{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:08:43Z","timestamp":1761764923030,"version":"3.40.5"},"reference-count":28,"publisher":"American Academy of Pediatrics (AAP)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,7,1]]},"abstract":"<jats:p>OBJECTIVE. The objective of this study was to identify a specific fingerprint chromatogram model of serum proteins for early screening and diagnosis of Hirschsprung disease.<\/jats:p>\n               <jats:p>METHODS. To detect the protein mass spectrograms of 78 serum specimens (42 specimens of Hirschsprung disease, 16 specimens of adhesive ileus including appendicitis and Meckel diverticulum after operation and inflammatory bowel disease, and 20 specimens of normal control subjects), we used surface-enhanced laser desorption\/ionization time of flight mass spectrometry technology, combined with bioinformatics methods (support vector machine) to develop and compare protein mass spectrograms from serum samples.<\/jats:p>\n               <jats:p>RESULTS. We identified 3 protein markers, the mass-to-charge ratio of which is positioned at 3221.7, 5639.2, and 6884.2 from the fingerprint chromatogram model of serum protein for early screening and diagnosis of Hirschsprung disease. The markers had 100% sensitivity and specificity.<\/jats:p>\n               <jats:p>CONCLUSION. The fingerprint chromatogram model of serum protein using surface-enhanced laser desorption\/ionization time of flight mass spectrometry technology combining support vector machine is a new method of early screening and diagnosis of Hirschsprung disease that is worthy of additional research and application.<\/jats:p>","DOI":"10.1542\/peds.2006-1364","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T00:54:47Z","timestamp":1181004887000},"page":"e56-e60","source":"Crossref","is-referenced-by-count":7,"title":["Detection and Significance of Serum Protein Marker of Hirschsprung Disease"],"prefix":"10.1542","volume":"120","author":[{"given":"Jia-xiang","family":"Wang","sequence":"first","affiliation":[{"name":"Pediatric Surgery Department, First Affiliated Hospital, Zhengzhou University, Zhengzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan","family":"Qin","sequence":"additional","affiliation":[{"name":"Pediatric Surgery Department, First Affiliated Hospital, Zhengzhou University, Zhengzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiu-liang","family":"Liu","sequence":"additional","affiliation":[{"name":"Pediatric Surgery Department, First Affiliated Hospital, Zhengzhou University, Zhengzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He-ying","family":"Yang","sequence":"additional","affiliation":[{"name":"Pediatric Surgery Department, First Affiliated Hospital, Zhengzhou University, Zhengzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-zhong","family":"Fan","sequence":"additional","affiliation":[{"name":"Pediatric Surgery Department, First Affiliated Hospital, Zhengzhou University, Zhengzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie-kai","family":"Yu","sequence":"additional","affiliation":[{"name":"Cancer Institute, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Zheng","sequence":"additional","affiliation":[{"name":"Cancer Institute, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"417","published-online":{"date-parts":[[2007,7,1]]},"reference":[{"key":"2021120901584071700_R1","doi-asserted-by":"crossref","unstructured":"Zhang L, Yu W, He T, et al. Contribution of human alpha-defensin 1, 2, and 3 to the anti-HIV-1 activity of CD8 antiviral factor. Science.\u20082002;298:995\u20131000","DOI":"10.1126\/science.1076185"},{"key":"2021120901584071700_R2","doi-asserted-by":"crossref","unstructured":"Spencer D, Robson L, Purdy D, et al. A strategy for mapping and neutralizing conformational immunogenic sites on protein therapeutics. Proteomics.