{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:13:39Z","timestamp":1791094419421,"version":"4.1.0"},"reference-count":37,"publisher":"American Academy of Pediatrics (AAP)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,7,1]]},"abstract":"<jats:p>BACKGROUND. Group B streptococcal (GBS) infection remains a leading cause of neonatal sepsis. Currently, the management guidelines of neonates born to women with unknown GBS status at delivery are unclear. In this cohort, who undergo at least a 48-hour observation, a rapid method of detection of GBS colonization would allow targeted evaluation and treatment, as well as prevent delayed discharge.<\/jats:p>\n               <jats:p>OBJECTIVE. The goal of this research was to evaluate the validity of rapid fluorescent real-time polymerase chain reaction in comparison with standard culture to detect GBS colonization in infants born to women whose GBS status is unknown at delivery.<\/jats:p>\n               <jats:p>DESIGN\/METHODS. Neonates at &amp;gt;32 weeks' gestation born to women whose GBS status was unknown at delivery were included. Samples were obtained from the ear, nose, rectum, and gastric aspirate for immediate culture and real-time polymerase chain reaction after DNA extraction using the LightCycler. Melting point curves were generated, and confirmatory agar gel electrophoresis was performed.<\/jats:p>\n               <jats:p>RESULTS. The study population (n = 94) had a mean \u00b1 SD gestational age of 38 \u00b1 2 weeks and birth weight of 3002 \u00b1 548 g. The rates of GBS colonization by culture were 17% and 51% by real-time polymerase chain reaction. The 4 surface sites had comparable rates of GBS. The overall sensitivities, specificities, and positive and negative predictive values of real-time polymerase chain reaction were: 90%, 80.3%, 28%, and 98.9%.<\/jats:p>\n               <jats:p>CONCLUSIONS. Real-time polymerase chain reaction resulted in a threefold higher rate of detection of GBS colonization and had an excellent negative predictive value in a cohort of neonates with unknown maternal GBS status at delivery. Thus, real-time polymerase chain reaction would be a useful clinical tool in the management of those infants potentially at risk for invasive GBS infection and would allow earlier discharge for those found to be not at risk.<\/jats:p>","DOI":"10.1542\/peds.2005-1594","type":"journal-article","created":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T16:42:48Z","timestamp":1152204168000},"page":"14-22","source":"Crossref","is-referenced-by-count":29,"title":["Real-Time Polymerase Chain Reaction for the Rapid Detection of Group B Streptococcal Colonization in Neonates"],"prefix":"10.1542","volume":"118","author":[{"given":"Girija","family":"Natarajan","sequence":"first","affiliation":[{"name":"Division of Neonatology, Children's Hospital of Michigan, Detroit, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvette R.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Children's Hospital of Michigan, Detroit, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Cancer Genetics Research, Henry Ford Health Systems and Josephine Ford Cancer Center, Detroit, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang Mei","family":"Chen","sequence":"additional","affiliation":[{"name":"Cancer Genetics Research, Henry Ford Health Systems and Josephine Ford Cancer Center, Detroit, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"Worsham","sequence":"additional","affiliation":[{"name":"Cancer Genetics Research, Henry Ford Health Systems and Josephine Ford Cancer Center, Detroit, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"417","published-online":{"date-parts":[[2006,7,1]]},"reference":[{"key":"2021120823103048500_R1","doi-asserted-by":"crossref","unstructured":"Schrag SJ, Zywicki S, Farley MM, et al. 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