{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T23:00:06Z","timestamp":1771455606355,"version":"3.50.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2009,12,18]],"date-time":"2009-12-18T00:00:00Z","timestamp":1261094400000},"content-version":"vor","delay-in-days":47,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Occupational Health"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:bold>Broken Sperm, Cytoplasmic Droplets and Reduced Sperm Motility Are Principal Markers of Decreased Sperm Quality Due to Organophosphorus Pesticides in Rats: Ai O<jats:sc>kamura<\/jats:sc>, <jats:italic>et al<\/jats:italic>. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine<\/jats:bold><\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Recent reports have shown significant associations between organopshophorus pesticide (OP) exposure and decreased sperm motility in workers and laboratory animals. However, the notion that OPs possess spermatotoxicity has yet to be established. The aim of this study was to clarify the effects of OP exposure on detailed sperm toxicity markers, i.e., motility, morphology and sperm adenine nucleotide contents, and the histopathology of the testis and epididymis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ten\u2010week\u2010old Wistar rats were divided into 4 groups (n=10) and orally administered corn oil, dichlorvos (DDVP; 5, 10 mg\/kg) or diazinon (DZN; 3 mg\/kg) 6 days a week for 9 wk. Sperm motility and morphology markers were analyzed with a computer\u2010assisted sperm analysis (CASA) system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In addition to a significant decrease in acetylcholinesterase (AChE) activities and a significant increase in urinary OP metabolites, DDVP and DZN significantly reduced sperm motility, but they did not influence sperm adenine nucleotide contents. The OPs also significantly increased the percentage of broken sperm, and DDVP significantly increased the percentage of cytoplasmic droplets. Importantly, both OPs significantly increased cytoplasmic vacuolation and nuclear shrinkage in the epithelial cells of the ductus epididymis, whereas the testes did not show significant histopathological changes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The broken sperm and cytoplasmic droplets as well as reduced sperm motility were the relevant spermatotoxicity makers of DDVP and DZN. To our knowledge, this is the first report to suggest that the above\u2010mentioned OP\u2010induced spermatotoxicity is related to histopathological impairment of the caput epididymis.<\/jats:p><\/jats:sec>","DOI":"10.1539\/joh.l9019","type":"journal-article","created":{"date-parts":[[2009,12,21]],"date-time":"2009-12-21T06:05:08Z","timestamp":1261375508000},"page":"478-487","source":"Crossref","is-referenced-by-count":43,"title":["Broken Sperm, Cytoplasmic Droplets and Reduced Sperm Motility Are Principal Markers of Decreased Sperm Quality Due to Organophosphorus Pesticides in Rats"],"prefix":"10.1093","volume":"51","author":[{"given":"Ai","family":"Okamura","sequence":"first","affiliation":[{"name":"Department of Occupational and Environmental Health Nagoya University Graduate School of Medicine Japan"},{"name":"Research Fellow of the Japan Society for the Promotion of Science Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michihiro","family":"Kamijima","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health Nagoya University Graduate School of Medicine Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Ohtani","sequence":"additional","affiliation":[{"name":"Department of Hazard Evaluation and Epidemiology Research Group National Institute of Occupational Safety and Health Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Yamanoshita","sequence":"additional","affiliation":[{"name":"Unit of Environmental Health Sciences, Department of Biomedical Sciences College of Life and Health Sciences, Chubu University Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daichi","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health Nagoya University Graduate School of Medicine Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health Nagoya University Graduate School of Medicine Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maiko","family":"Miyata","sequence":"additional","affiliation":[{"name":"Unit of Environmental Health Sciences, Department of Biomedical Sciences College of Life and Health Sciences, Chubu University Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Ueyama","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Nagoya University School of Health Sciences Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayoshi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Nagoya University School of Health Sciences Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryota","family":"Imai","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Nagoya University School of Health Sciences Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Takagi","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Nagoya University School of Health Sciences Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamie","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health Nagoya University Graduate School of Medicine Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,12,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"251","article-title":"Basic toxicology of pesticides","volume":"12","author":"Costa LG","year":"1997","journal-title":"Occup Med"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.5271\/sjweh.573"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2003.11.023"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2006.08.005"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0274(199908)36:2<230::AID-AJIM2>3.0.CO;2-6"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.011091237"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh444"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1539\/joh.46.109"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1179\/oeh.2006.12.4.355"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/reprod\/120.2.385"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2005.05.015"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2006.05.001"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-6238(00)00094-0"},{"key":"e_1_2_1_15_1","first-page":"230","article-title":"Influence of diazinon and deltamethrin on reproductive organs and fertility of male rats","volume":"101","author":"Abd el\u2010Aziz MI","year":"1994","journal-title":"Dtsch Tierarztl Wochenschr"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.06.028"},{"key":"e_1_2_1_17_1","first-page":"357","article-title":"How do sperm swim? Molecular mechanisms underlying sperm motility","volume":"49","author":"Luconi M","year":"2003","journal-title":"Cell Mol Biol (Noisy\u2010le\u2010grand)"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.033548"},{"key":"e_1_2_1_19_1","volume-title":"Interim Reregistration Eligibility Decision for Dichlorvos (DDVP)","author":"Environmental Protection Agency (EPA)","year":"2006"},{"key":"e_1_2_1_20_1","volume-title":"Interim Reregistration Eligibility Decision: Diazinon","author":"Environmental Protection Agency (EPA)","year":"2004"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(70)90082-7"},{"key":"e_1_2_1_22_1","first-page":"277","article-title":"Presence and localization of molecules related to the cholinergic system in developing rat testis","volume":"43","author":"Palmero S","year":"1999","journal-title":"Eur J Histochem"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.29.217"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20303"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520297009067484"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1532-0456(02)00019-4"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.12.030"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.11.4425"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD05120"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmi053"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh555"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.2.612"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(00)00700-7"},{"key":"e_1_2_1_34_1","first-page":"109","article-title":"Maturation of redox regulatory mechanisms in the epididymis","volume":"53","author":"Aitken RJ","year":"1998","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.03.022"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(96)01470-4"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19970605"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijheh.2006.08.006"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00420-006-0128-1"}],"container-title":["Journal of Occupational Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1539%2Fjoh.L9019","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1539\/joh.L9019","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1539\/joh.L9019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T18:18:19Z","timestamp":1705083499000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/joh\/article\/51\/6\/478-487\/7270140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["10.1539\/joh.L9019"],"URL":"https:\/\/doi.org\/10.1539\/joh.l9019","archive":["Portico"],"relation":{},"ISSN":["1341-9145","1348-9585"],"issn-type":[{"value":"1341-9145","type":"print"},{"value":"1348-9585","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,11]]}}}