{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T20:01:21Z","timestamp":1772654481014,"version":"3.50.1"},"reference-count":0,"publisher":"Romanian Society of Gastroenterology and Hepatology","issue":"1","license":[{"start":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T00:00:00Z","timestamp":1680220800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JGLD"],"abstract":"<jats:p>Background and Aims: MicroRNAs (miR) have altered expression in multiple autoimmune disorders including inflammatory bowel disease. The aim of the study was to assess the tissue and circulating miR-31, miR-200b, and miR-200c expression levels as potential biomarkers for intestinal disease activity in patients with Crohn\u2019s disease (CD).\r\nMethods: The study included 45 patients with histopathological confirmed CD and active disease (defined as fecal calprotectin &gt;50 \u03bcg\/g and Simple Endoscopic Score (SES) of CD &gt;3), and 21 subjects as controls for the validation cohort. Demographic and clinical data, biomarkers (fecal calprotectin), endoscopy data, the expression levels of miR-31, miR-200b, and miR-200c in tissue and serum were assessed (by RT-PCR). Receiver operating characteristic analysis was performed to assess the miR-31, miR-200b, and miR-200c expression levels as potential biomarkers for active CD.\r\nResults: Mean fecal calprotectin was 1540\u00b1890 \u03bcg\/g. Mean SES-CD was 8.9\u00b14.2. Tissue and circulating miR- 31 were significantly correlated with fecal calprotectin (r=0.81, r=0.83, p&lt;0.01) and with SES-CD (r=0.82, r=0.79, p&lt;0.01). The expression level of miR-31 was significantly upregulated in CD tissue cases compared to the control tissue samples (6.24\u00b11.57 vs. 3.70\u00b11.44; p &lt;0.01). Similarly, serum miR-31 expression levels in CD patients were significantly upregulated compared to the control serum samples (0.78\u00b10.42 vs. -2.07\u00b11.00; p&lt;0.01). The expression levels of tissue miR-200b and miR-200c were significantly upregulated in CD tissue cases compared to the control tissue samples (-5.25\u00b10.93 vs. -4.69\u00b10.80, p=0.03 for miR-200b, and -0.86\u00b10.96 vs. 0.39\u00b10.66, p&lt;0.01 for miR-200c). Similarly, serum miR-200b and miR-200c expression levels in CD patients were significantly upregulated compared to the control serum samples (p &lt; 0.05). Receiver operating characteristic analysis revealed that the expression levels of the selected miRNAs could help to discriminate active CD patients from healthy controls with very good specificity and sensitivity.\r\nConclusions: Tissue and circulating miR-31, miR-200b, and miR-200c reflect disease activity in CD patients and can be used as biomarkers for active disease.<\/jats:p>","DOI":"10.15403\/jgld-4656","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T18:24:08Z","timestamp":1680287048000},"page":"30-38","source":"Crossref","is-referenced-by-count":12,"title":["Tissue and Circulating MicroRNA-31, MicroRNA-200b, and MicroRNA-200c Reflects Disease Activity in Crohn\u2018s Disease Patients: Results from the BIOMIR Study"],"prefix":"10.15403","volume":"32","author":[{"given":"Cristina","family":"Tocia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrei","family":"Dumitru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bogdan","family":"Mateescu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucian","family":"Negreanu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"State","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgeta Camelia","family":"Cozaru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anca Florentina","family":"Mitroi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Costel","family":"Brinzan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Razvan","family":"Popescu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicoleta","family":"Leopa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miorita Melina","family":"Iordache","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihaela","family":"Manea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Matei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugen","family":"Dumitru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luana","family":"Alexandrescu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"6121","published-online":{"date-parts":[[2023,3,31]]},"container-title":["Journal of Gastrointestinal and Liver Diseases"],"original-title":[],"link":[{"URL":"https:\/\/jgld.ro\/jgld\/index.php\/jgld\/article\/download\/4656\/1910","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/jgld.ro\/jgld\/index.php\/jgld\/article\/download\/4656\/1910","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T21:14:12Z","timestamp":1759785252000},"score":1,"resource":{"primary":{"URL":"https:\/\/jgld.ro\/jgld\/index.php\/jgld\/article\/view\/4656"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,31]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,4,1]]}},"URL":"https:\/\/doi.org\/10.15403\/jgld-4656","relation":{},"ISSN":["1842-1121","1841-8724"],"issn-type":[{"value":"1842-1121","type":"electronic"},{"value":"1841-8724","type":"print"}],"subject":[],"published":{"date-parts":[[2023,3,31]]}}}