{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:13:57Z","timestamp":1758845637097,"version":"3.40.5"},"reference-count":25,"publisher":"American Academy of Pediatrics (AAP)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,12,1]]},"abstract":"<jats:sec>\n                  <jats:title>Objective.<\/jats:title>\n                  <jats:p>To investigate the effect of inhaled nitric oxide (NO) treatment in newborns with persistent pulmonary hypertension on adenosine 5\u2032-diphosphate (ADP)-dependent platelet activation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods.<\/jats:title>\n                  <jats:p>After parental informed consent, infants with persistent pulmonary hypertension of the newborn were randomly assigned to receive conventional treatment (control group) or treatment with 40 parts per million of inhaled NO. Platelet activation was measured at time of entry and 30 minutes later by surface expression of P-selectin in response to increasing concentrations of the agonist ADP (0, 2, 5, 10, and 20 \u03bcM) using fluorescence-activated flow cytometry.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results.<\/jats:title>\n                  <jats:p>We examined 11 infants in the inhaled NO group and 13 in the control group. P-selectin expression, quantified as mean fluorescence, was not significantly different in the two groups of patients at baseline. Median percent change from baseline fluorescence was assessed using the Wilcoxon matched-pairs signed-rank test. At 30 minutes after enrollment there were no statistically significant changes from baseline fluorescence in either group of patients and at all ADP concentrations.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion.<\/jats:title>\n                  <jats:p>Thirty minutes of exposure to 40 ppm of inhaled NO does not inhibit ADP-dependent platelet activation as measured by surface expression of P-selectin in infants with persistent pulmonary hypertension of the newborn.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1542\/peds.102.6.1390","type":"journal-article","created":{"date-parts":[[2004,8,13]],"date-time":"2004-08-13T23:44:32Z","timestamp":1092440672000},"page":"1390-1393","source":"Crossref","is-referenced-by-count":10,"title":["Inhaled Nitric Oxide Does Not Affect Adenosine 5\u2032-Diphosphate-dependent Platelet Activation in Infants With Persistent Pulmonary Hypertension of the Newborn"],"prefix":"10.1542","volume":"102","author":[{"given":"Helen","family":"Christou","sequence":"first","affiliation":[{"name":"From the Divisions of Newborn Medicine and"},{"name":"Developmental and Newborn Biology, the Departments of"},{"name":"Newborn Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbarajean","family":"Magnani","sequence":"additional","affiliation":[{"name":"Laboratory Medicine and Pathology,"},{"name":"Anesthesia, Brigham and Women's Hospital, Harvard Medical School; and the"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Morse","sequence":"additional","affiliation":[{"name":"Anesthesia, Brigham and Women's Hospital, Harvard Medical School; and the"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth N.","family":"Allred","sequence":"additional","affiliation":[{"name":"Neurology, Children's Hospital; the Departments of"},{"name":"Department of Biostatistics, Harvard School of Public Health, Boston, Massachusetts."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda J.","family":"Van Marter","sequence":"additional","affiliation":[{"name":"From the Divisions of Newborn Medicine and"},{"name":"Developmental and Newborn Biology, the Departments of"},{"name":"Newborn Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Wessel","sequence":"additional","affiliation":[{"name":"Anesthesia,"},{"name":"Cardiology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stella","family":"Kourembanas","sequence":"additional","affiliation":[{"name":"From the Divisions of Newborn Medicine and"},{"name":"Developmental and Newborn Biology, the Departments of"},{"name":"Newborn Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"417","published-online":{"date-parts":[[1998,12,1]]},"reference":[{"key":"2021121000343349100_B1","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/0140-6736(92)92687-B","article-title":"Low-dose inhalation nitric oxide in persistent pulmonary hypertension of the newborn.","volume":"340","author":"Kinsella","year":"1992","journal-title":"Lancet."},{"key":"2021121000343349100_B2","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1016\/0140-6736(92)92686-A","article-title":"Inhaled nitric oxide in persistent pulmonary hypertension of the newborn.","volume":"340","author":"Roberts","year":"1992","journal-title":"Lancet."},{"key":"2021121000343349100_B3","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/S0022-3476(97)70245-2","article-title":"Effect of inhaled nitric oxide on endothelin-1 and cyclic guanosine 5\u2032-monophosphate plasma concentrations in newborn infants with persistent pulmonary hypertension.","volume":"130","author":"Christou","year":"1997","journal-title":"J Pediatr."},{"key":"2021121000343349100_B4","doi-asserted-by":"crossref","first-page":"2128","DOI":"10.1161\/01.CIR.88.5.2128","article-title":"Use of inhaled nitric oxide and acetylcholine in the evaluation of pulmonary hypertension and endothelial function after cardiopulmonary bypass.","volume":"88","author":"Wessel","year":"1993","journal-title":"Circulation."},{"key":"2021121000343349100_B5","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/S0022-3476(05)83175-0","article-title":"Acute effects of inhaled nitric oxide in children with severe hypoxemic respiratory failure.","volume":"124","author":"Abman","year":"1994","journal-title":"J Pediatr."},{"key":"2021121000343349100_B6","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1097\/00000542-199507000-00007","article-title":"Inhibition of platelet aggregation by inhaled nitric oxide in patients with acute respiratory distress syndrome.","volume":"83","author":"Samama","year":"1995","journal-title":"Anesthesiology."