{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:11:18Z","timestamp":1791216678613,"version":"4.1.0"},"reference-count":31,"publisher":"American Academy of Pediatrics (AAP)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,11,1]]},"abstract":"\n BACKGROUND:<\/jats:title>\n We have isolated mesenchymal stem cells (MSCs) from tracheal aspirates of premature infants with respiratory distress. Under the influence of transforming growth factor \u03b2, MSCs differentiate into \u03b1-smooth-muscle actin\u2013expressing myofibroblasts. Myofibroblasts are increased in the lungs of patients with bronchopulmonary dysplasia (BPD), a chronic lung disease of prematurely born infants.<\/jats:p>\n <\/jats:sec>\n \n OBJECTIVE:<\/jats:title>\n We tested whether isolation of MSCs from tracheal aspirates of premature infants with respiratory distress during the first week of life correlates with BPD.<\/jats:p>\n <\/jats:sec>\n \n METHODS:<\/jats:title>\n Eighty-four infants born at a gestational age of &lt;33 weeks and requiring mechanical ventilation were studied. Aspirates were collected during suctioning and centrifuged. Cell pellets were resuspended in culture medium and plated. Adherent cells were grown to confluence.<\/jats:p>\n <\/jats:sec>\n \n RESULTS:<\/jats:title>\n MSCs were isolated from the tracheal aspirates of 56 infants; 28 aspirate samples showed no MSCs. There was no statistical difference in gestational age or birth weight between the MSC and no-MSC groups. In the MSC group, 12 infants died and 25 developed BPD, as defined by a requirement for supplemental oxygen at 36 weeks' postmenstrual age. In the no-MSC group, 6 infants died and 1 developed BPD. Accounting for potential influences of gender, birth weight, gestational age, number of tracheal aspirate samples taken, and the duration of endotracheal intubation (up to 7 days), isolation of MSCs increased the adjusted odds ratio of BPD more than 21-fold (95% confidence interval: 1.82\u2013265.85).<\/jats:p>\n <\/jats:sec>\n \n CONCLUSIONS:<\/jats:title>\n Isolation of tracheal aspirate MSCs predicts the development of BPD, which suggests that MSCs play an important role in the pathogenesis of this disease.<\/jats:p>\n <\/jats:sec>","DOI":"10.1542\/peds.2009-3445","type":"journal-article","created":{"date-parts":[[2010,10,12]],"date-time":"2010-10-12T01:11:41Z","timestamp":1286845901000},"page":"e1127-e1133","source":"Crossref","is-referenced-by-count":91,"title":["Isolation of Tracheal Aspirate Mesenchymal Stromal Cells Predicts Bronchopulmonary Dysplasia"],"prefix":"10.1542","volume":"126","author":[{"given":"Antonia P.","family":"Popova","sequence":"first","affiliation":[{"name":"Departments of Pediatrics and Communicable Diseases,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Bozyk","sequence":"additional","affiliation":[{"name":"Internal Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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