\u20082002;2:271\u2013279","DOI":"10.1002\/1615-9861(200203)2:3<271::AID-PROT271>3.0.CO;2-W"},{"key":"2021120901584071700_R3","doi-asserted-by":"crossref","unstructured":"Liu Y. Active learning with support vector machine applied to gene expression data for cancer classification. J Chem Inf Comput Sci.\u20082004;44:1936\u20131941","DOI":"10.1021\/ci049810a"},{"key":"2021120901584071700_R4","doi-asserted-by":"crossref","unstructured":"Parisi MA, Kapur RP. Genetics of Hirschsprung disease. Curr Opin Pediatr.\u20082000;12:610\u2013617","DOI":"10.1097\/00008480-200012000-00017"},{"key":"2021120901584071700_R5","unstructured":"Raffenserger JG. Swenson's Pediatric Surgery. Chicago, IL: University of Chicago Press; 1980:507\u2013531"},{"key":"2021120901584071700_R6","doi-asserted-by":"crossref","unstructured":"De Brito IA, Maksoud JG. Evolution with age of the acetylcholinesterase activity in rectal suction biopsy in Hirschsprung disease. J Pediatr Surg.\u20081987;22:425\u2013430","DOI":"10.1016\/S0022-3468(87)80262-2"},{"key":"2021120901584071700_R7","doi-asserted-by":"crossref","unstructured":"Morais MB, Sdepanian VL, Tahan S, et al. Effectiveness of anorectal manometry using the balloon method to identify the inhibitory recto-anal reflex for diagnosis of Hirschsprung's disease [in Portuguese]. Rev Assoc Med Bras.\u20082005;51:313\u2013317","DOI":"10.1590\/S0104-42302005000600013"},{"key":"2021120901584071700_R8","unstructured":"Zhou XL, Chen FB, Ou BY. Clinical significance of anorectal manometry in newborn in Hirschsprung disease. Chin J Pediatr.\u20082004;9:681\u2013683"},{"key":"2021120901584071700_R9","unstructured":"Childs B, Beaudet AL, Valle D, Kinzler KW, Vogelstein B. Hirschsprung disease. In: Scriver CR, Sly WS, eds. The Metabolic and Molecular Bases of Inherited Disease. New York, NY: The McGraw-Hill Companies; 2004:1\u201347"},{"key":"2021120901584071700_R10","doi-asserted-by":"crossref","unstructured":"Amiel J, Laudier B, Attie-Bitach T, et al. Polyalanine expansion and frameshift mutations of the paired-like homeobox gene PHOX2B in congenital central hypoventilation syndrome. Nat Genet.\u20082003;33:459\u2013461","DOI":"10.1038\/ng1130"},{"key":"2021120901584071700_R11","doi-asserted-by":"crossref","unstructured":"Takahashi M, Ritz J, Cooper GM. Activation of a novel human transforming gene, ret, by DNA rearrangement. Cell.\u20081985;42:281\u2013288","DOI":"10.1016\/0092-8674(85)90115-1"},{"key":"2021120901584071700_R12","doi-asserted-by":"crossref","unstructured":"Kapur RP. Hirschsprung disease and other enteric dysganglionoses. Crit Rev Clin Lab Sci.\u20081999;36:225\u2013273","DOI":"10.1080\/10408369991239204"},{"key":"2021120901584071700_R13","doi-asserted-by":"crossref","unstructured":"Bordeaux MC, Forcet C, Granger L, et al. The RET proto-oncogene induces apoptosis: a novel mechanism for Hirschsprung's disease. EMBO J.\u20082000;19:4056\u20134063","DOI":"10.1093\/emboj\/19.15.4056"},{"key":"2021120901584071700_R14","unstructured":"Warnovara K, Salo M, Sainio K, et al. Distribution of glial cell line-derived neurotrophic factor mRNA in human colon suggests roles for muscularis mucosae in innervation. Pediatr Surg Int.\u20081998;13:581\u2013583"},{"key":"2021120901584071700_R15","doi-asserted-by":"crossref","unstructured":"Bar KJ, Facer P, Williams NS, Tam PK, Anand P. Glial-derived neurotrophic factor in human adult and fetal intestine and in Hirschsprung's disease. Gastroenterology.\u20081997;112:1381\u20131385","DOI":"10.1016\/S0016-5085(97)70154-9"},{"key":"2021120901584071700_R16","unstructured":"Yang HY, Liu QL, Wang JX, Xu HF. Clinical study of multiple zonal aganglionosis in long segment Hirschsprung disease. Natl Med J China.