},{"key":"2021121000343349100_B7","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1056\/NEJM199702273360902","article-title":"Inhaled nitric oxide and persistent pulmonary hypertension of the newborn.","volume":"336","author":"Roberts","year":"1997","journal-title":"N Engl J Med."},{"key":"2021121000343349100_B8","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0022-3476(97)70124-0","article-title":"Randomized, multicenter trial of inhaled nitric oxide and high-frequency oscillatory ventilation in severe, persistent pulmonary hypertension of the newborn.","volume":"131","author":"Kinsella","year":"1997","journal-title":"J Pediatr."},{"key":"2021121000343349100_B9","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1056\/NEJM199702273360901","article-title":"Inhaled nitric oxide in full-term and nearly full-term infants with hypoxic respiratory failure.","volume":"336","author":"The Neonatal Inhaled Nitric Oxide Study Group","year":"1997","journal-title":"N Engl J Med."},{"key":"2021121000343349100_B10"},{"key":"2021121000343349100_B11","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1542\/peds.92.4.606","article-title":"Inhaled nitric oxide in the management of a premature infant with severe respiratory distress and pulmonary hypertension.","volume":"92","author":"Abman","year":"1993","journal-title":"Pediatrics."},{"key":"2021121000343349100_B12","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1097\/00005344-199303000-00006","article-title":"Antiplatelet effects of nitroglycerin in healthy subjects and in patients with stable angina pectoris.","volume":"21","author":"Chirkov","year":"1993","journal-title":"J Cardiovasc Pharmacol."},{"key":"2021121000343349100_B13","first-page":"161","article-title":"Nitrovasodilators and cGMP inhibit human platelet activation.","volume":"2","author":"Doni","year":"1991","journal-title":"Cardioscience."},{"key":"2021121000343349100_B14","doi-asserted-by":"crossref","first-page":"5193","DOI":"10.1073\/pnas.87.13.5193","article-title":"An L-arginine\/nitric oxide pathway present in human platelets regulates aggregation.","volume":"87","author":"Radomski","year":"1990","journal-title":"Proc Natl Acad Sci USA."},{"key":"2021121000343349100_B15"},{"key":"2021121000343349100_B16","first-page":"A39","article-title":"Inhaled nitric oxide does not inhibit platelets in healthy human volunteers.","volume":"85","author":"Sherman","year":"1996","journal-title":"Anesthesiology."},{"key":"2021121000343349100_B17","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1042\/cs0910225","article-title":"Effects of inhaled nitric oxide compared with aspirin on platelet function in vivo in healthy subjects.","volume":"91","author":"Albert","year":"1996","journal-title":"Clin Sci."},{"key":"2021121000343349100_B18","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1002\/cyto.990100413","article-title":"Correlated measurement of platelet release and aggregation in whole blood.","volume":"10","author":"Ault","year":"1989","journal-title":"Cytometry."},{"key":"2021121000343349100_B19","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1083\/jcb.101.3.880","article-title":"A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation.","volume":"101","author":"Stenberg","year":"1985","journal-title":"J Cell Biol."},{"key":"2021121000343349100_B20","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/S0146-0005(97)80022-5","article-title":"The relevance of developmental hemostasis to hemorrhagic disorders of newborns.","volume":"21","author":"Andrew","year":"1997","journal-title":"Sem Perinatol."},{"key":"2021121000343349100_B21","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/S0022-3476(82)80766-X","article-title":"Platelet dysfunction and coagulopathy in intraventricular hemorrhage in the premature infant.","volume":"100","author":"Setzer","year":"1982","journal-title":"J Pediatr."},{"key":"2021121000343349100_B22","first-page":"H1640","article-title":"Effects of nitric oxide\/EDRF on platelet surface glycoproteins.","volume":"270","author":"Michelson","year":"1996","journal-title":"Am J Physiol."},{"key":"2021121000343349100_B23","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1097\/00000542-199702000-00013","article-title":"Inhaled nitric oxide inhibits platelet aggregation after pulmonary embolism in pigs.","volume":"86","author":"Gries","year":"1997","journal-title":"Anesthesiology."},{"key":"2021121000343349100_B24","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1016\/0140-6736(93)90802-N","article-title":"Bleeding time prolongation and NO inhalation.","volume":"341","author":"Hogman","year":"1993","journal-title":"Lancet."},{"key":"2021121000343349100_B25","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1161\/01.CIR.94.8.1919","article-title":"Inhaled nitric oxide increases coronary artery patency after thrombolysis.","volume":"94","author":"Adrie","year":"1996","journal-title":"Circulation."}],"container-title":["Pediatrics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/publications.aap.org\/pediatrics\/article-pdf\/102\/6\/1390\/890197\/1390.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/publications.aap.org\/pediatrics\/article-pdf\/102\/6\/1390\/890197\/1390.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T07:35:29Z","timestamp":1639121729000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.aap.org\/pediatrics\/article\/102\/6\/1390\/62139\/Inhaled-Nitric-Oxide-Does-Not-Affect-Adenosine-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,12,1]]},"references-count":25,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1998,12,1]]},"published-print":{"date-parts":[[1998,12,1]]}},"URL":"https:\/\/doi.org\/10.1542\/peds.102.6.1390","relation":{},"ISSN":["1098-4275","0031-4005"],"issn-type":[{"type":"electronic","value":"1098-4275"},{"type":"print","value":"0031-4005"}],"subject":[],"published":{"date-parts":[[1998,12,1]]}}}