{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T23:01:15Z","timestamp":1763766075109},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2014,10,3]],"date-time":"2014-10-03T00:00:00Z","timestamp":1412294400000},"content-version":"vor","delay-in-days":155,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Occupational Health"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec><jats:label \/><jats:p><jats:bold>Formation of the Nitrative DNA Lesion 8\u2010Nitroguanine is Associated with Asbestos Contents in Human Lung Tissues: A Pilot Study: Yusuke HIRAKU, <jats:italic>et al<\/jats:italic>. Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine\u2014<\/jats:bold><\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Asbestos causes lung cancer and malignant mesothelioma, and chronic inflammation is considered to participate in carcinogenesis. However, biomarkers to evaluate its carcinogenic risk have not been established. Reactive oxygen\/nitrogen species are generated in biological systems under inflammatory conditions and may contribute to carcinogenesis by causing DNA damage. In this study, we examined the relationship between the formation of 8nitroguanine (8\u2010nitroG), a mutagenic DNA lesion formed during inflammation, and asbestos contents in human lung tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We obtained non\u2010tumor lung tissues from patients with (n=15) and without mesothelioma (n=21). The expression of 8\u2010nitroG and related molecules was examined by immunohistochemistry, and their staining intensities were semiquantitatively evaluated. Asbestos contents in lung tissues were analyzed by analytical transmission electron microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In subjects without mesothelioma, staining intensities of 8\u2010nitroG and apurinic\/apyrimidinic endonuclease 1 (APE1) were significantly correlated with total asbestos and amphibole contents (<jats:italic>p<\/jats:italic>&lt;0.05), but not with chrysotile content. In mesothelioma patients, their staining intensities were not correlated with asbestos contents. The double immunofluorescence technique revealed that APE1 was expressed in 8\u2010nitroGpositive cells, suggesting that abasic sites were formed possibly due to the removal of 8\u2010nitroG. The staining intensities of 8\u2010oxo\u20107,8\u2010dihydro\u20102\u2019\u2010deoxyguanosine, an oxidative DNA lesion, and its repair enzyme 8\u2010oxoguanine DNA\u2010glycosylase were correlated with age (<jats:italic>p<\/jats:italic>&lt;0.05), but not with asbestos contents in subjects without mesothelioma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This is the first study to demonstrate that 8\u2010nitroG formation is associated with asbestos contents in human lung tissues. This finding raises a possibility that 8\u2010nitroG serves as a biomarker that can be used to evaluate asbestos exposure and carcinogenic risk.<\/jats:p><\/jats:sec>","DOI":"10.1539\/joh.13-0231-oa","type":"journal-article","created":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T00:45:09Z","timestamp":1393893909000},"page":"186-196","source":"Crossref","is-referenced-by-count":18,"title":["Formation of the Nitrative DNA Lesion 8\u2010Nitroguanine is Associated with Asbestos Contents in Human Lung Tissues: A Pilot Study"],"prefix":"10.1093","volume":"56","author":[{"given":"Yusuke","family":"Hiraku","sequence":"first","affiliation":[{"name":"Department of Environmental and Molecular Medicine Mie University Graduate School of Medicine Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoshi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Nagoya City Public Health Research Institute Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiji","family":"Shibata","sequence":"additional","affiliation":[{"name":"Department of Health and Psychosocial Medicine Aichi Medical University School of Medicine Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michihiro","family":"Kamijima","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health Nagoya City University Graduate School of Medical Sciences Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Hisanaga","sequence":"additional","affiliation":[{"name":"Center for Campus Health and Environment, Aichi University of Education Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Ma","sequence":"additional","affiliation":[{"name":"Faculty of Health Science Suzuka University of Medical Science Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shosuke","family":"Kawanishi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Suzuka University of Medical Science Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariko","family":"Murata","sequence":"additional","affiliation":[{"name":"Department of Environmental and Molecular Medicine Mie University Graduate School of Medicine Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,10,3]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"IARC.Asbestos (chrysotile amosite crocidolite tremolite actinolite and anthophyllite). 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