{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T10:48:30Z","timestamp":1789555710224,"version":"build-2803163510"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T00:00:00Z","timestamp":1078099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>One potential strategy for the control of malaria and other vector-borne diseases is the introduction into wild vector populations of genetic constructs that reduce vectorial capacity. An important caveat of this approach is that the genetic construct should have minimal fitness cost to the transformed vector. Previously, we produced transgenic Anopheles stephensi expressing either of two effector genes, a tetramer of the SM1 dodecapeptide or the phospholipase A2 gene (PLA2) from honeybee venom. Mosquitoes carrying either of these transgenes were impaired for Plasmodium berghei transmission. We have investigated the role of two effector genes for malaria parasite blockage in terms of the fitness imposed to the mosquito vector that expresses either molecule. By measuring mosquito survival, fecundity, fertility, and by running population cage experiments, we found that mosquitoes transformed with the SM1 construct showed no significant reduction in these fitness parameters relative to nontransgenic controls. The PLA2 transgenics, however, had reduced fitness that seemed to be independent of the insertion site of the transgene. We conclude that the fitness load imposed by refractory gene(s)-expressing mosquitoes depends on the effect of the transgenic protein produced in that mosquito. These results have important implications for implementation of malaria control via genetic modification of mosquitoes.<\/jats:p>","DOI":"10.1534\/genetics.166.3.1337","type":"journal-article","created":{"date-parts":[[2004,8,2]],"date-time":"2004-08-02T23:49:25Z","timestamp":1091490565000},"page":"1337-1341","source":"Crossref","is-referenced-by-count":92,"title":["Fitness of Anopheline Mosquitoes Expressing Transgenes That Inhibit Plasmodium Development"],"prefix":"10.1093","volume":"166","author":[{"given":"Luciano A","family":"Moreira","sequence":"first","affiliation":[{"name":"Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4955"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4955"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank H","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Biological Science, Galvin Life Sciences, Center for Tropical Disease Research and Training, University of Notre Dame, Notre Dame, Indiana 46556-0369"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Jacobs-Lorena","sequence":"additional","affiliation":[{"name":"Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4955"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,3,1]]},"reference":[{"key":"2022010522550062000_R1","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1603\/0022-2585-38.5.701","article-title":"Stable germ-line transformation of Culex quinquefasciatus (Diptera: Culicidae)","volume":"38","author":"Allen","year":"2001","journal-title":"J. Med. Entomol."},{"key":"2022010522550062000_R2","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1017\/S0031182099005570","article-title":"Plasmodium falciparum sporozoites increase feeding-associated mortality of their mosquito hosts Anopheles gambiae s.l.","volume":"120","author":"Anderson","year":"2000","journal-title":"Parasitology"},{"key":"2022010522550062000_R3","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1038\/35016096","article-title":"Stable germline transformation of the malaria mosquito Anopheles stephensi","volume":"405","author":"Catteruccia","year":"2000","journal-title":"Nature"},{"key":"2022010522550062000_R4","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1126\/science.1081453","article-title":"Impact of genetic manipulation on the fitness of Anopheles stephensi mosquitoes","volume":"299","author":"Catteruccia","year":"2003","journal-title":"Science"},{"key":"2022010522550062000_R5","doi-asserted-by":"crossref","first-page":"3748","DOI":"10.1073\/pnas.95.7.3748","article-title":"Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti","volume":"95","author":"Coates","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2022010522550062000_R6","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/S0965-1748(97)00093-3","article-title":"Rapid induction by a blood meal of a carboxypeptidase gene in the gut of the mosquito Anopheles gambiae","volume":"27","author":"Edwards","year":"1997","journal-title":"Insect Biochem. Mol. Biol."},{"key":"2022010522550062000_R7","volume-title":"Introduction to Quantitative Genetics","author":"Falconer","year":"1996"},{"key":"2022010522550062000_R8","doi-asserted-by":"crossref","first-page":"13278","DOI":"10.1073\/pnas.241491198","article-title":"Targeting Plasmodium ligands on mosquito salivary glands and midgut with a phage display peptide library","volume":"98","author":"Ghosh","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2022010522550062000_R9","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1046\/j.0962-1075.2001.00299.x","article-title":"Germline transformation of the malaria vector, Anopheles gambiae, with the piggyBac transposable element","volume":"10","author":"Grossman","year":"2001","journal-title":"Insect Mol. Biol."},{"key":"2022010522550062000_R10","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1017\/S0031182000077027","article-title":"Plasmodium yoelii nigeriensis: the effect of high and low intensity of infection upon the egg production and bloodmeal size of Anopheles stephensi during three gonotrophic cycles","volume":"111","author":"Hogg","year":"1995","journal-title":"Parasitology"},{"key":"2022010522550062000_R11","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/417452a","article-title":"Transgenic anopheline mosquitoes impaired in transmission of a malaria parasite","volume":"417","author":"Ito","year":"2002","journal-title":"Nature"},{"key":"2022010522550062000_R12","doi-asserted-by":"crossref","first-page":"3743","DOI":"10.1073\/pnas.95.7.3743","article-title":"Stable transformation of the yellow fever mosquito, Aedes aegypti, with the Hermes element from the housefly","volume":"95","author":"Jasinskiene","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2022010522550062000_R13","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1006\/bbrc.1999.0954","article-title":"Is green fluorescent protein toxic to the living cells?","volume":"260","author":"Liu","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2022010522550062000_R14","doi-asserted-by":"crossref","first-page":"10895","DOI":"10.1073\/pnas.97.20.10895","article-title":"Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes","volume":"97","author":"Moreira","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2022010522550062000_R15","doi-asserted-by":"crossref","first-page":"40839","DOI":"10.1074\/jbc.M206647200","article-title":"Bee venom phospholipase inhibits malaria parasite development in transgenic mosquitoes","volume":"277","author":"Moreira","year":"2002","journal-title":"J. Biol. Chem."}],"container-title":["Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/166\/3\/1337\/42059153\/genetics1337.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/166\/3\/1337\/42059153\/genetics1337.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T22:57:28Z","timestamp":1641423448000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/166\/3\/1337\/6050470"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,3,1]]},"references-count":15,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2004,3,1]]},"published-print":{"date-parts":[[2004,3,1]]}},"URL":"https:\/\/doi.org\/10.1534\/genetics.166.3.1337","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2004,3,1]]},"published":{"date-parts":[[2004,3,1]]}}}