bib57","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000814","article-title":"A comprehensive map of insulator elements for the Drosophila genome.","volume":"6","author":"N\u00e8gre","year":"2010","journal-title":"PLoS Genet."},{"key":"2021050609175431700_bib58","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1038\/nature09990","article-title":"A cis-regulatory map of the Drosophila genome.","volume":"471","author":"N\u00e8gre","year":"2011","journal-title":"Nature"},{"key":"2021050609175431700_bib59","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1038\/nature15365","article-title":"Loss of Karma transposon methylation underlies the mantled somaclonal variant of oil palm.","volume":"525","author":"Ong-Abdullah","year":"2015","journal-title":"Nature"},{"key":"2021050609175431700_bib60","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.devcel.2011.10.029","article-title":"Tissue-specific coupling between insulin\/IGF and TORC1 signaling via PRAS40 in Drosophila.","volume":"22","author":"Pallares-Cartes","year":"2012","journal-title":"Dev. Cell"},{"key":"2021050609175431700_bib61","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1038\/ng1312","article-title":"Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome.","volume":"36","author":"Parks","year":"2004","journal-title":"Nat. Genet."},{"key":"2021050609175431700_bib62","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/S0091-679X(08)60927-9","article-title":"Imaging neuronal subsets and other cell types in whole-mount Drosophila embryos and larvae using antibody probes.","volume":"44","author":"Patel","year":"1994","journal-title":"Methods Cell Biol."},{"key":"2021050609175431700_bib63","doi-asserted-by":"publisher","first-page":"E2967","DOI":"10.1073\/pnas.1405500111","article-title":"Optimized CRISPR\/Cas tools for efficient germline and somatic genome engineering in Drosophila.","volume":"111","author":"Port","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021050609175431700_bib64","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.1534\/g3.115.019083","article-title":"Systematic evaluation of Drosophila CRISPR tools reveals safe and robust alternatives to autonomous gene drives in basic research.","volume":"5","author":"Port","year":"2015","journal-title":"G3 (Bethesda)"},{"key":"2021050609175431700_bib65","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010168","article-title":"Splinkerette PCR for mapping transposable elements in Drosophila.","volume":"5","author":"Potter","year":"2010","journal-title":"PLoS One"},{"key":"2021050609175431700_bib66","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1093\/genetics\/146.1.275","article-title":"Autosomal mutations affecting adhesion between wing surfaces in Drosophila melanogaster.","volume":"146","author":"Prout","year":"1997","journal-title":"Genetics"},{"key":"2021050609175431700_bib67","doi-asserted-by":"publisher","first-page":"1787","DOI":"10.1126\/science.1198374","article-title":"Identification of functional elements and regulatory circuits by Drosophila modENCODE.","volume":"330","author":"Roy","year":"2010","journal-title":"Science"},{"key":"2021050609175431700_bib68","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1534\/genetics.104.026658","article-title":"The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.","volume":"167","author":"Ryder","year":"2004","journal-title":"Genetics"},{"key":"2021050609175431700_bib69","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1534\/genetics.107.076216","article-title":"The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource.","volume":"177","author":"Ryder","year":"2007","journal-title":"Genetics"},{"key":"2021050609175431700_bib70","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1016\/j.molcel.2007.03.003","article-title":"PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase.","volume":"25","author":"Sancak","year":"2007","journal-title":"Mol. Cell"},{"key":"2021050609175431700_bib71","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1038\/ng1817","article-title":"Genome-wide analysis of Polycomb targets in Drosophila melanogaster.","volume":"38","author":"Schwartz","year":"2006","journal-title":"Nat. Genet."},{"key":"2021050609175431700_bib72","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1534\/genetics.105.044180","article-title":"Genetic interaction of Lobe with its modifiers in dorsoventral patterning and growth of the Drosophila eye.","volume":"171","author":"Singh","year":"2005","journal-title":"Genetics"},{"key":"2021050609175431700_bib73","doi-asserted-by":"publisher","first-page":"4771","DOI":"10.1242\/dev.02686","article-title":"Lobe and Serrate are required for cell survival during early eye development in Drosophila.","volume":"133","author":"Singh","year":"2006","journal-title":"Development"},{"key":"2021050609175431700_bib74","doi-asserted-by":"publisher","first-page":"51792","DOI":"10.3791\/51792","article-title":"Dissection and immunostaining of imaginal discs from Drosophila melanogaster.","volume":"91","author":"Spratford","year":"2014","journal-title":"J. Vis. Exp."},{"key":"2021050609175431700_bib75","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1038\/ng1314","article-title":"A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac.","volume":"36","author":"Thibault","year":"2004","journal-title":"Nat. Genet."},{"key":"2021050609175431700_bib76","doi-asserted-by":"publisher","first-page":"D759","DOI":"10.1093\/nar\/gky1003","article-title":"FlyBase 2.0: the next generation.","volume":"47","author":"Thurmond","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"2021050609175431700_bib77","doi-asserted-by":"publisher","first-page":"4159","DOI":"10.1523\/JNEUROSCI.17-11-04159.1997","article-title":"Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O\/E-1 to regulate gene expression: implications for olfactory neuronal development.","volume":"17","author":"Tsai","year":"1997","journal-title":"J. Neurosci."},{"key":"2021050609175431700_bib78","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1038\/ncb1547","article-title":"Insulin signalling to mTOR mediated by the Akt\/PKB substrate PRAS40.","volume":"9","author":"Vander Haar","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"2021050609175431700_bib79","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/j.ydbio.2008.06.038","article-title":"Temporal switching of regulation and function of eye gone (eyg) in Drosophila eye development.","volume":"321","author":"Wang","year":"2008","journal-title":"Dev. Biol."},{"key":"2021050609175431700_bib80","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1016\/j.mod.2009.08.005","article-title":"Reduction of Lobe leads to TORC1 hypoactivation that induces ectopic Jak\/STAT signaling to impair Drosophila eye development.","volume":"126","author":"Wang","year":"2009","journal-title":"Mech. Dev."},{"key":"2021050609175431700_bib81","doi-asserted-by":"publisher","first-page":"2602","DOI":"10.1038\/sj.emboj.7600725","article-title":"Eyg and Ey Pax proteins act by distinct transcriptional mechanisms in Drosophila development.","volume":"24","author":"Yao","year":"2005","journal-title":"EMBO J."},{"key":"2021050609175431700_bib82","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169931","article-title":"Non-homologous end joining and homology directed DNA repair frequency of double-stranded breaks introduced by genome editing reagents.","volume":"12","author":"Zaboikin","year":"2017","journal-title":"PLoS One"}],"container-title":["Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/genetics\/article-pdf\/216\/1\/117\/37821573\/genetics0117.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/genetics\/article-pdf\/216\/1\/117\/37821573\/genetics0117.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,7]],"date-time":"2021-05-07T04:19:00Z","timestamp":1620361140000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/216\/1\/117\/6065593"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,1]]},"references-count":82,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,9,1]]}},"URL":"https:\/\/doi.org\/10.1534\/genetics.120.303486","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,9,1]]},"published":{"date-parts":[[2020,9,1]]}}}