\u20082005;39:2772\u20132774"},{"key":"2021120901584071700_R17","doi-asserted-by":"crossref","unstructured":"Weinberger SR, Boschetti E, Santambien P, Brenac V. Surface-enhanced laser desorption-ionization retentate chromatography mass spectrometry (SELDI-RC-MS): a new method for rapid development of process chromatography conditions. J Chromatogr B Analyt Technol Biomed Life Sci.\u20082002;782:307\u2013316","DOI":"10.1016\/S1570-0232(02)00564-0"},{"key":"2021120901584071700_R18","doi-asserted-by":"crossref","unstructured":"Vlahou A, Schorge JO, Gregory BW, Coleman RL. Diagnosis of ovarian cancer using decision tree classification of mass spectral data. Biomed Biotechnol.\u20082003;2003:308\u2013314","DOI":"10.1155\/S1110724303210032"},{"key":"2021120901584071700_R19","doi-asserted-by":"crossref","unstructured":"Wagner M, Naik DN, Pothen A, et al. Computational protein biomarker prediction: a case study for prostate cancer. BMC Bioinformatics.\u20082004;5:26A","DOI":"10.1186\/1471-2105-5-26"},{"key":"2021120901584071700_R20","doi-asserted-by":"crossref","unstructured":"Vlahou A, Laronga C, Wilson L, et al. A novel approach toward development of a rapid blood test for breast cancer. Clin Breast Cancer.\u20082003;4:203\u2013209","DOI":"10.1016\/S1526-8209(11)70627-9"},{"key":"2021120901584071700_R21","unstructured":"Xiao XY, Tang Y, Wei XP, He DC. A preliminary analysis of non-small cell lung cancer biomarkers in serum. Biomed Environ Sci.\u20082003;16:140\u2013147"},{"key":"2021120901584071700_R22","unstructured":"Wang JX, Zhang B, Yu JK, et al. Using ANN and serum protein pattern models in liver cancer diagnosis [in Chinese]. Zhonghua Yi Xue Za Zhi.\u20082005;85:189\u2013192"},{"key":"2021120901584071700_R23","doi-asserted-by":"crossref","unstructured":"Chen YD, Zheng S, Yu JK, Hu X. Clin Cancer Res.\u20082004;10:8380\u20138385","DOI":"10.1158\/1078-0432.CCR-1162-03"},{"key":"2021120901584071700_R24","doi-asserted-by":"crossref","unstructured":"Somorjai RL, Dolenko B, Baumgartner R. Class prediction and discovery using gene microarray and proteomics mass spectroscopy data: curses, caveats, cautions. Bioinformatics.\u20082003;19:1484\u20131491","DOI":"10.1093\/bioinformatics\/btg182"},{"key":"2021120901584071700_R25","unstructured":"Vapnik V. Statistical Learning Theory. New York, NY: Wiley; 1998"},{"key":"2021120901584071700_R26","unstructured":"Wang XD, Wang JQ. A survey on support vector machines training and testing algorithms. Computer Engineering and Application.\u20082004;13:75\u201379"},{"key":"2021120901584071700_R27","unstructured":"Byvatov E, Schneider G. Support vector machine applications in bioinformatics. Appl Bioinformatics.\u20082003;2:67\u201377"},{"key":"2021120901584071700_R28","doi-asserted-by":"crossref","unstructured":"Jorissen RN, Gilson MK. Virtual screening of molecular databases using a support vector machine. Chem Inf Model.\u20082005;45:549\u2013561","DOI":"10.1021\/ci049641u"}],"container-title":["Pediatrics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/publications.aap.org\/pediatrics\/article-pdf\/120\/1\/e56\/1119817\/zpe00707000e56.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/publications.aap.org\/pediatrics\/article-pdf\/120\/1\/e56\/1119817\/zpe00707000e56.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T08:36:44Z","timestamp":1639039004000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.aap.org\/pediatrics\/article\/120\/1\/e56\/70543\/Detection-and-Significance-of-Serum-Protein-Marker"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,1]]},"references-count":28,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2007,7,1]]},"published-print":{"date-parts":[[2007,7,1]]}},"URL":"https:\/\/doi.org\/10.1542\/peds.2006-1364","relation":{},"ISSN":["0031-4005","1098-4275"],"issn-type":[{"type":"print","value":"0031-4005"},{"type":"electronic","value":"1098-4275"}],"subject":[],"published":{"date-parts":[[2007,7,1]]}}}